2020
DOI: 10.21203/rs.3.rs-51874/v1
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Effect of Silica-Coated Magnetic Nanoparticles on Rigidity Sensing of Human Embryonic Kidney Cells

Abstract: Background: Nanoparticles (NPs) can enter cells and cause cellular dysfunction. For example, reactive oxygen species generated by NPs can damage the cytoskeleton and impair cellular adhesion properties. Previously, we reported that cell spreading and protrusion structures such as lamellipodia and filopodia was reduced when cells are treated with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate (MNPs@SiO2(RITC)), even at 0.1 μg/μL. These protruded structures are involved in a cell’s… Show more

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Cited by 7 publications
(10 citation statements)
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“…The broad peak between 20° and 40° indicated amorphous silica beads [ 25 ]. Additionally, we validated whether the non-specific biological effects of MNPs@SiO 2 (RITC) treatment were induced by the silica shell rather than the CoFe 2 O 4 core in HEK293 cells because the silica is in the periphery of MNPs@SiO 2 (RITC) and directly interacts with biological substances [ 14 , 20 , 51 , 52 ]. Ag NPs, Au NPs, and CdSe QDs were generated as in previous studies [ 53 , 54 ].…”
Section: Methodsmentioning
confidence: 99%
“…The broad peak between 20° and 40° indicated amorphous silica beads [ 25 ]. Additionally, we validated whether the non-specific biological effects of MNPs@SiO 2 (RITC) treatment were induced by the silica shell rather than the CoFe 2 O 4 core in HEK293 cells because the silica is in the periphery of MNPs@SiO 2 (RITC) and directly interacts with biological substances [ 14 , 20 , 51 , 52 ]. Ag NPs, Au NPs, and CdSe QDs were generated as in previous studies [ 53 , 54 ].…”
Section: Methodsmentioning
confidence: 99%
“…We have previously analysed the effects of MNPs@ SiO 2 (RITC) on HEK293 cells [4,21,37,38]. In addition, HEK293 cells have also been extensively used in the study of nanoparticle-induced toxicity and in renal toxicity models [39,40].…”
Section: Introductionmentioning
confidence: 99%
“…2). The biological changes of HEK293 cells were induced by the shell (silica) rather than the cobalt ferrite core of MNPs@SiO 2 (RITC) [8,[18][19][20][21][22]. MNPs@SiO 2 (RITC) uptake was plateaued at 0.1 μg/μl MNPs@SiO 2 (RITC) treatment and cell viabilities of BV2 cell line and primary rat microglia werẽ 80% reduced at 1.0 μg/μl MNPs@SiO 2 (RITC) treatment (data not shown).…”
Section: Methodsmentioning
confidence: 94%