Effect of Selenium Supplementation on Apoptosis and Cell Cycle Blockage of Renal Cells in Broilers Fed a Diet Containing Aflatoxin B1

Yu, Zhengqiang; Wang, Fengyuan; Liang, Na; Wang, Chuhan; Peng, Xi; Fang, Jing; Cui, Hengmin; Mughal, Muhammad Jameel; Lai, Weimin

doi:10.1007/s12011-015-0344-1

Cited by 40 publications

(33 citation statements)

References 56 publications

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“…The apoptotic cells could be evaluated by Flow cytometry and TUNEL assay [ 38 , 39 ]. Our present research revealed an increased apoptosis in the AFB 1 group demonstrated by TUNEL and flow cytometry, suggesting that AFB 1 could lead excess apoptosis in the chickens’ splenocytes, in line with previous researches in thymocytes [ 19 , 22 ], bursa of Fabricius cells [ 27 ] and renal cells [ 26 ]. Lymphocytes are the main components within the lymphoid organs, and severe lymphocyte depletion in the lymphoid organs was due to apoptosis [ 40 ].…”

Section: Discussionsupporting

confidence: 92%

“…Apoptosis has an important role in deveolpment, differentiation, proliferation and homeostasis of cell, tissue and organ [ 35 ]. AFB 1 directly or indirectly activated apoptotic process [ 36 , 37 ], inducing apoptosis of several poultry and mammal cells [ 8 , 9 , 17 , 19 , 21 – 26 ]. The apoptotic cells could be evaluated by Flow cytometry and TUNEL assay [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Apoptosis is associated with the normal development and homeostasis of animal tissues, and also involves in the pathogenesis [ 20 ]. Available information revealed that AFB 1 caused excessive apoptosis of several poultry and mammal cells such as hepatocytes [ 21 ], thymocytes [ 19 , 22 ], splenocytes [ 17 ], bursa of Fabricius cells [ 8 ], bronchial epithelial cells [ 23 ], jejunal mucosal cells [ 24 ], bone marrow cells [ 25 ], and renal cells [ 26 ]. Furthermore, our early researches have shown the possible link between mitochondrial molecules and apoptosis of hepatocytes [ 21 ], thymocytes [ 19 ], and bursa of Fabricius cells provoked by AFB 1 [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

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Aflatoxin B1 affects apoptosis and expression of death receptor and endoplasmic reticulum molecules in chicken spleen

et al. 2017

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Aflatoxin B1 (AFB1) is a natural product of the Aspergillus genus of molds, which grow on several foodstuffs stored in hot moist conditions, and is among the most potent hepatocarcinogens and immunosuppression presently known. The latter was related to the up-regulated apoptosis of immune organs. However, the effect of expression of death receptor and endoplasmic reticulum molecules in AFB1-induced apoptosis of chicken splenocytes was largely unknown. The objective of this study was to investigate this unknown field. One hundred and forty four one-day-old chickens were randomly divided into control group (0 mg/kg AFB1) and AFB1 group (0.6 mg/kg AFB1), respectively and fed with AFB1 for 21 days. Histological observation demonstrated that AFB1 caused slight congestion and lymphocytic depletion in the spleen. TUNEL and flow cytometry assays showed the excessive apoptosis of splenocytes provoked by AFB1. Moreover, quantitative real-time PCR analysis revealed that AFB1 induced the elevated mRNA expression of Fas, FasL, TNF-α, TNF-R1, Caspase-3, Caspase-8, Caspase-10, Grp78 and Grp94 in the spleen. These findings suggested that AFB1 could lead the excessive apoptosis and alter the expression of death receptor and endoplasmic reticulum molecules in chicken spleen.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aflatoxin B1 affects apoptosis and expression of death receptor and endoplasmic reticulum molecules in chicken spleen

et al. 2017

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“…Scott et al [2] have demonstrated that AFB 1 treatment can lead to an accumulation of chicken thymocytes in G 2 /M phase in vitro . Our previous studies suggest that AFB 1 induced jejunal cell arrest at G 2 /M phase and renal cell arrest at G 0 /G 1 phase in broilers [12, 13]. …”

Section: Introductionmentioning

confidence: 99%

The molecular mechanism of G2/M cell cycle arrest induced by AFB1 in the jejunum

et al. 2016

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Aflatoxin B1 (AFB1) has potent hepatotoxic, carcinogenic, genotoxic, immunotoxic and other adverse effects in human and animals. The aim of this study was to investigate the molecular mechanism of G2/M cell cycle arrest induced by AFB1 in the jejunum of broilers. Broilers, as experimental animals, were fed 0.6 mg/kg AFB1 diet for 3 weeks. Our results showed that AFB1 reduced the jejunal villus height, villus height/crypt ratio and caused G2/M cell cycle arrest. The G2/M cell cycle was accompanied by the increase of ataxia telangiectasia mutated (ATM), p53, Chk2, p21 protein and mRNA expression, and the decrease of Mdm2, cdc25C, cdc2, cyclin B and proliferating cell nuclear antigen protein and mRNA expression. In conclusion, AFB1 blocked G2/M cell cycle by ATM pathway in the jejunum of broilers.

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“…However, dietary addition of selenium yeast partially counteracted the negative effects of AFB 1 on weight gain and death rate [ 22 ]. Dietary Se could relieve liver lesion [ 23 ] and immunosuppression [ 24 ] induced by AFB 1 , and could protect renal cells from oxidative stress in vitro [ 25 ], and inhibit AFB 1 -induced apoptosis and cell cycle blockage in renal cells of broiler [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Sodium Selenite on Pathological Changes and Renal Functions in Broilers Fed a Diet Containing Aflatoxin B1

Liang

Wang

Peng

et al. 2015

IJERPH

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To evaluate the renal toxicity of dietary aflatoxin B1 (AFB1) and ameliorating effects of added dietary sodium selenite in broiler, renal histopathological changes, ultrastructural changes, and renal function parameters were monitored at 7, 14, and 21 days of age. Two hundred one-day-old healthy male Avian broilers were divided into four groups, namely control group, AFB1 group (0.3 mg/kg AFB1), +Se group (0.4 mg/kg Se), and AFB1+Se group (0.3 mg/kg AFB1+0.4 mg/kg Se). Compared with that of the control group, the relative weight of kidney was increased in the AFB1 group. There were no significant differences between the AFB1+Se group and the control group. By histopathological observation, the renal epithelia were swelling and necrosis at 7 and 21 days of age. Ultrastructurally, the lipid droplets and expanded endoplasmic reticulum appeared in the plasma of epithelia cells in the AFB1 group. Enlarged mitochondria with degenerated cristae were observed in the +Se group. Compared with the control group, the contents of serum creatinine and serum uric acid in the AFB1 group were increased, while the activity of renal Na+-K+ ATPase was decreased. When 0.4 mg/kg selenium was added into the diet containing 0.3 mg/kg AFB1, there were no obvious histological changes in the AFB1+Se group, and the contents of the serum creatinine and serum uric acid contents and the activity of renal Na+-K+ ATPase were close to those in the control group. In conclusion, sodium selenite exhibited protective effects on AFB1-induced kidney toxicity in broilers.

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Effect of Selenium Supplementation on Apoptosis and Cell Cycle Blockage of Renal Cells in Broilers Fed a Diet Containing Aflatoxin B1

Cited by 40 publications

References 56 publications

Aflatoxin B1 affects apoptosis and expression of death receptor and endoplasmic reticulum molecules in chicken spleen

Aflatoxin B1 affects apoptosis and expression of death receptor and endoplasmic reticulum molecules in chicken spleen

The molecular mechanism of G2/M cell cycle arrest induced by AFB1 in the jejunum

Effect of Sodium Selenite on Pathological Changes and Renal Functions in Broilers Fed a Diet Containing Aflatoxin B1

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