Effect of storage temperature on sperm cryopreservation

Trummer, Harald; Tucker, Kathleen; Young, Cara Capitena; Kaula, Norbert; Meacham, Randall B.

doi:10.1016/s0015-0282(98)00349-5

Cited by 27 publications

(13 citation statements)

References 11 publications

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“…This suggests that a storage temperature of À179 C has no effect on sperm morphology compared with storage at À196 C. These results are consistent with those of studies elsewhere (9,10,22).…”

Section: Discussionsupporting

confidence: 92%

“…The study of Trummer et al (22) found greater decreases in sperm motility recovery in specimens stored at À70 C, compared with those stored at À196 C (47% vs. 39%), and the loss of sperm motility increased dramatically after long-term storage. This may be explained by the effect of higher storage temperature (by À70 C) on postthaw sperm motility.…”

Section: Discussionmentioning

confidence: 94%

“…This may be explained by the effect of higher storage temperature (by À70 C) on postthaw sperm motility. Subsequent to the Trummer et al (22) work, the study of Saritha and Bongso (9) showed greater recovery of sperm motility after storage in vapor at À189 C for 3 days. Those investigators commented that this may have been attributable to lesser fluctuations in temperature between the vapor phase at À189 C and the liquid phase at À196 C. However, at the same storage temperature (À189 C) in the vapor phase of liquid nitrogen, Amesse et al (10) reported no significant difference in postthaw motility recovery compared with the case of storage in liquid nitrogen.…”

Section: Discussionmentioning

confidence: 95%

See 2 more Smart Citations

Comparison of cryopreserved human sperm in vapor and liquid phases of liquid nitrogen: effect on motility parameters, morphology, and sperm function

Punyatanasakchai

Sophonsritsuk

Weerakiet

et al. 2008

Fertility and Sterility

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Comparison of cryopreserved human sperm in vapor and liquid phases of liquid nitrogen: effect on motility parameters, morphology, and sperm function

Punyatanasakchai

Sophonsritsuk

Weerakiet

et al. 2008

Fertility and Sterility

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“…The latter is associated with a greater decrease in motility after 7 days of storage. This difference is accentuated following 3 months of storage; although no differences in sperm morphology is observed [Trummer et al 1998]. Freezing can be detrimental to the cell because of osmotic imbalances and ice crystal formation formed at different rates during the cooling process.…”

Section: Introductionmentioning

confidence: 99%

Effects of Cryopreservation on Sperm DNA Integrity in Normospermic and Four Categories of Infertile Males

Ahmad

Jalali

Shami

et al. 2010

Systems Biology in Reproductive Medicine

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This study examined the effects of cryopreservation on DNA integrity of spermatozoa from 34 fertile subjects and 166 infertile subjects comprised of 80 teratospermic, 32 normospermic, 30 astheno-teratospermic, and 24 oligo-astheno-teratospermic individuals. Semen samples were prepared by swim-up and the Percoll density gradient centrifugation method (Pdgc) prior to freezing in liquid nitrogen. Neat and prepared samples were supplemented with cryoprotectant (SpermFreez) in cryoampoules and were frozen using the static phase vapor cooling procedure. Sperm DNA integrity of all thawed samples was determined using the alkaline comet assay. It was noticed that the sperm DNA integrity of frozen samples of fertile subjects was considerably higher than that of infertile subjects with greater catch-up integrity similar to the fresh samples. Freezing caused less chromatin damage to sperm of Pdgc samples from both fertile and infertile subjects as was compared to the neat and swim-up samples. It is concluded that the increase in comet frequency of frozen-thawed samples from infertile subjects was more prominent (8.25-22.78%; P50.01) than in the fresh samples. Frozen-thawed samples from Ts (Teratospermic individuals) and ATs (Astheno-teratozoosspermic) showed higher level of OTM (Olive tail moment) indicating a higher level of chromatin fragmentation than fertile, Ns (Normospermics), and OATs (Oligo-astheno-teratozoospermics).

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“…In the last decades, research has focused on the improvement of biotechnology for animal breeding and methods of semen preservation for artificial insemination, embryo transfer and in vitro fertilization (Trummer et al, 1998;Holt, 2000;Yoshida, 2000;Pauw et al, 2003). In this 7…”

mentioning

confidence: 99%

Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen

Aguiar

Tilburg

Catunda

et al. 2013

Arq. Bras. Med. Vet. Zootec.

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Effect of storage temperature on sperm cryopreservation

Cited by 27 publications

References 11 publications

Comparison of cryopreserved human sperm in vapor and liquid phases of liquid nitrogen: effect on motility parameters, morphology, and sperm function

Comparison of cryopreserved human sperm in vapor and liquid phases of liquid nitrogen: effect on motility parameters, morphology, and sperm function

Effects of Cryopreservation on Sperm DNA Integrity in Normospermic and Four Categories of Infertile Males

Sperm parameters and biochemical components of goat seminal plasma in the rainy and dry seasons in the Brazilian Northeast: the season's influence on the cooling of semen

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