Effect of sodium ascorbate on the chemiluminescent response of murine peritoneal exudate cells

Arquette, Deanna; Caren, Linda D.

doi:10.1016/0024-3205(92)90179-s

“…A suppressive e#ect was also demonstrated by Lygren & Waagbø (1998) when adding increasing concentrations of vitamin C to the incubation medium prior to chemiluminescent measurements of Atlantic salmon phagocytes. Furthermore, vitamin C-mediated in vitro inhibition of the chemiluminescent response has been demonstrated in murine phagocytes (Arquette & Caren, 1992). The ability of vitamin C to neutralise ROS produced by activated phagocytes could be beneficial for the fish in protecting the surrounding tissue against oxidative damage and thereby reduce some of the immunopathological e#ects associated with an infection.…”

Section: Discussionmentioning

confidence: 99%

Nutritional impacts on the chemiluminescent response of Atlantic salmon (Salmo salar L.) head kidney phagocytes, in vitro

Lygren

¹

,

Waagbø

²

1999

Fish & Shellfish Immunology

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“…An a-tocopherol acetate-enriched or -deficient diet failed to significantly activate Kupffer cell phagocytosis and to influence liver injury in rats (Rücker et al 1997). Vitamin C when incubated in vitro neutralized the free radicals produced during respiratory burst of murine peritoneal phagocytic cells, but showed no effect on ROS production in cells harvested from mice pretreated with high doses of the vitamin in vivo (Arquette & Caren, 1992).…”

mentioning

confidence: 91%

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Jakus

¹

,

Kriska

²

,

Vanyúr

³

2002

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The effect of regular intake of low doses of an effervescent multivitamin preparation on the free-radical-producing activity of murine peritoneal macrophages under conditions resembling a possible infection was studied in vitro. Initially, several groups of mice were fed a basal diet and given, for 2 weeks, water without or with supplementation of either a -tocopherol, ascorbic acid, riboflavin or a multivitamin preparation. The supplementation period was followed by a 2-week wash-out time interval during which control and multivitamin groups received deionized water. Macrophage stimulation tests using chemiluminescent spectroscopy were performed at the end of the supplementation and wash-out periods to determine cell counts and their capacity to produce free radicals. Multivitamin supplementation increased the number, and the reactive oxygen species-producing activity, of macrophages. This effect persisted for 2 weeks after higher doses of supplementation were stopped. Multivitamin supplementation lowered the steady-state free radical concentrations of liver and spleen as measured by electron paramagnetic resonance spectroscopy. It also increased the antioxidant reactivity of the same organs, while there was no effect on the free radical concentration and antioxidant capacity of the kidney and brain. When taken regularly, low doses of multivitamin supplementation may have a beneficial effect on the defence mechanisms of the organism.Vitamins: Reactive oxygen species: Chemiluminescence: Respiratory burst Nutrition has a great importance in the maintenance of immunity and health of both man and animals (for reviews see:

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“…An a-tocopherol acetate-enriched or -deficient diet failed to significantly activate Kupffer cell phagocytosis and to influence liver injury in rats (Rücker et al 1997). Vitamin C when incubated in vitro neutralized the free radicals produced during respiratory burst of murine peritoneal phagocytic cells, but showed no effect on ROS production in cells harvested from mice pretreated with high doses of the vitamin in vivo (Arquette & Caren, 1992).…”

Section: Vitamins: Reactive Oxygen Species: Chemiluminescence: Respirmentioning

confidence: 99%

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Jakus

¹

,

Kriska

²

,

Vanyúr

³

2002

British Journal of Nutrition

1

0

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The effect of regular intake of low doses of an effervescent multivitamin preparation on the free-radical-producing activity of murine peritoneal macrophages under conditions resembling a possible infection was studied in vitro. Initially, several groups of mice were fed a basal diet and given, for 2 weeks, water without or with supplementation of either a -tocopherol, ascorbic acid, riboflavin or a multivitamin preparation. The supplementation period was followed by a 2-week wash-out time interval during which control and multivitamin groups received deionized water. Macrophage stimulation tests using chemiluminescent spectroscopy were performed at the end of the supplementation and wash-out periods to determine cell counts and their capacity to produce free radicals. Multivitamin supplementation increased the number, and the reactive oxygen species-producing activity, of macrophages. This effect persisted for 2 weeks after higher doses of supplementation were stopped. Multivitamin supplementation lowered the steady-state free radical concentrations of liver and spleen as measured by electron paramagnetic resonance spectroscopy. It also increased the antioxidant reactivity of the same organs, while there was no effect on the free radical concentration and antioxidant capacity of the kidney and brain. When taken regularly, low doses of multivitamin supplementation may have a beneficial effect on the defence mechanisms of the organism.Vitamins: Reactive oxygen species: Chemiluminescence: Respiratory burst Nutrition has a great importance in the maintenance of immunity and health of both man and animals (for reviews see:

show abstract

Effect of sodium ascorbate on the chemiluminescent response of murine peritoneal exudate cells

Cited by 5 publications

References 12 publications

Nutritional impacts on the chemiluminescent response of Atlantic salmon (Salmo salar L.) head kidney phagocytes, in vitro

Nutritional impacts on the chemiluminescent response of Atlantic salmon (Salmo salar L.) head kidney phagocytes, in vitro

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

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