Effect of solvent and initial water content on (R, S)-1-phenylethanol resolution

Chua, Lee Suan; Sarmidi, Mohamad Roji

doi:10.1016/j.enzmictec.2005.07.027

Cited by 60 publications

(27 citation statements)

References 27 publications

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“…Those polar solvents with log P < 2 are often ineffective. Although log P value does not always correlate with the degree of enzymatic synthesis, it provides valuable information for solvent selection [20]. Three different types of solvent had been chosen to investigate the effect of reaction media.…”

Section: Effect Of Different Solventsmentioning

confidence: 99%

Studies of reaction parameters on synthesis of Citronellyl laurate ester via immobilized Candida rugosa lipase in organic media

Serri

Kamaruddin

Long

2006

Bioprocess Biosyst Eng

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Immobilized Candida rugosa lipase was used for the synthesis of citronellyl laurate from citronellol and lauric acid. Screening of different types of support (Amberlite MB-1 and Celite) for immobilization of lipase and solvent (n-hexane, n-heptane, and iso-octane) and optimization of reaction conditions, such as catalyst loading, effect of substrates molar ratio and temperature, have been studied. The maximum enzyme activity was obtained at 310 K. The immobilized C. rugosa lipase onto Amberlite MB-1 support was found to be the best support with a conversion of 89% of citronellyl laurate ester in iso-octane compared to Celite 545. Deactivation of C. rugosa lipase at 313, 318 and 323 K were observed. Ordered bi bi mechanism with dead end complex of lauric acid was found to fit the initial rate data and the kinetic parameters were obtained by non-linear regression analysis.

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Section: Effect Of Different Solventsmentioning

confidence: 99%

Studies of reaction parameters on synthesis of Citronellyl laurate ester via immobilized Candida rugosa lipase in organic media

Serri

Kamaruddin

Long

2006

Bioprocess Biosyst Eng

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“…Commonly, the high hydrophobic solvents are considered the most suitable solvent for biocatalysis [18]. In addition to solvent hydrophobicity, the molecular structure of the solvent and the solubility of substrate in solvent are also important [4,19].…”

Section: Effect Of Solventmentioning

confidence: 99%

Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

Xia

Wang

Yang

et al. 2009

Appl Biochem Biotechnol

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Eleven lipases are tested and it was found that lipases can be divided into three types according to water activity dependence. The first type is lipase that has low water activity dependence and works in a low water activity, its performance changes little with the change of water activity. The optimum water activity is 0.19 and Newlase F (Rhizopus niveus), lipase FAP-15 (Rhizopus oryzae) belong to this type. The second type is lipase that has medium water activity dependence and its performance changes with the change of water activity. Most lipases belong to this type and the optimum water activity in this type is about 0.60. The third type is lipase that has a high water activity dependence and works only in a high water activity (a ( w ) > 0.75). WGL (wheat germ) belongs to this type and the optimum water activity is 0.90. The relationship between enantioselectivity and water activity is also discussed and the enantioselectivity seems to be independent of water activity. And we also compared the two control methods of water activity, it was found that the method which add solid salt hydrates to the reaction mixture (method II) is more stable and effective throughout the reaction than the method that pre-equilibrate via the vapor phase (method I). The addition concentration of salt hydrates is also investigated and the optimum concentration is 1 g/l.

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“…9 On the other hand, water is essential for enzyme catalysis in order to maintain enzyme activity in organic media. 10 The hydration level of the enzyme is affected by the amount of the bound water. 8 The optimal amount of water required by an enzyme depends on the type of solvent, substrate, solid support and reaction conditions.…”

Section: Introductionmentioning

confidence: 99%

Kinetic modeling of lipase‐mediated one‐pot chemo‐bio cascade synthesis of R‐1‐phenyl ethyl acetate starting from acetophenone

Sahin

Wärnå

Mäki‐Arvela

et al. 2009

J of Chemical Tech & Biotech

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Effect of solvent and initial water content on (R, S)-1-phenylethanol resolution

Cited by 60 publications

References 27 publications

Studies of reaction parameters on synthesis of Citronellyl laurate ester via immobilized Candida rugosa lipase in organic media

Studies of reaction parameters on synthesis of Citronellyl laurate ester via immobilized Candida rugosa lipase in organic media

Water Activity Dependence of Lipases in Non-aqueous Biocatalysis

Kinetic modeling of lipase‐mediated one‐pot chemo‐bio cascade synthesis of R‐1‐phenyl ethyl acetate starting from acetophenone

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