Effect of sperm numbers and concentration on sperm transport and uterine inflammatory response in the mare

Fiala, Silvio; Pimentel, Cláudio Alves; Mattos, Ana Luiza Gelpi; Gregory, Ricardo Macedo; Mattos, Rodrigo Costa

doi:10.1016/j.theriogenology.2006.09.005

Cited by 41 publications

(28 citation statements)

References 23 publications

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“…Since either the volume of semen or the total number of spermatozoa used for DIUI were 20 times less than CAI, some seminal plasma proteins that can induce PMN cells might be low. In the mare uterus, the compounds in the insemination dose stimulated a uterine inflammatory response (higher in number of PMN cells), which was more swift and intense as sperm concentration increased [3]. The present study indicated that less inflammatory response was found in the sow oviduct after DIUI and IUI compared to CAI.…”

Section: Discussionsupporting

confidence: 49%

Infiltration of Local Immune Cells in the Sow Reproductive Tracts after Intra-Uterine and Deep Intra-Uterine Insemination with a Reduced Number of Spermatozoa is Less than Conventional Artificial Insemination

Tummaruk

Tienthai

2011

The Journal of Veterinary Medical Science

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ABSTRACT. The present study investigated the infiltration of leukocyte subpopulations in the utero-tubal junction (UTJ) and each part of the oviducts at about 24 hr after intra-uterine insemination (IUI) and deep intra-uterine insemination (DIUI) compared to conventional artificial insemination (CAI) in sows. Fifteen crossbred Landrace x Yorkshire multiparous sows were used (CAI, n=5; IUI, n=5; DIUI, n=5). The sperm dose contained 3,000  10 6 (100 ml), 1,000  10 6 (50 ml) and 150  10 6 (5 ml) motile spermatozoa for CAI, IUI and DIUI, respectively. The sows were inseminated with extended fresh semen at 6 to 8 hr prior to the expected time of ovulation. At 25.2  1.6 hr after insemination, the oviducts and the UTJ were collected. The tissue samples of UTJ, caudal isthmus, cranial isthmus and ampulla were transversely cut to a thickness of 5 m and stained with H&E. The total numbers of lymphocytes, neutrophils, macrophages, eosinophils and plasma cells were determined under light microscope. It was found that the numbers of lymphocytes, eosinophils and macrophages after CAI, IUI and DIUI were not significantly different (P>0.1) in both epithelial and sub-epithelial connective tissue layer of the UTJ, caudal isthmus, cranial isthmus and ampulla. Intra-epithelial neutrophils in the UTJ were higher than cranial isthmus (P<0.05) and ampulla (P<0.05). In the UTJ, the intra-epithelial neutrophil in the CAI group was higher than DIUI group (P<0.01). Plasma cells in sub-epithelial layer of the endosalpinx in the CAI group were higher than DIUI group (P<0.05) and tended to be higher than the IUI group (P=0.08). In conclusion, compared to CAI, IUI and DIUI do not influence the infiltration of lymphocytes, macrophages and eosinophils in the UTJ and the oviduct prior to fertilization. But a lower number of neutrophils in the intra-epithelial layer of the UTJ and plasma cells in the sub-epithelial layers of the oviduct was observed in the DIUI group compared to CAI.KEY WORDS: immune cell, intra-uterine insemination, oviduct, swine.

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Section: Discussionsupporting

confidence: 49%

Infiltration of Local Immune Cells in the Sow Reproductive Tracts after Intra-Uterine and Deep Intra-Uterine Insemination with a Reduced Number of Spermatozoa is Less than Conventional Artificial Insemination

Tummaruk

Tienthai

2011

The Journal of Veterinary Medical Science

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“…Spermatozoa have been shown to be the component in the ejaculate that causes inflammation, with seminal plasma modulating the response , Fiala et al 2007. To account for any differences between the protein composition of the seminal plasma or viability counts between stallions, the seminal plasma was removed and all spermatozoa were killed before insemination.…”

Section: Discussionmentioning

confidence: 99%

Endometrial inflammatory markers of the early immune response in mares susceptible or resistant to persistent breeding-induced endometritis

Woodward¹,

Christoffersen²,

Campos³

et al. 2013

Reproduction

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Transient endometritis after breeding is necessary for clearance of bacteria and spermatozoa; however, in a subpopulation of mares, the inflammation fails to resolve in a timely fashion. The objective of this study was to describe the uterine inflammatory response in mares susceptible or resistant to persistent breeding-induced endometritis (PBIE) during the first 24 h after induction of uterine inflammation. Twelve mares were classified as susceptible (nZ6) or resistant (nZ6) to PBIE. Mares were inseminated over five estrous cycles and endometrial biopsies were collected at one time point per cycle before (0) and 2, 6, 12, and 24 h after insemination. qPCR analysis for IL1B, IL6, IL8, IFNG, TNF (TNFA), IL10, and IL1RN was performed, and endometrial inflammatory cells were counted for each sample. Relative quantification values reported fold changes in mRNA expression from 0 h values. A general pattern of expression post insemination was observed in both groups of mares. Cytokine mRNA increased at 2 h, peaked between 2 and 12 h, and then decreased. Differences were detected between groups of mares 6 h after challenge; resistant mares had higher mRNA expression of IL6, IL1RN, and IL10 than susceptible mares. Susceptible mares had an increased number of polymorphonuclear neutrophils in the endometrium 2 and 12 h after breeding when compared with resistant mares. These findings describe an inherent difference in the initial immune response to insemination and may help explain the transient nature of inflammation in resistant mares, whereas susceptible mares develop a persistent inflammation.

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“…The characteristics and grade of endometrial inflammation may vary depending on the sperm number and concentration, seminal plasma, semen extender and number of insemination [6,7] . If mares are inseminated twice with an interval of 24 h, sperm in the second inseminate induce directly an inflammatory response.…”

Section: Introductionmentioning

confidence: 99%

Kısraklarda Tek ve Çift Taze Sperm ile Tohumlama Sonrası Uterin İmmun Yanıt

Gündüz

Dal

Kurban

et al. 2017

Kafkas Univ Vet Fak Derg

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The aim of the study was to evaluate the effects of single and double inseminations on the inflammation of the uterus in mares. Nine mix breed mares with endometrial biopsy scores grade I and IIA, aged 5-15, were used in the study. Two experiments were performed over two estrous cycles: (1) single insemination, (2) double insemination. All mares were used in both insemination group. In the single insemination cycles one insemination was performed 24 h after hCG injection, while in the double insemination cycles two inseminations were carried out 24 h and 48 h after the hCG injection. Endometrial biopsies were collected in both cycles immediately before (=0), and 6, 30 and 54 h after the first insemination. Gene expressions of the inflammatory markers IL-1β, IL-6, IL-8, iNOS, SAA, COX-2 and CASP-3 and histopathological alterations of the endometrium were determined. The 6 h expression levels of IL-6 were higher when compared with 30 h and 54 h (P<0.05). CASP-3 expression levels in single insemination cycles were higher at 0 h and 6 h when compared with 54 h (P<0.05). In conclusion expression levels of the IL-1β,-6,-8, iNOS, SAA and COX-2 were not different between single or double insemination cycles. This study provides preliminary evidence to further characterize the changes in the expression of relevant genes in response to fresh semen. Keywords: Mare, Postbreeding endometritis, Proinflammatory cytokines, Insemination Kısraklarda Tek ve Çift Taze Sperm İle Tohumlama Sonrası Uterin İmmun Yanıt ÖzetÇalışmada kısraklarda tek ve çift tohumlama sonrası immun yanıt değerlendirildi. Farklı ırktan, 5-15 yaşlarında ve endometriyal biyopsi skoru I ve IIA olan 9 adet kısrak kullanıldı. İki östrus siklusunda iki uygulama yapıldı: (1) tek tohumlama (2) çift tohumlama. Herbir kısrak tek ve çift tohumlama olmak üzere iki deney grubunda da yer aldı. Tekli uygulamalarda hCG uygulaması sonrası 24. saatte tohumlama yapılıyorken; çiftli uygulamalarda hCG uygulaması sonrası 24. ve 48. saatte tohumlama yapıldı. Tohumlamadan hemen önce (0.) ve tohumlamadan sonraki 6., 30. ve 54. saatte uterus biyopsi örnekleri alındı. Yangı parametrelerinden IL-1β, IL-6, IL-8, iNOS, SAA, COX-2 ve CASP-3 mRNA ekspresyonları ve endometriumdaki histopatolojik değişimler değerlendirildi. Tekli gruptaki kısraklarda 6. saatdeki IL-6 mRNA expresyonları 30. ve 54. saatlerdeki değerlere göre yüksek bulundu (P<0.05). 0. ve 6. saatlerdeki CASP-3 ekspresyonları ise 54. saatdeki değerlere göre yüksek bulundu (P<0.05). Sonuç olarak tekli ve çiftli gruplar arasında IL-1β,-6,-8, iNOS, SAA ve COX-2 expresyonları açısından fark bulunmadı. Taze semen ile yapılan tohumlamalardan sonra bahsi geçen gen expresyonlarına bakarak immun yanıt hakkında ön fikir elde edilebileceği anlaşıldı.

show abstract

Effect of sperm numbers and concentration on sperm transport and uterine inflammatory response in the mare

Cited by 41 publications

References 23 publications

Infiltration of Local Immune Cells in the Sow Reproductive Tracts after Intra-Uterine and Deep Intra-Uterine Insemination with a Reduced Number of Spermatozoa is Less than Conventional Artificial Insemination

Infiltration of Local Immune Cells in the Sow Reproductive Tracts after Intra-Uterine and Deep Intra-Uterine Insemination with a Reduced Number of Spermatozoa is Less than Conventional Artificial Insemination

Endometrial inflammatory markers of the early immune response in mares susceptible or resistant to persistent breeding-induced endometritis

Kısraklarda Tek ve Çift Taze Sperm ile Tohumlama Sonrası Uterin İmmun Yanıt

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