Effect of single- and multi-strain probiotics on broiler breeder performance, immunity and intestinal toll-like receptors expression

Abstract

Effects of multi-strain (Lactofeed) and mono-strain (Pediguard) probiotics on broiler breeder performance and expression of toll-like receptors (TLR) were evaluated. Three hundred broiler breeder hens (Ross 308) at the age of 51 weeks were randomly allocated into 1 of 5 dietary treatments with 6 replicates in each in a completely randomized design. The dietary treatments included (1) the basal diet (control), ( 2) control + 0.1 g/kg Lactofeed, (3) control + 0.1 g/kg Pediguard, (4) control + 0.1 g/kg Lactofeed + 0.1 g/kg Pediguard and ( 5) control + 0.5 g/kg oxytetracycline antibiotic. Compared to the control group, treatments had no effect on hen-day egg production and body weight of broiler breeders (P > .05). The egg yolk cholesterol concentration of broiler breeders fed probiotic-supplemented diet was decreased (P < .05). There were no differences in the immune response to PHA-P injection, serum glutathione peroxidase activity, malondialdehyde and cholesterol concentration and blood haematology of broiler breeder among different dietary treatments (P > .05) while TLR2 and TLR4 mRNA expression up-regulated (P < .05). It can be concluded Lactofeed and Pediguard did not improve broiler breeder performance and T-cell-mediated immune response and are not advisable for breeder nutrition.

The effect of multi-strain probiotics as feed additives on performance, immunity, expression of nutrient transporter genes and gut morphometry in broiler chickens

“…Antibiotic growth promoters (AGP) have been used widely to enhance the production capacity of poultry and protect them from pathogen risk. But due to the residual effect of antibiotics on human health, the use of antibiotics in poultry feed is banned or going to be banned in many countries [1]. So, the potential alternatives to AGPs are required to maintain health and use as growth stimulant of poultry.…”

Preventive antimicrobial action and tissue architecture ameliorations of Bacillus subtilis in challenged broilers

“…Probiotics can exert their action through various methods, including blocking bacterial binding sites (competitive inhibition), regenerating intestinal mucosa, and enhancing the secretion of digestive enzymes. In our study, supplementing broilers with 1.5 and 2.0 g B. subtilis (5×10 6 CFU/g in each liter of drinking water) enhanced the live body weight, accelerated weight gains, and improved performance indices compared to 1.0* g B. subtilis (the quantity recommended by the manufacturer). The observed actions could be attributed to the enhancing influence of B. subtilis probiotics on intestinal permeability and absorption functions.…”

“…Alternatives such as cytokines, bacteriophages [2], Nigella sativa Linn [3], cinnamon (Cinnamomum zeylanicum) oil [4], inorganic nano-selenium [5], and probiotics [6] have been suggested and found to be effective in improving performance and immunity.…”

“…This study aims to evaluate the in vitro antimicrobial action of probiotic Bacillus subtilis (5×10 6 CFU/g) at different concentrations (0.5, 1.0*, 1.5, and 2.0 g/L) against E. coli O157: H7 (4.5×10 12 CFU/mL), S. Typhimurium (1.2×10 7 CFU/mL), Candida albicans (2.5×10 6 CFU/mL), and Trichophyton mentagrophytes (2.5×10 6 CFU/mL) after different exposure intervals (0.25, 0.5, 1, and 2 h) using minimal inhibitory concentration tests. The study also aims to study the in vivo preventive and prophylactic actions of probiotic B. subtilis on productive performance, histopathological picture, biochemical profile, intestinal microbial load, immune and edible organs' weights, and immunoglobulin (Ig) concentrations in 14-day-old broiler chickens challenged with E. coli O157: H7 (4.5×10 12 CFU/mL) and S. Typhimurium (1.2×10 7 CFU/mL).…”

Carcass and organ characteristics of finishing broilers fed diets containing probiotics (Saccharomyces cerevisiae)

“…Probiotics have been defined by several authors as live microorganisms that have beneficial effects on the host animal when administered (Ahmad, 2006;Ezema, 2013;Ritzi et al, 2014;Aalaei et al, 2019). Over the last decade, the use of probiotics in animal nutrition has gained so much attention and aroused growing research interest in the field of nutrigenomics.…”