Effect of surface modification on protein retention and cell proliferation under strain

Dunkers, Joy P.; Lee, H.-J.; Matos, Marvi A.; Pakstis, Lisa; Taboas, Juan M.; Hudson, Steven D.; Cicerone, Marcus T.

doi:10.1016/j.actbio.2011.04.005

Cited by 5 publications

(2 citation statements)

References 27 publications

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“…The most commonly used techniques include chemical wear, plasma treatment and exposure to gamma radiation. Of these techniques, plasma treatment is particularly versatile because the modification is restricted to the surface without compromising the material properties as a whole [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Polymer films with surfaces unmodified and modified by non-thermal plasma as new substrates for cell adhesion

Borges

Benetoli

Licínio

et al. 2013

Materials Science and Engineering: C

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Section: Introductionmentioning

confidence: 99%

Polymer films with surfaces unmodified and modified by non-thermal plasma as new substrates for cell adhesion

Borges

Benetoli

Licínio

et al. 2013

Materials Science and Engineering: C

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“…Most cells, including VSMCs, will not adhere to unmodified PDMS. Several surface modification techniques have been proposed to effectively 'mask' biological materials from the hydrophobicity of PDMS [21][22][23][24]. However, following these treatments, cells are rarely viable past one week.…”

Section: Introductionmentioning

confidence: 99%

Long-term vascular contractility assay using genipin-modified muscular thin films

et al. 2014

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Vascular disease is a leading cause of death globally and typically manifests chronically due to long-term maladaptive arterial growth and remodeling. To date, there is no in vitro technique for studying vascular function over relevant disease time courses that both mimics in vivo-like tissue structure and provides a simple readout of tissue stress. We aimed to extend tissue viability in our muscular thin film contractility assay by modifying the polydimethylsiloxane (PDMS) substrate with micropatterned genipin, allowing extracellular matrix turnover without cell loss. To achieve this, we developed a microfluidic delivery system to pattern genipin and extracellular matrix proteins on PDMS prior to cell seeding. Tissues constructed using this method showed improved viability and maintenance of in vivo-like lamellar structure. Functional contractility of tissues fabricated on genipin-modified substrates remained consistent throughout two weeks in culture. These results suggest that muscular thin films with genipin-modified PDMS substrates are a viable method for conducting functional studies of arterial growth and remodeling in vascular diseases.

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Bio/non-bio interfaces: A straightforward method for obtaining long term PDMS/muscle cell biohybrid constructs

Genchi

Ciofani

Liakos

et al. 2013

Colloids and Surfaces B: Biointerfaces

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Effect of surface modification on protein retention and cell proliferation under strain

Cited by 5 publications

References 27 publications

Polymer films with surfaces unmodified and modified by non-thermal plasma as new substrates for cell adhesion

Polymer films with surfaces unmodified and modified by non-thermal plasma as new substrates for cell adhesion

Long-term vascular contractility assay using genipin-modified muscular thin films

Bio/non-bio interfaces: A straightforward method for obtaining long term PDMS/muscle cell biohybrid constructs

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