Effect of silver nanoparticles on wettability, anti-fungal effect, flexural strength, and color stability of injection-molded heat-cured polymethylmethacrylate in human saliva

Abstract

Aim: Aim of this study was to evaluate the effect of silver nanoparticles incorporated injection molded heat-cured polymethylmethacrylate resin on wettability, anti-fungal effect, flexural strength and colour stability in human saliva. Settings and Design: An In-Vitro study with In -Vivo parameter Materials and Methods: Rectangular and circular stainless-steel dies were fabricated according to ISO standardization 20795-1:2018 and ADA specification number 12. A total of 144 samples were prepared and divided into 4 groups with thirty-six samples in each group. Each of the 4 groups were subdivided into 3 subgroups based on concentration of silver nanoparticles as 0% in subgroup A, 0.05% in subgroup B and 0.2% in subgroup C. Group 1 samples evaluated wettability, they were assessed at 0, 7, 90 and 180 days after immersing in human saliva using goniometer. Group 2 samples evaluated antifungal effect, they were assessed against Candida albicans in Muller hinton agar plate enriched with 2% glucose. Group 3 samples evaluated flexural strength, they were assessed by using universal testing machine. Group 4 samples evaluated colour stability, they were assessed using UV spectrophotometer at 0, 3 and 7 days after immersing in human saliva. Statistical Analysis Used: One-way ANOVA and Post- Hoc Tukey test were used to evaluate the significant differences in the mean values of the groups. Results: Subgroup C samples with 0.2% Ag nanoparticles had better wettability, maximum antifungal property, highest flexural strength and good colour stability followed by subgroup B and subgroup A samples. Conclusion: Injection molded denture base resin incorporated with 0.2% Ag nanoparticles could be used clinically as a denture base material for completely and partially edentulous patients.

Comparison of titanium dioxide nanoparticles and silver nanoparticles for flexural strength once incorporated in heat-cure acrylic denture base resin: An in vitro Study

“…They have an integrated redox reaction mechanism that enhances their photoactivity that can be efficiently activated over time. [ 29 30 31 ] A few studies suggest that the addition of these nanoparticles can improve the flexural strength[ 32 ] whereas others contradict them. [ 33 ]…”

Comparative Evaluation of the Flexural Strength of Heat-Activated Polymethyl Methacrylate Denture Base Resin With and Without 0.2% by the Weight of Silver Nanoparticles Cured by Conventional and Autoclave Methods: An In Vitro Study

“…The large surface area of AgNP makes it highly reactive; it can diffuse through different biological membranes and provide a bacteriocidal effect due to the inhibition of cell multiplication and damage to bacterial cells [ 9 ]. A study by Vaiyshnavi et al [ 10 ] proved that 0.2% by weight of AgNP incorporated heat-cured PMMA processed by injection molding technique provided maximum antifungal effect. Koroglu et al [ 11 ] evaluated the mechanical properties of denture base resins after adding different concentrations of AgNP.…”

“…Since it was proved in the earlier studies that 0.2% by weight AgNP incorporation into the denture base was effective against biofilm formation and preventing denture stomatitis [ 10 ], the alteration in flexural strength on the incorporation of 0.2% AgNP by weight and the effect of the autoclave method of terminal boiling on flexural strength were investigated in the present study. The primary objective was to find the difference in flexural strength of heat-activated PMMA (unmodified heat-activated PMMA) polymerized using the conventional method of terminal boiling and the autoclave method of terminal boiling.…”