Effect of static magnetic fields and phloretin on antioxidant defense system of human fibroblasts

Pawłowska-Góral, Katarzyna; Kimsa-Dudek, Magdalena; Synowiec-Wojtarowicz, Agnieszka; Orchel, Joanna; Glinka, Marek; Gawron, S.

doi:10.1007/s11356-016-6653-x

Cited by 14 publications

(13 citation statements)

References 32 publications

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“…The available evidence from in vitro and in vivo studies is considered inadequate to decide about the potential impact of SMF exposure because contradictory results were reported regarding the effect of SMF on antioxidant enzymes and oxidative stress. Additionally, the mechanism of action which SMFs exert on cells still remains undisclosed [22]. …”

Section: Discussionmentioning

confidence: 99%

Effect of Static Magnetic Field on Oxidant/Antioxidant Parameters in Cancerous and Noncancerous Human Gastric Tissues

Öztürk

Durak²,

Büber

et al. 2016

Scientifica

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Aim. To investigate the effects of static magnetic field (SMF) on oxidant and antioxidant parameters of the cancerous and noncancerous human gastric tissues. Materials and Methods. Gastric tissues obtained from patients with gastric cancer were used in the study. SMF was created by using two static magnets. Before and after treatment with SMF, oxidant and antioxidant parameters were measured in the tissue samples. Results. In the cancerous tissue, superoxide dismutase (SOD) activity was found higher and malondialdehyde (MDA) level was found lower as compared with noncancerous tissue. SMF affects oxidant/antioxidant parameters differently in the cancerous and noncancerous tissues. In this regard, SMF causes increase in SOD activity and decrease in MDA level in the noncancerous tissue. However, it decreases SOD and glutathione peroxidase (GSH-Px) activities and increases MDA level and catalase (CAT) activity in the cancerous tissue. There were no differences between nitric oxide (NO) and nitric oxide synthase (NOS) parameters in or among the cancerous and noncancerous tissues. Conclusions. SMF accelerates peroxidation reactions possibly by suppressing SOD and GSH-Px enzymes in the cancerous gastric tissue. This event caused by SMF might play part in the death of cancer cells, which may be a good supportive vehicle for the cancer therapy.

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Section: Discussionmentioning

confidence: 99%

Effect of Static Magnetic Field on Oxidant/Antioxidant Parameters in Cancerous and Noncancerous Human Gastric Tissues

Öztürk

Durak²,

Büber

et al. 2016

Scientifica

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“…In general, PH has high safety margin with less side effects. Several previous in vitro and in vivo studies showed that PH is not toxic to several non-cancerous cells such as epithelial breast cells MCF10A [18] and normal human dermal fibroblast [19]. It has also been reported that PH scavenges ONOO- and inhibits lipid peroxidation in rat liver microsomes [20].…”

Section: Introductionmentioning

confidence: 99%

Phloretin attenuates STAT-3 activity and overcomes sorafenib resistance targeting SHP-1–mediated inhibition of STAT3 and Akt/VEGFR2 pathway in hepatocellular carcinoma

et al. 2019

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BackgroundHepatocellular carcinoma (HCC) is the most common primary liver malignancy. Phloretin (PH) possesses anticancer, antitumor, and hepatoprotective effects, however, the effects and potential mechanisms of phloretin remain elusive.MethodsFive HCC cells were tested in vitro for sensitivity to PH, Sorafenib (Sor) or both and the apoptosis, signal transduction and phosphatase activity were analyzed. To validate the role of SHP-1, we used PTP inhibitor III and SHP-1 siRNA. Further, we used purified SHP-1 proteins or HCC cells expressing deletion N-SH2 domain or D61A point mutants to study the PH efficacy on SHP-1. The `in vivo studies were conducted using HepG2 and SK-Hep1 and Sor resistant HepG2SR and Huh7SR xenografts. Molecular docking was done with Swiss dock and Auto Dock Vina.ResultsPH inhibited cell growth and induced apoptosis in all HCC cells by upregulating SHP-1 expression and downregulating STAT3 expression and further inhibited pAKT/pERK signaling. PH activated SHP-1 by disruption of autoinhibition of SHP-1, leading to reduced p-STAT3Tyr705 level. PH induced apoptosis in two Sor-resistant cell lines and overcome STAT3, AKT, MAPK and VEGFR2 dependent Sor resistance in HCCs. PH potently inhibited tumor growth in both Sor-sensitive and Sor-resistant xenografts in vivo by impairing angiogenesis, cell proliferation and inducing apoptosis via targeting the SHP-1/STAT3 signaling pathway.ConclusionOur data suggest that PH inhibits STAT3 activity in Sor-sensitive and -resistant HCCs via SHP-1–mediated inhibition of STAT3 and AKT/mTOR/JAK2/VEGFR2 pathway. Our results clearly indicate that PH may be a potent reagent for hepatocellular carcinoma and a noveltargeted therapy for further clinical investigations.Graphical abstract Electronic supplementary materialThe online version of this article (10.1186/s12964-019-0430-7) contains supplementary material, which is available to authorized users.

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“…The viability of the cells was assessed after 24 hr and 48 hr of CGA treatment. Two experiments were performed as was previously reported (Pawłowska‐Góral et al., 2016).…”

Section: Methodsmentioning

confidence: 99%

The impact of the co‐exposure of melanoma cells to chlorogenic acid and a moderate‐strength static magnetic field

et al. 2020

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One of the most malignant skin cancers is melanoma-it has a high metastatic potential as well as a high resistance to treatment, which results in a high mortality rate. The basic method for treating early-stage melanomas is surgery alone. If surgery is not possible or as an adjuvant therapy, radiotherapy is used; chemotherapy is rarely used because of its low response rate (Perera et al., 2014). One promising therapeutic strategy is targeted therapy that involves blocking the BRAF kinase (about 50% of patients have a BRAF mutation), which results in a disruption of the MAPK signaling pathway and slows down the proliferation of cancer cells (Zaman et al., 2019). In addition, research is being conducted into the use of antimelanoma vaccines (Vasquez et al., 2017). Nonetheless, the low level of the effectiveness of these treatments, especially in the 3rd and 4th stages of its clinical advancement, has led to a search for new

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Effect of static magnetic fields and phloretin on antioxidant defense system of human fibroblasts

Cited by 14 publications

References 32 publications

Effect of Static Magnetic Field on Oxidant/Antioxidant Parameters in Cancerous and Noncancerous Human Gastric Tissues

Effect of Static Magnetic Field on Oxidant/Antioxidant Parameters in Cancerous and Noncancerous Human Gastric Tissues

Phloretin attenuates STAT-3 activity and overcomes sorafenib resistance targeting SHP-1–mediated inhibition of STAT3 and Akt/VEGFR2 pathway in hepatocellular carcinoma

The impact of the co‐exposure of melanoma cells to chlorogenic acid and a moderate‐strength static magnetic field

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