Effect of Solubility of Thiamine Dilauryl Sulfate Solution through the Manufacture of the Nano Paticles on Antifungal Activity

Abstract

: Conventional Thiamine Dilauryl Sulfate (TDS) powder has a low stability. In order to solve this problem, this study was performed to improve the solubility of TDS. The process for enhance solubility of TDS was nano grinding mill and ultrasonic dispersion process. TDS paticle was manufactured to nano size through nano grinding mill process. The size of TDS nanoparticle was measured as average 220 ㎚ by DLS. And The TDS nanoparticle in water solution manufactured through ultrasonic dispersion process. The TDS nanoparticle in water solution was showed the highest solubility with 40% ethanol. These results was increased the concentration of TDS from 200 ppm to 240 ppm in water solution. The TDS nanoparticle in water solution showed diameter of Colletotrichum gloeosporioides growth with smaller than about 1.56 ㎝ compared to the TDS paticle in water solution at same concentration. Also, TDS nanoparticle in water solution showed growth inhibition activity as 59.2% with higher than about 10% compared to the TDS paticle water solution in same concentration. Finally, TDS nanoparticle in water solution was increased solubility through nano grinding mill and ultrasonic dispersion process. Also, the increase of concentration in TDS nanopaticle in water solution according to solubility enhancement lead to an result enhancement of antifungal activity. Consequently, we suggested that the TDS nanoparticle in water solution was more effective than TDS particle in water solution owing to the sub-cellular particle size, ability to persistence and targeting to cell membrane of Colletotrichum gloeosporioides. Furthermore we expected the applicating possibility with bio pesticide.

Effect of Nanoencapsulated Vitamin B1 Derivative on Inhibition of Both Mycelial Growth and Spore Germination of Fusarium oxysporum f. sp. raphani

“…This result implied that the lecithin did not show a positive or negative effect on the mycelia growth for this experiment. These results suggest that using TDS nanoparticle solution can dramatically increase the surface areas acting on the mycelia of F. oxysporum , as well as the persistence of TDS in the mycelia, as opposed to using general TDS solution [13,14]. Moreover, whereas the water-dispersible powder of metalaxyl–copper oxychloride, a known chemical sterilizer, showed a 50% inhibition rate, the 100 ppm TDS nanoparticle solution showed a higher mycelial growth inhibition rate of 60% [15].…”