Effect of single and combined treatments with MPF or MAPK inhibitors on parthenogenetic haploid activation of bovine oocytes

Suvá, M.; Canel, N. G.; Salamone, D.

doi:10.1016/j.repbio.2019.09.001

Cited by 12 publications

(8 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…activation (Naruse et al, 2007;Suvá et al, 2019). CHX, a nonspecific protein synthesis inhibitor, can inhibit the synthesis of cyclin B, which inactivates MPF and finally results in the relief of the meiosis arrest (Mori et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Due to the significant role of protein kinases and phosphatases in the fertilization process ( Adhikari and Liu, 2014 ; Tosti and Ménézo, 2016 ), it was available to combine Ca 2+ ionophores with a protein synthesis inhibitor for parthenogenetic oocyte activation ( Naruse et al, 2007 ; Suvá et al, 2019 ). CHX, a non-specific protein synthesis inhibitor, can inhibit the synthesis of cyclin B, which inactivates MPF and finally results in the relief of the meiosis arrest ( Mori et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure

Wang

Zhu

Liu

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Total fertilization failure (TFF) occurs in 1–3% of total intracytoplasmic sperm injection (ICSI) cycles and can reoccur in subsequent cycles. Despite the high success rate with the application of assisted oocyte activation (AOA), there is still a small number of couples who cannot obtain fertilized eggs after conventional calcium (Ca2+) ionophores-based ICSI-AOA. Six couples experiencing repeated TFF or low fertilization (<10%) after ICSI and conventional ICSI-AOA were enrolled in this study. Compared with the regular ICSI group and the conventional ICSI-AOA group, the new AOA method, a combination of cycloheximide (CHX) and ionomycin, can significantly increase the fertilization rate from less than 10 up to approximately 50% in most cases. The normal distribution of sperm-related oocyte activation factor phospholipase C zeta (PLCζ1) in the sperms of the cases indicated the absence of an aberrant Ca2+ signaling activation. The results of the whole-embryo aneuploidies analysis indicated that oocytes receiving the novel AOA treatment had the potential to develop into blastocysts with normal karyotypes. Our data demonstrated that CHX combined with ionomycin was able to effectively improve the fertilization rate in the majority of patients suffering from TFF. This novel AOA method had a potential therapeutic effect on those couples experiencing TFF, even after conventional AOA, which may surmount the severe fertilization deficiencies in patients with a repeated low fertilization or TFF.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure

Wang

Zhu

Liu

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Recent studies have reported similar cleavage and blastocyst rates after bovine oocyte parthenogenetic activation with 5 µM ionomycin and 2 mM 6-dimethylaminopurine [35,36]. The combination of 5 µM ionomycin, 7% ethanol (v/v), and 50 µM roscovitine improved pronucleus formation rates in parthenogenetic embryos, while the highest blastocyst yields were observed in the ionomycin and 6-dimethylaminopurine treatment [24]. However, these protocols are long, involve several steps, and are difficult to reproduce.…”

Section: Discussionmentioning

confidence: 97%

“…In addition, we isolated total RNA from parthenogenetic blastocysts, during preliminary experiments, and used it for successful qPCR standardization, indicating that parthenogenetic blastocysts had an active transcription machinery. Considering that artificial activation of bovine oocytes is a critical step for reproductive biotechnologies such as Intracytoplasmic Sperm Injection or Somatic Cell Nuclear Transfer [24], we propose this methodology for successful parthenogenetic activation of bovine oocytes. It could potentially be applied in other species as study material or stem cell source.…”

Section: Discussionmentioning

confidence: 99%

“…Due to its hydrophobic nature, α-tocopherol is first diluted in absolute ethanol, to obtain a final 25-400 µM α-tocopherol concentration and 0.05% (v/v) ethanol in culture media. Ethanol is a known parthenogenesis activator at concentrations above 3% [23], and when associated with other molecules is used in long protocols to produce parthenogenetic embryos [24]. Although 1% ethanol and 20% O 2 have been found to be inefficient in promoting parthenogenetic activation in bovine oocytes [23], a recent study reported that adding 0.3% ethanol to the IVM medium increased blastocyst rate, regardless of culture conditions [25].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Ethanol on Parthenogenetic Activation and α-Tocopherol Supplementation during In Vitro Maturation on Developmental Competence of Summer-Collected Bovine Oocytes

Báez

Gómez

Brun

et al. 2021

CIMB

View full text Add to dashboard Cite

The use of α-tocopherol during in vitro maturation (IVM) is an alternative to minimize the adverse effects of heat stress on oocyte competence. However, α-tocopherol is diluted in ethanol, which can induce oocyte parthenogenetic activation (PA). This study aimed to evaluate the role of ethanol concentration on PA and the effect of α-tocopherol supplementation during IVM on the developmental competence and the expression of key genes in blastocysts derived from summer-collected oocytes. All in vitro embryo production was conducted at 5% O2, 5% CO2 at 38.5 °C. Experiment 1: oocytes were cultured with or without 0.05% ethanol. As positive PA control matured oocytes were subjected to 3% or 7% ethanol for 7 min. Oocytes from all groups were placed in fertilization medium (22 h) and culture medium (9 days). Ethanol at 0.05% during IVM did not induce oocyte PA, however, 3% and 7% ethanol were effective parthenogenetic inductors. Experiment 2: oocytes were cultured in maturation medium supplemented with 0, 50, 100 and 200 μM α-tocopherol, diluted in 0.05% ethanol. After in vitro fertilization and embryo culture, we assessed blastocyst apoptotic index and the transcription of a panel of genes. The results showed that supplementation with 100 μM α-tocopherol reduced apoptotic index and increased the expression of SOD2. In conclusion, 100 μM α-tocopherol, diluted in 0.05% ethanol, can be used during IVM to embryonic quality.

show abstract

Chemical activation of mammalian oocytes and its application in camelid reproductive biotechnologies: A review

Bellido-Quispe,

Arcce,

Pinzón-Osorio

et al. 2024

Animal Reproduction Science

View full text Add to dashboard Cite

Effect of single and combined treatments with MPF or MAPK inhibitors on parthenogenetic haploid activation of bovine oocytes

Cited by 12 publications

References 56 publications

A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure

A Novel Assisted Oocyte Activation Method Improves Fertilization in Patients With Recurrent Fertilization Failure

Effect of Ethanol on Parthenogenetic Activation and α-Tocopherol Supplementation during In Vitro Maturation on Developmental Competence of Summer-Collected Bovine Oocytes

Chemical activation of mammalian oocytes and its application in camelid reproductive biotechnologies: A review

Contact Info

Product

Resources

About