Effect of Sodium Nitrite and L-NNA on the Outcome of Experimental Ischemic Stroke

Kuzenkov, V. S.; Крушинский, А.Л.

doi:10.1007/s10517-015-2926-5

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…The molecular mechanism of the brain toxicity of SN is rarely studied. There are some attempts to show the histological changes of the brain tissues after chronic ingestion of SN 14,15 and neuronal toxicity of nitrogen oxide in the brain tissue 16,17 . There is a great incidence of brain cancer between teens that may be linked to the increased ingestion of preserved and processed meats.…”

Section: Discussionmentioning

confidence: 99%

Thymoquinone therapy remediates elevated brain tissue inflammatory mediators induced by chronic administration of food preservatives

Hamdan

Al‐Gayyar

Shams

et al. 2019

Sci Rep

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Continuous exposure to preservatives such as nitrite salts has deleterious effects on different organs. Meanwhile, Nigella sativa oil can remediate such organ dysfunction. Here, we studied the effect of consumption of thymoquinone (TQ); the main component of Nigella sativa oil on the brain damage induced by sodium nitrite. Forty adult male rats were daily given oral gavage of sodium nitrite (80 mg/kg) with or without thymoquinone (50 mg/kg). Oxidative stress, cytokines of inflammation, fibrotic elements and apoptotic markers in brain tissue were measured. Exposure to sodium nitrite (SN) resulted in increased levels of malondialdehyde, TGF-β, c-reactive protein, NF-κB, TNF-α, IL-1β and caspase-3 associated with reduced levels of glutathione, cytochrome c oxidase, Nrf2 and IL-10. However, exposure of rats’ brain tissues to thymoquinone resulted ameliorated all these effects. In conclusion, thymoquinone remediates sodium nitrite-induced brain impairment through several mechanisms including attenuation of oxidative stress, retrieving the reduced concentration of glutathione, blocks elevated levels of pro-inflammatory cytokines, restores cytochrome c oxidase activity, and reducing the apoptosis markers in the brain tissues of rats.

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Section: Discussionmentioning

confidence: 99%

Thymoquinone therapy remediates elevated brain tissue inflammatory mediators induced by chronic administration of food preservatives

Hamdan

Al‐Gayyar

Shams

et al. 2019

Sci Rep

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“…Regulation of vascular endothelial tension depends on NO in endothelial relaxing factors (EDRFs) which can promote vasodilation [34,37], while PGI2 has a strong vasodilator effect. L-NNA is commonly used as a model drug for hypertension [38][39][40][41][42][43][44][45][46] because the occurrence of hypertension is generally accompanied by vascular endothelial dysfunction [47], which is mainly manifested by the reduction of the endothelial vasodilator factor (EDF) production and release. e secretion of NO in VEC occurs through autocrine, endocrine, and paracrine pathways.…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology Prediction and Pharmacological Verification Mechanism of Yeju Jiangya Decoction on Hypertension

Wang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Yeju Jiangya decoction (CIF) is an herbal formula from traditional Chinese medicine (TCM) for the treatment of hypertension. Materials and Methods. Based on the analysis of network pharmacology, combined with in animal experiments, the network pharmacology was used to explore the potential proteins and mechanisms of CIF against hypertension. The bioactive compounds of CIF were screened by using the platform, and the targets of hypertension and CIF were collected. Then, the Kyoto Encyclopedia of Genes and Genomes (KEGG) and protein-protein interaction network (PPI) core targets were carried out, and the useful proteins were found by molecular docking technology. Finally, we used N-nitro-L-arginine (L-NNA) induced hypertension model rats to confirm the effect and mechanism of CIF on hypertension. Results. 14 bioactive compounds of CIF passed the virtual screening criteria, and 178 overlapping targets were identified as core targets of CIF against hypertension. The CIF-related target network with 178 nodes and 344 edges is constructed. The topological results show that quercetin and luteolin are the key components in the network. The key targets NOS3 (nitric oxide synthase 3) and NOS2 (nitric oxide synthase 2) were screened by the protein-protein interaction network. The analysis of target protein pathway enrichment showed that the accumulation pathway is related to the vascular structure of CIF regulation of hypertension. Further verification based on molecular docking results showed that NOS3 had the good binding ability with quercetin and luteolin. On the other hand, NOS3 has an important relationship with the composition of blood vessels. Furthermore, the animal experiment indicated that after the L-NNA-induced hypertension rat model was established, CIF intervention was given by gavage for 3 weeks, and it can decrease serum concentrations of endothelin-1 (ET-1) and thromboxane B2 (TXB2), increase the expression of nitric oxide (NO) and prostacyclin 2 (PGI2), and improve renal, cardiac, and aortic lesions. At the same time, it can reduce blood pressure and shorten vertigo time. Western blot (WB) and immunohistochemistry (IHC) analyses indicated that CIF may downregulate the expression of NOS3, guanylyl cyclase-alpha 1 (GC-α1), guanylyl cyclase-alpha 2 (GC-α2), and protein kinase CGMP-dependent 1 (PRKG1). These results suggest that CIF may play an antihypertensive role by inhibiting the activation of the NOS3/PRKG1 pathway. Conclusions. The results of this study indicate that CIF has the ability to improve target organs, protect endothelial function, and reduce blood pressure and that CIF might be a potential therapeutic drug for the prevention of hypertension. It provides new insight into hypertension and the potential biological basis and mechanism for CIF clinical research.

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Theoretical, Methodological and Practical Aspects of Ischemic Stroke Research as a Socio-economic Problem of Modern Healthcare

Макарова

Pogorely

Makarova

et al. 2021

Lecture Notes in Networks and Systems

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Effect of Sodium Nitrite and L-NNA on the Outcome of Experimental Ischemic Stroke

Cited by 3 publications

References 11 publications

Thymoquinone therapy remediates elevated brain tissue inflammatory mediators induced by chronic administration of food preservatives

Thymoquinone therapy remediates elevated brain tissue inflammatory mediators induced by chronic administration of food preservatives

Network Pharmacology Prediction and Pharmacological Verification Mechanism of Yeju Jiangya Decoction on Hypertension

Theoretical, Methodological and Practical Aspects of Ischemic Stroke Research as a Socio-economic Problem of Modern Healthcare

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