Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows

Abstract

The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at day 7 of the estrous cycle to start the SOV protocol, as well as on the day of embryo recovery (DER). The SOV group produced 7.5 ± 6.7 oocytes/embryos, of which 3.4 ± 4.7 were TE. Serum urea and E2 and uterine Glu, CK, LDH, TP, P4 and PGFM in the control group and serum P4 and PGFM and uterine LDH and PGFM in the SOV group were significantly higher (p < 0.01) at DER than day 7. At DER, uterine urea, LDH, PGFM and TP and serum urea, LDH, PGFM, and P4 concentrations were higher (p < 0.01) in the SOV group than the control. There was no significant variation in uterine bacteriology or cytology. Overall, these results infer that SOV affects both serum profile and uterine secretions, and that these changes may influence the number of TE.

Velvet Antler Production and Hematological Changes in Male Sika Deers Fed with Spent Mushroom Substrate

“…Serum BUN concentration has an important relationship with nitrogen metabolism, which can reflect the ratio of protein and non-protein nitrogen levels in ruminant diets [28]. High levels of protein in the diet raise serum ammonia levels and disrupt the immune system of dairy cows [29]. As the results showed, the content of BUN tended to decrease in animals fed SMS, but there was no significant difference.…”

Influence of breed on the quality of in vivo produced embryos from Boran and Holstein Friesian cross dairy breed in Ethiopia

“…In line with a report by Degefa et al (2018) on similar animals, the Boran breed showed a comparable ovarian response as the HF*Boran, however, recovery of UFO was much higher in Boran cows compared to the HF*Boran crosses. A likely cause is fertilization failures were reported by several authors (Sartori et al, 2010;Rasolomboahanginjatovo et al, 2014;Dorice et al, 2019) where a higher proportion of non-fertilized oocytes and degenerated embryos have been frequently recovered. Fertilization failure in superovulated cattle is generally more pronounced, averaging approximately 45%, much lower than the present finding.…”

Association between metabolic parameters and embryo production in superovulated dairy cattle

“…(2021) and Rasolomboahanginjatovo et al. (2014) in terms of plasma glucose levels and daily changes of plasma glucose levels. In the research, the interaction of plasma glucose level with groups and days was not found statistically significant, and no significant correlation was found with the number of embryos.…”

“…On the other hand, Snider et al (2021) determined that serum glucose levels did not change during superovulation. Interestingly, Rasolomboahanginjatovo et al (2014) compared serum and uterine fluid glucose levels on different days with samples taken from donors treated with and without superovulation. There was no difference between the superovulated and non-superovulated groups.…”

“…In this study, plasma glucose levels were found to be within or very close to the values accepted as normal for cattle (Animal Health Diagnostic Center, 2023). Furthermore, this study is in parallel with Snider et al (2021) and Rasolomboahanginjatovo et al (2014) in terms of plasma glucose levels and daily changes of plasma glucose levels. In the research, the interaction of plasma glucose level with groups and days was not found statistically significant, and no significant correlation was found with the number of embryos.…”