Effect of Silver Nitrate and Amino Acids on High Frequency Plants Regeneration in Barley (Hordeum vulgare L.)

Haque, Ziaul; Siddique, Abu Baker; Islam, SM Shahinul

doi:10.3329/ptcb.v25i1.24124

Cited by 16 publications

(5 citation statements)

References 15 publications

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“…Among many compounds used in the in vitro anther culture, Cu 2+ and Ag + are being considered as promising [53,54]. There are shreds of evidence that the ions may increase the number of green regenerants in cereals [55,56]. However, the action of the ions may depend on their concentration in the medium as the cell wall components, i.e., cellulose may absorb them limiting their availability for the proper functioning of the respiration pathway [57].…”

Section: Discussionmentioning

confidence: 99%

Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants

Bednarek

Orłowska

2020

Plants

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Plant anther culture allows for the regeneration of uniform and homozygous double haploids. However, off-type regenerants may appear as a result of so-called tissue culture-induced variation (TCIV). In addition, the presence of Cu2+ and Ag+ ions in the culture medium might influence the number of green plants. The regenerants were obtained via anther cultures of barley under varying Cu2+ and Ag+ ion concentrations in the induction medium during distinct time conditions. DArTseqMet markers were evaluated based on regenerants and donor plants and delivering data on DNA demethylation (DM) and de novo methylation (DNM) and changes in methylation (Delta). The number of green regenerated plants per 100 anthers (GPs) was evaluated. The Cu2+ and Ag+ ion concentrations moderated relationships between Delta and the number of green plants conditional on time of tissue cultures. Depending on the ions, moderated moderation is valid within the different time of anther culture. When the highest concentration of copper is analyzed, plant regeneration is possible under short ‘Time’ (21 days) of anther culture wherein Delta is negative or under elongated Time when Delta is positive. Under 21 days of culture, the highest concentration of silver ions and when Delta is negative, some regenerants could be evaluated. However, under high Ag+ concentration when Time of culture is long and Delta positive, the highest number of green plants could be obtained.

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Section: Discussionmentioning

confidence: 99%

Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants

Bednarek

Orłowska

2020

Plants

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“…It has been reported that microspore developmental stage and some chemical substances in addition to the culture medium influenced the efficacy of embryogenesis in cereal crops [ 16 ]. Moreover, plant growth regulators have been used in induction media that helped for increasing the ability of embryogenesis in anther culture of wheat, rice and barley [ 17 , 18 , 19 ]. Some chemical substances have frequently been reported to prevent accumulation of phenolic compounds and their toxic effects on plant tissue culture phenomenon.…”

Section: Introductionmentioning

confidence: 99%

Effect of silver nitrate and growth regulators to enhance anther culture response in wheat (Triticum aestivum L.)

Hassan

Islam

2021

Heliyon

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Application of chemical substances as stress pre-treatment factors may positively influence androgenetic responses in cereal and other crops. AgNO 3 is an anti-ethylene compounds that played a significant role in combination with other chemicals for anther culture responses in cereal and other crop plants. For this study two local wheat cultivars viz. Kheri and Akbar were considered to evaluate the effect of AgNO 3 and to optimize the suitable doses of plant growth regulators, amino acids and sucrose that supplemented in MS medium. Data were recorded on the basis of embryoids induction, regenerated green and albino plants. The results clearly stated that anther culture responses and its major outcomes on regeneration significantly increased with suitable dosages of chemicals. The most noteworthy increases embryo like structures and regenerated green plants accomplished by utilizing the combined effect of AgNO 3 (50 mg/l) and as plant growth regulators IAA (1.0 mg/l) þ kinetin (0.5 mg/l). Best embryo like structures (79.17%) and green plants (33.33%) were recorded in Kheri. The results clearly stated that reducing albinism and increasing embryos induction and green plants 50-75 mg/l silver nitrate along with optimum doses of IAA and kinetin showed very effective results in wheat.

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“…AA has been shown to inhibit the browning of cultured tissues and improve morphogenesis in Cavendish banana (Ko et al, 2009) and Brachylaena huillensis (Ndakidemi et al, 2014). Similarly, AgNO 3 is a potent ethylene inhibitor and its presence in culture medium has been reported to inhibit browning and induce in vitro shoot production in many plant species (Haque et al, 2015;Kabir et al, 2013;Mookkan and Andy, 2014). Several hypotheses have been postulated on possible mechanisms of AgNO 3 in reducing explant browning and promoting plant regeneration.…”

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confidence: 99%

Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.)

Irshad¹,

Rizwan²,

Debnath³

et al. 2018

horts

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The regeneration frequency of okra (Abelmoschus esculentus) is greatly influenced by its genetic makeup and recalcitrant nature. Phenolic secretion, in particular, is a major problem in okra tissue culture. This study describes a reproducible, rapid, and more efficient in vitro regeneration method using cotyledonary node explants of okra. Explants were incubated on Murashige and Skoog (MS) medium containing different concentrations and combinations of various plant growth regulators (PGRs) [benzyladenine (BA), thidiazuron (TDZ), and α-naphthylacetic acid (NAA)], and regeneration enhancers [silver nitrate (AgNO3) and Pluronic F-68]. Cut ends of cotyledonary node segments rapidly turned brown and cultures failed to establish. Antibrowning additives, such as activated charcoal (AC), ascorbic acid (AA), and AgNO3 at various concentrations in PGR-free MS basal medium were tested for their ability to control phenolic secretion from explants. Among these additives, 15 mg·L−1 AA was found to be optimal for controlling phenolic secretion, resulting in healthy explants and culture establishment. The highest number of shoots (a mean of 9.3 ± 0.9 shoots per cotyledonary node explant) was obtained on MS media containing 0.5 mg·L−1 NAA + 1 mg·L−1 TDZ + 0.1% Pluronic F-68. Individual shoots were elongated on MS medium + 1 mg·L−1 BA + 0.1 mg·L−1 gibberellic acid (GA3) (shoot length 5.3 ± 0.2 cm) and rooted on ½ MS medium + 1 mg·L−1 indole-3-butyric acid (IBA) and 200 mg·L−1 AC (5.3 ± 0.2 roots per shoot). Rooted plantlets were acclimatized in plastic pots inside a plant growth chamber at 25 ± 2 °C and 70% relative humidity, with an 80% survival rate. This optimized protocol can be used for producing transgenic plants of commercial okra cultivars through genetic transformation.

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Effect of Silver Nitrate and Amino Acids on High Frequency Plants Regeneration in Barley (Hordeum vulgare L.)

Cited by 16 publications

References 15 publications

Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants

Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants

Effect of silver nitrate and growth regulators to enhance anther culture response in wheat (Triticum aestivum L.)

Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.)

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