Effect of Surface Treatment on Diffusion and Domain Formation in Supported Lipid Bilayers

Seu, Kalani J.; Pandey, Anjan P.; Haque, Farzin; Proctor, Elizabeth A.; Ribbe, Alexander E.; Hovis, Jennifer S.

doi:10.1529/biophysj.106.099721

Cited by 177 publications

(181 citation statements)

References 36 publications

Supporting

Mentioning

169

Contrasting

Unclassified

Order By: Relevance

“…Also, self-diffusion coefficients fitted using Soumpasis's solution agree qualitatively with our reaction-diffusion FRAP model (figure 6), indicating that the qualitative differences in the three studies are more likely due to the substrate cleaning procedures. However, in contrast to the observations of Seu et al [66], lipopolymer self-diffusion coefficients in our study (system I) on piranha etched glasses are actually smaller than the values from system II using baked glasses. At present, it seems that every parameter plays a role in the dynamics of the three systems, and that the principal contribution remains elusive.…”

Section: Comparison With Literaturecontrasting

confidence: 99%

Concentration dependence of lipopolymer self-diffusion in supported bilayer membranes

Hill

2010

J. R. Soc. Interface.

View full text Add to dashboard Cite

Self-diffusion coefficients of poly(ethylene glycol)2k-derivatized lipids (DSPE-PEG2k-CF) in glass-supported DOPC phospholipid bilayers are ascertained from quantitative fluorescence recovery after photobleaching (FRAP). We developed a first-order reaction -diffusion model to ascertain the bleaching constant, mobile fraction and lipopolymer self-diffusion coefficient D s at concentrations in the range c % 0.5 -5 mol%. In contrast to control experiments with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (ammonium salt) (DOPE-NBD) in 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the lipopolymer self-diffusion coefficient decreases monotonically with increasing concentration, without a distinguishing mushroom-to-brush transition. Our data yield a correlation, where D 0 % 3.36 mm 2 s 21 and a % 0.56 (with c expressed as a mole percent). Interpreting the dilute limit with the Scalettar-Abney -Owicki statistical mechanical theory for transmembrane proteins yields an effective disc radius a e % 2.41 nm. On the other hand, the Bussell -Koch-Hammer theory, which includes hydrodynamic interactions, yields a e % 2.92 nm. As expected, both measures are smaller than the Flory radius of the 2 kDa poly(ethylene glycol) (PEG) chains, R F % 3.83 nm, and significantly larger than the nominal radius of the phospholipid heads, a l % 0.46 nm. The diffusion coefficient at infinite dilution D 0 was interpreted using the Evans-Sackmann theory, furnishing an inter-leaflet frictional drag coefficient b s % 1.33 Â 10 8 N s m 23. Our results suggest that lipopolymer interactions are dominated by the excluded volume of the PEG-chain segments, with frictional drag dominated by the two-dimensional bilayer hydrodynamics.

show abstract

Section: Comparison With Literaturecontrasting

confidence: 99%

Concentration dependence of lipopolymer self-diffusion in supported bilayer membranes

Hill

2010

J. R. Soc. Interface.

View full text Add to dashboard Cite

show abstract

“…In order for the surface modification to inhibit cell adhesion, the substrate used must be through a cleaning protocol first by immersed in a piranha solution [16,1,17,18,15,26,19]. The silicon substrate need to be cleaned to get rid of the contaminates before the substrate was ready for the coating process.…”

Section: Principles Of Surface Functionalizationmentioning

confidence: 99%

“…The purposes of using AFM are to identify the surface topography [18,16] and determined the surface roughness by measuring the root mean square roughness (R rms ) parameter [18,1]. All images were collected in tapping mode [16,26,18] in air at room temperature and underwater condition [26] using low-force silicon scanning probe [16,1,18] and using optical beam deflection [1].…”

Section: Principles Of Surface Functionalizationmentioning

confidence: 99%

See 1 more Smart Citation

The study of non-fouling and non-specific cellular binding on functionalized surface for mammalian cell identification and manipulation

Zainudin¹,

Hambali²,

Wahid³

et al. 2017

AIP Conference Proceedings

View full text Add to dashboard Cite

Signal characteristics by optimization the output coupling ratio of multi-wavelength Brillouin fiber laser incorporating fiber Bragg grating in a ring cavity technique AIP Conference Proceedings 1835, 020002 (2017) Abstract.Surface functionalization has emerged as a powerful tool for mapping limitless surface-cell membrane interaction in diverse biomolecular applications. Inhibition of non-specific biomolecular and cellular adhesion to solid surfaces is critical in improving the performance of some biomedical devices, particularly for in vitro bioassays. Some factors have to be paid particular attention in determining the right surface modification which are the types of surface, the methods and chemical solution that being used during the experimentation and also tools for analyzing the results. Improved surface functionalization technologies that provide better non-fouling performance in conjunction with specific attachment chemistries are sought for these applications. Hence, this paper serves as a review for multiple surface treatment methods including PEG grafting, adsorptive chemistries, self-assembled monolayers (SAMs) and plasma treatments.

show abstract

“…To make the surface hydrophilic, it is necessary to hydroxylate the surface, breaking the siloxane bonds and creating silanol groups (with structure Si-O-H) on the glass surface [135]. Piranha solution is a powerful oxidiser, capable of hydroxylating the glass surface and creating hydrophilic silanol groups on the glass surface.…”

Section: Sample Preparationmentioning

confidence: 99%

Nitrogen-vacancy centres in diamond: internal dynamics and nanoscale measurements

Chapman¹

View full text Add to dashboard Cite

This thesis represents several steps in the development of Nitrogen-Vacancy Nanodiamond as an effective optical label for biological measurements and as a tool for nanometry and quantum information techniques. In this thesis I cover concrete progress in many areas of nanodiamond research, demonstrating that optical interactions alone can be used to control NV spin polarisation and measure NV temperature. I develop a method for high-contrast imaging of NV centres using magnetic modulation, and also show a method for measuring the Raman scattering of water to an unprecedented level of accuracy. This thesis also contains the outlines of two more experiments: one aimed at measuring NV concentration in aqueous nanodiamond suspensions, and another whose aim is to use multiple NV centres in a single nanodiamond to measure the orientation and strength of an external magnetic field.iii

show abstract

Effect of Surface Treatment on Diffusion and Domain Formation in Supported Lipid Bilayers

Cited by 177 publications

References 36 publications

Concentration dependence of lipopolymer self-diffusion in supported bilayer membranes

Concentration dependence of lipopolymer self-diffusion in supported bilayer membranes

The study of non-fouling and non-specific cellular binding on functionalized surface for mammalian cell identification and manipulation

Nitrogen-vacancy centres in diamond: internal dynamics and nanoscale measurements

Contact Info

Product

Resources

About