Effect of seminal plasma on the cryopreservation of equine spermatozoa

Moore, Amanda; Squires, E.L.; Graham, James K.

doi:10.1016/j.theriogenology.2004.05.032

Cited by 115 publications

(86 citation statements)

References 16 publications

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“…Especula-se que algumas proteínas componentes do plasma seminal, como as proteínas com afinidade pela heparina, têm papel fundamental no processo de capacitação espermática e também influenciam a resistência dos espermatozoides à criopreservação, podendo estar relacionadas com as diferenças individuais na criorresistência do sêmen (Manjunath et al, 2002;Moore et al, 2005;Souza et al, 2007). A variabilidade na qualidade do sêmen e na resistência à criopreservação é uma característica inerente à espécie canina (Threlfall, 2003;Kutzler, 2005).…”

Section: Resultsunclassified

Ejaculados individuais e pools de sêmen: diferenças em condições experimentais

Mascarenhas¹,

Xavier²,

Júnior³

2014

Arq. Bras. Med. Vet. Zootec.

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Ejaculados individuais e pools de sêmen: diferenças em condições experimentais [Individual ejaculates and pooled semen: differences under experimental conditions] R.M. Mascarenhas 1 , P.R. Xavier 1 , A.P. Marques Júnior 2 1 Aluna de pós-graduação  EV-UFMG  Belo Horizonte, MG 2 Escola de Veterinária  Universidade Federal de Minas Gerais  EV-UFMG  Belo Horizonte, MG RESUMOAvaliaram-se ejaculados caninos individuais e pools de sêmen submetidos a dois tratamentos de renovação do meio diluidor. Sêmen de seis cães foi coletado, na forma de ejaculados individuais e pools de sêmen, diluído na proporção de 1:1 em meio Tris-Gema, centrifugado a 500g/10min, e o pellet ressuspendido até concentração final de 50x10 6 espermatozoides/mL. O sêmen foi resfriado a 0,26ºC/min, entre 37 e 16ºC, e 0,08ºC/min, entre 16 a 8ºC, e mantido em geladeira a 5ºC por 14 dias. No Tratamento 1, o meio diluidor foi renovado a cada seis dias, e no Tratamento 2 aos 12 dias. O sêmen foi avaliado, a cada 48 horas, quanto à motilidade espermática, utilizando-se o Sperm Class Analyser® (SCA), e quanto à integridade de membranas pelo teste hiposmótico e coloração com PI/CFDA. A formação de pools de sêmen simplificou sua manipulação, principalmente com relação ao aumento do volume da amostra disponível; no entanto, resultados obtidos a partir de ejaculados individuais mostraram diferenças entre tratamentos, não identificadas nos pools de sêmen.

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Section: Resultsunclassified

Ejaculados individuais e pools de sêmen: diferenças em condições experimentais

Mascarenhas¹,

Xavier²,

Júnior³

2014

Arq. Bras. Med. Vet. Zootec.

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“…En esta investigación se observó que los tratamientos con 10% de PS (T1, T3, T5 y T7) presentaron en la mayoría de los casos mejores resultados de MT y MP, que sus tratamientos equivalentes con 20% de PS (T2, T4, T6 y T8) (p<0,05). Otros investigadores han registrado un efecto deletéreo del PS en la congelación de semen equino, cuando se suplementa en proporciones iguales o superiores al 20% (Alghamdi et al, 2002;Moore et al, 2005). Sin embargo, la calidad post-descongelación del semen también depende de la interacción entre los suplementos empleados en el diluyente, dado que con el uso de DMF y 20% de PS se hallaron resultados superiores para MT y MP (tabla I), así como para MA, VE y HOS (figura 1), cuando se adicionó un 5% de YHC (T8) en lugar de un 2% de este suplemento (T6) (p<0,05).…”

Section: Parámetrounclassified

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

2017

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Palabras clave adicionalesCalidad seminal. Crioprotectores. Plasma seminal. Yema de huevo. Criopreservación. resUMenLa congelación de semen de asno permite almacenar material genético por un periodo ilimitado de tiempo, por lo que puede ser empleado para generar bancos de germoplasma o para la cría de mulares y asnos. Esta investigación tuvo como objetivo evaluar alternativas de suplementación de un diluyente para la congelación de semen de asno. Se colectó el semen de cinco asnos criollos colombianos, dos eyaculados por animal (n= 10) y luego se congeló en diluyente Equiplus® suplementado con 3% de glicerol (GLY) ó 5% de dimetilformamida (DMF), 2% ó 5% de yema de huevo centrifugada (YHC) y 10% ó 20% de plasma seminal (PS). Se realizó la evaluación post-descongelación de la calidad seminal y de la peroxidación lipídica (PL). Para el análisis estadístico se ajustaron modelos mixtos, se realizó un análisis de regresión y comparaciones de medias por la prueba de Tukey. El semen congelado con 5% de DMF, 5% de YHC y 10% de PS presentó valores superiores de movilidad, vitalidad e integridad de membrana (p<0,05). La PL fue menor en diluyentes con 5% de YHC (p<0,05). La suplementación del diluyente de congelación con una combinación de 5% de DMF, 5% de YHC y 10% de PS permite mejorar la calidad post-descongelación del semen de asno. Effect of extender supplementation on the post-thaw semen quality of donkey sUMMarY Freezing of donkey semen allows storing genetic material for an unlimited period of time, which can be used to generate germoplasm banks or for breeding of mules or asses. This research aimed to evaluate supplementation alternatives for freezing donkey semen. Semen from five Colombian creole donkeys (n= 10) was collected and then frozen in Equiplus® extender supplemented with 3% of glycerol (GLY) or 5% of dimethylformamide (DMF), 2% or 5% of centrifuged egg yolk (CEY) and 10% or 20% of seminal plasma (SP). Post-thaw evaluation of semen quality and lipid peroxidation (LP) was conducted. For statistical analysis mixed models were adjusted, regression analysis and comparison of means by the Tukey test were performed. Frozen semen with DMF, 5% CEY and 10% SP presented superior results of motility and membrane integrity (p<0.05). The LP was lower in extenders with 5% CEY (p<0.05). Supplementation of freezing extender with the combination of 5% DMF, 5% CEY and 10% SP allows to improve the post-thaw quality of asinine semen.additional keYwords

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“…Hence, high SP concentrations decrease sperm quality, while limited exposure of ejaculated spermatozoa to SP prior to semen processing is beneficial for motility and cell survival (Amann & Pickett, 1987;Varner et al, 1987;Moore et al, 2005). However, a minimum SP concentration seems to be necessary for sperm survival and protection against premature capacitation during cooled storage.…”

Section: Introductionmentioning

confidence: 99%

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

2015

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SUMMARYPreservation of epididymal spermatozoa is important to save genetic material of endangered species and breeds, or in case of unexpected injury, which will end the breeding career of valuable sires. Seminal plasma (SP) influences sperm quality in a dosedependent manner and its addition to preserved semen immediately before insemination may be beneficial for sperm fertility. Increased plasma membrane stability of epididymal spermatozoa reduces freezing injury of cells, and the addition of SP after freezing and thawing might have activating and protecting effects on spermatozoa within the female genital tract. In this study, epididymal spermatozoa were harvested by retrograde flush of the epididymal cauda immediately after routine castration and frozen. Seminal plasma was collected from other six stallions. Homologous SP (SP from the same species, but from a different animal) was added to frozen-thawed epididymal spermatozoa at concentrations of 0, 5, 20, 50 and 80% SP. Addition of SP increased sperm motility and influenced kinematic values in a dose-dependent manner (p < 0.05). Motility improved at concentrations of 20 and 50% SP, but did not further increase at 80% SP. There was no difference in sperm motility among SP from six different donor stallions regardless of the concentrations of SP (p > 0.05). Total and progressive motility of ten frozen-thawed epididymal spermatozoa samples collected from different stallions after dilution with extender and 5, 20, 50 or 80% SP differed significantly (p < 0.05). In conclusion, addition of homologous SP to frozen-thawed stallion epididymal spermatozoa immediately improved motility in a dose-dependent manner regardless of semen quality of SP donor stallions. This might positively influence fertility when SP is added before insemination. Moreover, there seems to be a threshold level of SP concentration for optimal improvement of sperm motility.

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Effect of seminal plasma on the cryopreservation of equine spermatozoa

Cited by 115 publications

References 16 publications

Ejaculados individuais e pools de sêmen: diferenças em condições experimentais

Ejaculados individuais e pools de sêmen: diferenças em condições experimentais

Efecto de la suplementación del diluyente sobre la calidad del semen de asno a la descongelación

Dose-dependent effects of homologous seminal plasma on motility and kinematic characteristics of post-thaw stallion epididymal spermatozoa

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