Effect of sorting boar spermatozoa by sex chromosomes on oviduct cell binding

Effect of delaying the time of insemination with sex‐sorted semen on pregnancy rate in Holstein heifers

“…The plausible explanation for this approach, which was also observed in the previous studies (Bombardelli et al, 2016;Vazquez et al, 2003), was sorting process damage to sex-sorted sperm (Bombardelli et al, 2016;Vishwanath & Moreno, 2018;Winters et al, 2018 TA B L E 3 Effect of sex-sorted semen from different sires on pregnancy rates in Holstein heifers life span of the sperm than non-sorted sperm, acrosomal membrane alterations and early capacitation/pre-capacitation like changes (Schenk, Cran, Everett, & Seidel, 2009). The sorting procedure changed the physiology of sex-sorted sperm, and sex-sorted sperm need less time for capacitation and to be ready for fertilization in the reproductive tract compared to non-sorted sperm (Vishwanath & Moreno, 2018;Winters et al, 2018). Therefore, delaying insemination with sex-sorted semen at closer to ovulation time was a critical approach to get better fertility (Hall, Kasimanickam, Glaze, & Roberts-Lew, 2017).…”

“…Delaying the time of insemination with sex‐sorted semen resulted in significantly higher pregnancy rate in Holstein heifers in this study. The plausible explanation for this approach, which was also observed in the previous studies (Bombardelli et al., 2016; Vazquez et al., 2003), was sorting process damage to sex‐sorted sperm (Bombardelli et al., 2016; Vishwanath & Moreno, 2018; Winters et al., 2018). Exposure to damage during the sorting procedure resulted in shorter life span of the sperm than non‐sorted sperm, acrosomal membrane alterations and early capacitation/pre‐capacitation like changes (Schenk, Cran, Everett, & Seidel, 2009).…”

“…Temperature-humidity index (THI) was also calculated to determine the effect of heat stress using daily Meteorological Service forecasts. When the maximum THI value was above 65 for threshold at the time of insemination, heat stress was considered to be effective (De Rensis, Garcia-Ispierto, & López-Gatius, 2015;Gernand, König, & Kipp, 2019;West, 2003).…”

Bovine sperm sex‐selection technology in Japan

“…It has been reported that capacitated sperm are less able to bind to the oviduct epithelial cells in bulls and pigs . Indeed, sex‐sorted porcine sperm showed less ability to bind to the oviduct epithelial cells . The results of studies that have been performed thus far indicate that the sperm life span in sexed semen is shortened by sorting damage, which causes capacitation‐like membrane changes with reduced binding to oviduct cells, resulting in lower fertility rates when AI is performed in the field.…”

Sperm Cohort-Specific Zinc Signature Acquisition and Capacitation-Induced Zinc Flux Regulate Sperm-Oviduct and Sperm-Zona Pellucida Interactions

“…Thus, boar semen extenders are not capable of capitalizing on the fertile aspects of this cohort. Additionally, vanguard cohort-friendly semen supplements or extenders have not been developed, which could increase sperm capability to bind to the oviductal sperm reservoir, especially given that sperm processing techniques, such as sex sorting, produce capacitation-like changes that are detrimental to sperm oviductal reservoir binding [17].…”