Effect of Storage Time and Temperature on Salivary Total Antioxidant Capacity, Total Oxidant Status, and Oxidant Stress Index

Abstract

Objective The aim of this study was to compare the effects of short-term storage in two different temperatures on stability of salivary total antioxidant capacity (TAC), total oxidant status (TOS) and oxidant stress index (OSI). Materials and methods Saliva samples were collected from twenty healthy volunteers for the study. An aliquot was selected for immediate analysis and the rest was stored at -20°C and -80°C for a period of 120 days, and analyzes were performed at 15, 30, 45, 60, 90 and 120 days. The determination of the TOS and the TAC were performed by colorimetric methods. Results The results show that the two storage temperatures were able to preserve oxidants and antioxidants up to 60 days with similar levels when compared with fresh samples. When comparing the different storage temperatures at each time point, no significant differences were observed. Finally, the OSI remains constant over time of storage at both temperatures without statistically significant differences between them. The results were expressed as mean ± standard error of mean. Statistical analysis was performed using the repeated-measures analysis of variance (ANOVA), the Bonferroni and t-test. A p-value <0.05 was accepted to be statistically significant. Conclusion The two temperatures were able to keep salivary TOS and TAC levels similar to fresh saliva samples. Therefore they are reliable for assessing oxidative stress up to 60 days.

Variability of Saliva Viscosity - Potential Impact

“…Bansil et al [14] provide a molecular interpretation on how the structure of mucin leads to entanglements among the biopolymers in solution and create a range of viscosity effects depending upon concentration and mucin type. They suggest that a dilute solution of mucin generally has viscoelastic behavior that depends upon shear rate.…”

Profiling salivary miRNA expression levels in Fanconi anemia patients – a pilot study

“…Participants were asked to refrain from eating, drinking or brushing their teeth for at least one hour prior to saliva sample collection. All samples were collected in the morning between 7:30 am to 11:30 am to minimize diurinal variations [ 24 , 26 – 28 ]. In brief, participants were asked to refrain from eating 1-h prior to saliva collection.…”

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“…U dosadašnjim studijama autori su istaknuli da toplinsko i mehaničko opterećenje značajno utječe na mehanička svojstva materijala alignera te rezultira promjenama u tvrdoći, elastičnosti i čvrstoći (13, 17 -19). Također upozoravaju da te naprave mogu doživjeti promjene u obliku i sastavu u oralnome okruženju zbog čimbenika kao što su temperatura, vlažnost i salivarni enzimi (10,20,21). Razumijevanje mehaničkog ponašanja alignera pod različitim stresom ključno je za optimizaciju njihovih performansi.…”

“…Previous studies have shown that thermal and mechanical loading significantly affect the mechanical properties of aligner materials, thus leading to changes in hardness, elasticity, and strength (13,(17)(18)(19). Also, studies have indicated that aligners may undergo changes in shape and composition in the oral environment due to factors such as temperature, humidity, and salivary enzymes (10,20,21). Understanding the mechanical behavior of aligners under various stresses is essential for optimizing their performance.…”