Effect of Silver Nanoparticles on Healing of Third-Degree Burns Infected with Pseudomonas Aeruginosa in Laboratory Mice

Abstract

The treatment of full-thickness skin burn using nanomaterials is promising as a medical application reducing the risk of infection and severe dermal scarring. Therefore, this study aims to evaluate the effectiveness of nanomaterials, particularly 3% silver nanoparticles containing ointment (3% SNO), on the full-thickness skin burn of laboratory mice. A total number of 36 male mice were used, equally divided into three groups: negative control (not burned and not treated); positive control (+ve) (burned and treated with castor oil and white petroleum jelly); and SNO-treated group (burned and treated with 3% SNO). The skin of the animals’ back was shaved. A 2x0.5 cm metal plate was heated on a burner to burn the skin of the animals of positive control and SNO-treated groups. Pseudomonas aeruginosa bacterial suspension was applied to the burnt area. The application of SNO, as well as the mixture of white petroleum jelly and castor oil, was started after 6 hours of inducing burns and continued for 14 days (three times daily) in the respected groups. The SNO-treated group showed accelerated healing within 14 days demonstrated by re-epithelialization of the epidermal layer and proliferation of the fibroblasts in the dermal layer. Less healing evidence was observed in the +ve control group in the same period. In conclusion, to our knowledge, this is the first study that uses a 3% SNO formula and has found that it has a promising impact on the treatment of infected skin burns.

The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

“…Besides, AgNPs are effective at killing and preventing the growth of bacteria of both positive and negative Grams, such as E. coli (diarrhea), S. aureus (skin infection) and P. aeruginosa (otitis media), which shows their promising usefulness in various applications [ 22 ]. Significantly, AgNPs have also been shown to have a potential in treating full-thickness skin burn in mice [ 23 ]. However, many controversies have emerged around the use of silver and AgNPs in medical applications, especially in burn treatment, and that misusing AgNPs may cause adverse effects.…”