Effect of Seed Tuber Weights on the Development of Tubers and Flowering Spikes in Japanese Yams (Dioscorea japonica) Grown under Different Photoperiods and with Plant Growth Regulators

康徳, 吉田; 春實, 高橋; 啓臣, 神田; 耕基, 金浜

doi:10.2503/jjshs.76.230

Cited by 5 publications

(2 citation statements)

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“…The expansion stage can be divided into three periods: early expansion stage, middle expansion stage, late expansion stage [2,3]. Tuber morphogenesis is a complex physiological process regulated by heredity, environment, and hormones [4]. Great efforts have been made to explore the physiological factors affecting the morphogenesis of yam tubers.…”

Section: Introductionmentioning

confidence: 99%

Integrated mRNA and miRNA transcriptome analysis reveals a regulatory network for tuber expansion in Chinese yam (Dioscorea opposita)

et al. 2020

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Background: Yam tuber is a storage organ, derived from the modified stem. Tuber expansion is a complex process, and depends on the expressions of genes that can be influenced by environmental and endogenous factors. However, little is known about the regulatory mechanism of tuber expansion. In order to identify the genes and miRNAs involved in tuber expansion, we examined the mRNAs and small RNAs in Dioscorea opposita (Chinese yam) cv. Guihuai 16 tuber during its initiation and expansion stages. Results: A total of 14,238 differentially expressed genes in yam tuber at its expansion stage were identified by using RNA sequencing technology. Among them, 5723 genes were up-regulated, and 8515 genes were down-regulated. Functional analysis revealed the coordination of tuber plant involved in processes of cell events, metabolism, biosynthesis, and signal transduction pathways at transcriptional level, suggesting that these differentially expressed genes are somehow involved in response to tuber expansion, including CDPK, CaM, CDL, SAUR, DELLA, SuSy, and expansin. In addition, 541 transcription factor genes showed differential expression during the expansion stage at transcriptional level. MADS, bHLH, and GRAS were involved in cell differentiation, division, and expansion, which may relate to tuber expansion. Noteworthy, data analysis revealed that 22 known tuber miRNAs belong to 10 miRNA families, and 50 novel miRNAs were identified. The integrated analysis of miRNA-mRNA showed that 4 known miRNAs and 11 genes formed 14 miRNA-target mRNA pairs were co-expressed in expansion stage. miRNA160, miRNA396, miRNA535 and miRNA5021 may be involved in complex network to regulate cell division and differentiation in yam during its expansion stage. Conclusion: The mRNA and miRNA datasets presented here identified a subset of candidate genes and miRNAs that are putatively associated with tuber expansion in yam, a hypothetical model of genetic regulatory network associated with tuber expansion in yam was put forward, which may provide a foundation for molecular regulatory mechanism researching on tuber expansion in Dioscorea species.

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Section: Introductionmentioning

confidence: 99%

Integrated mRNA and miRNA transcriptome analysis reveals a regulatory network for tuber expansion in Chinese yam (Dioscorea opposita)

et al. 2020

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show abstract

“…The expansion stage can be divided into three periods: early expansion stage, middle expansion stage, late expansion stage [2,3]. Tuber morphogenesis is a complex physiological process regulated by heredity, environment, hormones, etc [4]. Great efforts have done to explore the physiological factors affecting the morphogenesis of yam tubers.…”

Section: Introductionmentioning

confidence: 99%

Integrated mRNA and miRNA transcriptome analysis reveals regulatory network of yam tuber expansion

Zhou¹,

Zhan²,

Hameed³

et al. 2019

Preprint

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Yam tuber is a storage organ, derived from modified stem. Tuber expansion is a complex process, it depends on the expression of genes that can be influenced by environmental and endogenous factors. However, little is known about the mechanism that regulates tuber expansion. In order to identify genes and miRNAs involved in tuber enlargement, we examined the transcriptome and small RNA in yam tuber during its initiation and expansion stages. A total of 14238 differentially expressed transcripts in yam tuber at its expansion stage were identified by using transcriptome technology. Among them, 5723 genes were up-regulated and 8515 genes were down-regulated, respectively. Functional analysis revealed the coordination of tuber plant involved in processes of cell events, metabolism, biosynthesis, and signal transduction pathways at transcriptional level, suggesting that these DEGs are somehow involved in response of tuber expansion. In addition, 536 transcription factor genes showed differential expression during the expansion stage at transcriptional level. REVEILLE 6-like was identified to be up-regulated in the circadian rhythm pathway. REVEILLE 6-like LHY protein, zinc finger CCCH domain-containing protein 14, and DELLA genes were up-regulated in expansion stage. Moreover, these genes were involved in circadian rhythm pathway, starch and sucrose metabolism pathway, and GA pathway by KEGG analysis respectively. Noteworthy, data analysis revealed that 23 known tuber miRNAs belong to 11 miRNA families, and 50 novel miRNAs were identified. miRNA160, miRNA396, and miRNA535 may be involved in complex network to regulate cell division and differentiation in yam during its expansion stage. The integrated analysis of miRNA-mRNA identified to be preferentially expressed in hormone signaling in expansion stage, miRNA160 was down-regulated in expansion stage, ARF18 and ARF17 were up-regulated in auxin signal transduction of tuber expansion in yam, highlighting the involvement of miRNA-mRNA in the regulation the hormone. The transcriptome and miRNA datasets presented here identified a subset of candidate genes and miRNAs that are putatively associated with tuber expansion in yam, thus a hypothetical model of genetic regulatory network associated with tuber expansion in yam was put forward, which may provide a foundation for molecular regulatory mechanism researching on tuber expansion in Dioscorea species.

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Phytohormone Profiling During Tuber Development of Chinese Yam by Ultra-high performance Liquid Chromatography–Triple Quadrupole Tandem Mass Spectrometry

Gong

Luo

Wang

et al. 2016

J Plant Growth Regul

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Effect of Seed Tuber Weights on the Development of Tubers and Flowering Spikes in Japanese Yams (Dioscorea japonica) Grown under Different Photoperiods and with Plant Growth Regulators

Cited by 5 publications

References 5 publications

Integrated mRNA and miRNA transcriptome analysis reveals a regulatory network for tuber expansion in Chinese yam (Dioscorea opposita)

Integrated mRNA and miRNA transcriptome analysis reveals a regulatory network for tuber expansion in Chinese yam (Dioscorea opposita)

Integrated mRNA and miRNA transcriptome analysis reveals regulatory network of yam tuber expansion

Phytohormone Profiling During Tuber Development of Chinese Yam by Ultra-high performance Liquid Chromatography–Triple Quadrupole Tandem Mass Spectrometry

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