Effect of selenium-enriched exopolysaccharide produced by Lactococcus lactis subsp. lactis on signaling molecules in mouse spleen lymphocytes

Abstract

Selenium-enriched exopolysaccharides (Se-EPS) produced by Lactococcus lactis subsp. lactis can be used as a safe and effective selenium (Se) supplement. Analysis of the Se content and monosaccharide components of Se-EPS demonstrated that it consisted of mannose, fucose, ribose, glucose, galactose, and arabinose with a molar ratio of 5.48 : 0.39 : 9.77 : 4.03 : 1.00 : 1.92, and an Se content of 183.263 μg g(-1). The differing effects of Se-EPS and EPS on calcium channels and certain key secondary messengers in spleen lymphocytes were examined and compared. Results showed that low-dose Se-EPS, but not EPS, increased the intracellular calcium ([Ca(2+)]i) levels of mouse spleen lymphocytes. Se-EPS also increased the expression and phosphorylation of Ca(2+)-calmodulin-dependent kinase (CaMK) II in lymphocytes. In addition, increased intracellular Ca(2+) levels were also found in the cells with blocked Ca(2+) channels. We speculated that Se-EPS enhanced the activation and proliferation of lymphocytes through calcium signaling by drawing from extracellular and intracellular stores. Low-dose Se-EPS also enhanced NO production, cAMP levels and PKA activity. We speculated that low-dose Se-EPS may activate certain pathways, including the calcium channel, NO, cAMP, and PKA related pathways.

Immunomodulating effects of casein-derived peptides QEPVL and QEPV on lymphocytes in vitro and in vivo

“…Crude extracts were mixed with isopyknic RPMI 1640 medium, aer which lymphocytes were pelleted by centrifugation at 1500 g for 10 min at 4 C. RPMI 1640 medium was then added to obtain the lymphocyte suspension. 20…”

Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11

“…Many EPS-producing LAB species have been isolated and identified, including Streptococcus thermophilus (Lemoine et al, 1997), Lactococcus lactis (Liu et al, 2013), L. casei, L. rhamnosus, L. plantarum, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus brevis, Lactobacillus curvatus, Lactobacillus helveticus, Lactobacillus johnsonii (Oleksy and Klewicka, 2018), and Lactobacillus fermentum (Ale et al, 2016).…”

Exopolysaccharide fraction from Pediococcus pentosaceus KFT18 induces immunostimulatory activity in macrophages and immunosuppressed mice

“…Lymphocytes play an important role in the immune responses and the activation of lymphocytes is related with the proliferation and release of cytokines (Liu et al . ). Previous research indicated that some bacterial EPS can influence the lymphocyte population and cytokine production pattern (Liu et al .…”

“…Previous research indicated that some bacterial EPS can influence the lymphocyte population and cytokine production pattern (Liu et al . ; Hidalgo‐Cantabrana et al . ; Matsuzaki et al .…”