Effect of SBD.4A – a defined multicomponent preparation of Angelica sinensis – in periodontal regeneration models

Abstract

Periodontitis is a major cause of tooth motility and loss, resulting in destruction of the supporting structures of the tooth, including periodontal ligaments and alveolar bone. Periodontal surgery can slow the progression of the disease, but is costly, invasive, limited by contraindications and technique-sensitive. Recently, non-invasive pharmacological treatments using proteinaceous biologicals have become available. Here, for the first time, the bone-regenerative capabilities of a non-proteinaceous biological--SBD.4A--a novel, stable multicomponent growth factor isolated from a medicinal plant Angelica sinensis are reported. SBD.4A was tested in osteoblast proliferation and differentiation systems, as well as in a fibroblast-secreted hyaluronic acid assay. Furthermore, SBD.4A was formulated in a slow release matrix and tested in the rat calvarial defect model. Apart from the previously reported strong stimulation of angiogenesis, fibroblast growth and collagen synthesis--the activities needed for periodontal regeneration--SBD.4A enhanced the deposition of hyaluronic acid and proliferation of osteoblasts in vitro, as well as bone regeneration in the rat calvarial defect model. Together, these results indicate the beneficial effect of SBD.4 on periodontal ligament and bone regeneration making the case for further development of this botanical growth factor.

Immuno-histologic and histomorphometric evaluation of Angelica sinensis adjunctive to ß-tricalcium phosphate in critical-sized class II furcation defects in dogs

“…The thick fiber bundles of PDL in experimental group of 8 weeks reflects the stimulating effect of Angelica sinensis on fibroblast cells. These results are in accordance with the results of studies by Zhao H et al [ 34 , 35 ] in which SBD.4A-a defined multicomponent preparation of Angelica sinensis has stimulated inflammatory healing responses mediated by human periodontal ligament cells, through the proliferation of fibroblast cells which in turn increased the amount of produced hyaluronic acid.…”

The Use of Angelica Sinensis and β-tricalcium Phosphate in the Management of Surgically Induced Critical-sized Class II Furcation Defects in Dogs

“…The thick ber bundles of PDL in experimental group of 8 weeks re ects the stimulating effect of Angelica sinensis on broblast cells. These results are in accordance with the results of studies by Zhao H et al [35,36]in which SBD.4A-a de ned multicomponent preparation of Angelica sinensis has stimulated in ammatory healing responses mediated by human periodontal ligament cells, through the proliferation of broblast cells which in turn increased the amount of produced hyaluronic acid.…”

Effects of Plants on Osteogenic Differentiation and Mineralization of Periodontal Ligament Cells: A Systematic Review

“…These components may exhibit antioxidant and antiinflammatory effects (Chib et al ., ; Baharfar et al ., ). Several plants have also demonstrated antimicrobial, wound healing, and differentiation potential, as well as enhanced gene expression for osteogenic activities (Inaba et al ., ; Zhao et al ., ; Jeong et al ., ; Zhang et al ., ).…”