Effect of sevoflurane pretreatment in relieving liver ischemia/reperfusion-induced pulmonary and hepatic injury

Abstract

Purpose To investigate the effect of sevoflurane preconditioning on ischemia/reperfusion (I/R)-induced pulmonary/hepatic injuryMethods Fifty-one Wistar rats were randomly grouped into sham, I/R, and sevoflurane groups. After reperfusion, the structural change of the lung was measured by Smith score, the wet and dry weights (W/D) were determined, malondialdehyde (MDA) myeloperoxidase (MPO) content was determined colorimetrically and by fluorescence, respectively, and matrix metalloprotein-9 (MMP-9) mRNA was quantified by RT-PCR. Biopsy and morphological analyses were performed on liver tissue, activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determined, and tumor necrosis factor-alpha (TNF-α) level was determined.Results The sham group showed no changes in tissue structure. Structural lesions in the sevoflurane and I/R groups were mild and severe, respectively. Smith score, W/D, MDA, MPO, and MMP mRNA showed the same trend, and were increased in the I/R group and recovered in the sevoflurane group, compared with the sham group (both P<0.05). AST and ALT were significantly increased compared to the sham group (AST: 655±52.06 vs . 29±9.30 U/L; ALT: 693±75.56 vs . 37±6.71 U/L; P<0.05). In the sevoflurane group, AST and ALT levels were significantly decreased (464±47.71 and 516±78.84 U/L; P<0.001). TNF-α presented similar results.Conclusion The protection of lung and liver by sevoflurane may be mediated by inhibited leukocyte recruitment and MMP-9 secretion.

Donor Preconditioning with Inhaled Sevoflurane Mitigates the Effects of Ischemia-Reperfusion Injury in a Swine Model of Lung Transplantation

“…Volatile anesthetics may decrease IRI-induced cytokine-mediated lung injury during thoracic procedures, in particular TNF- α , IL-1 β , IL-6, IL-8, and IL-10 [ 21 , 25 – 27 ]. Xu et al showed in a rat model that SEV preconditioning had a protective effect on IRI-induced pulmonary injury, by inhibiting leukocyte recruitment and MMP-9 secretion [ 17 ]. Ohsumi et al suggested that preconditioning or postconditioning of the lungs with SEV can display a protective effect against IRI injury in a rat LTx model.…”

“…In LTx, preconditioning of lungs with inhaled SEV has been associated with reduced IRI injury in ex vivo models of isolated rat lungs [ 16 ], in an in vivo model of rat pulmonary and hepatic injury [ 17 ], and in an in vivo model of swine autotransplantation [ 18 ]. Kalb et al showed that preconditioning but not postconditioning with SEV has beneficial effects in a rat IRI model [ 19 ].…”

Aucubin Attenuates Liver Ischemia-Reperfusion Injury by Inhibiting the HMGB1/TLR-4/NF-κB Signaling Pathway, Oxidative Stress, and Apoptosis

“…member of the antioxidant metalloenzymes (Xu et al, 2019). MDA, SOD, and ROS are able to directly respond to oxidative stress (Abdel-Gaber et al, 2019).…”

“…MDA is the most important metabolite of membrane lipid peroxidation, which can aggravate cell membrane damage and alter the activity of key enzymes in the mitochondria. Meanwhile, SOD is a vitally important member of the antioxidant metalloenzymes ( Xu et al., 2019 ). MDA, SOD, and ROS are able to directly respond to oxidative stress ( Abdel-Gaber et al., 2019 ).…”

“…Its clinical manifestation occur after the liver regains its blood supply with patients presenting a series of deterioration phenomena, such as liver function injury, jaundice, and even multiple organ failure (Donadon et al, 2016). The pathogenesis of IRI is closely related to aseptic inflammatory response, oxidative stress level, energy metabolism disorder, and cell apoptosis and autophagy (Go et al, 2015;Li et al, 2015;Olthof et al, 2017;Kan et al, 2018;Xu et al, 2019).…”