Effect of system variables involved in packed column supercritical fluid chromatography of stavudine taken as model analyte using response surface methodology along with study of thermodynamic parameters

Kaul, Neeraj; Agrawal, Himani; Paradkar, Anant; Mahadik, Kakasaheb R.

doi:10.1016/j.jpba.2006.07.038

Cited by 13 publications

(5 citation statements)

References 21 publications

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“…The contribution was negative, which means that increasing the methanol fraction decreased the retention factors. The quadratic dependence of the methanol coefficient, C m 2 , has previously been demonstrated in both chiral [17,22] and achiral SFC [37,38]. The effect of C m 2 on the retention models (k 1 , k 2 ) is positive, indicating increased retention ( Table 2).…”

Section: Normalized Coefficients In Retention Factor Modelsmentioning

confidence: 67%

“…Retention factors for the first-and second-eluting enantiomer (k 1 and k 2 ) and selectivity (␣) were responses in the models. Empirical observations suggest a logarithmic relationship between the retention factor and temperature/co-solvent fraction [22,[35][36][37][38][39]. The models were refined by removing statistical outliers and parameters that were statistically insignificant at a 95% confidence level.…”

Section: Discussionmentioning

confidence: 99%

“…A full-factorial design in three levels with three center points (30 runs in total) was selected because: (i) we expect the retention factor to have a quadratic relationship with the methanol fraction, which leads to the need for a design that can fit quadratic terms [22,[35][36][37][38], and (ii) interaction terms are expected. The latter was observed for the chiral separation of (±)-1,1 -Bi-2-naphthol and (±)-trans-stilbene oxide on a Kromasil Cellucoat column [26].…”

Section: Design Of Experimentsmentioning

confidence: 99%

See 2 more Smart Citations

Chemometric evaluation of the combined effect of temperature, pressure, and co-solvent fractions on the chiral separation of basic pharmaceuticals using actual vs set operational conditions

Forss

Haupt

Stålberg

et al. 2017

Journal of Chromatography A

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Section: Normalized Coefficients In Retention Factor Modelsmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Design Of Experimentsmentioning

confidence: 99%

See 1 more Smart Citation

Chemometric evaluation of the combined effect of temperature, pressure, and co-solvent fractions on the chiral separation of basic pharmaceuticals using actual vs set operational conditions

Forss

Haupt

Stålberg

et al. 2017

Journal of Chromatography A

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“…PGC has excellent thermal and pH stability enabling the use of a wide range of chromatographic conditions. The chromatographic behavior of the PGC stationary phase was described by West et al [185]. Due to the specific interactions, PGC columns were used primarily for the separation of isomers and compounds with closely similar structures, for example, isosorbide-5-mononitrate [186], aromatic isomers [187], metoprolol and related amino alcohols [188], plasticizers [189][190][191], plant diterpene esters [192], flower fingerprinting [193], nitrosamines [194,195], and essential oils [196].…”

Section: Unconventional Stationary Phases In Sfcmentioning

confidence: 99%

Columns in analytical‐scale supercritical fluid chromatography: From traditional to unconventional chemistries

Plachká

Pilařová

Horáček

et al. 2023

J of Separation Science

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Within this review, we thoroughly explored supercritical fluid chromatography (SFC) columns used across > 3000 papers published from the first study carried out under SFC conditions in 1962 to the end of 2022. We focused on the open tubular capillary, packed capillary, and packed columns, their chemistries, dimensions, and trends in used stationary phases with correlation to their specific interactions, advantages, drawbacks, used instrumentation, and application field. Since the 1990s, packed columns with liquid chromatography and SFC‐dedicated stationary phases for chiral and achiral separation are predominantly used. These stationary phases are based on silica support modified with a wide range of chemical moieties. Moreover, numerous unconventional stationary phases were evaluated, including porous graphitic carbon, titania, zirconia, alumina, liquid crystals, and ionic liquids. The applications of unconventional stationary phases are described in detail as they bring essential findings required for further development of the supercritical fluid chromatography technique.

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“…peak position, particle size in column, viscosity of mobile phase and molecular weight of analyte [24,25].…”

Section: Theoretical Plates (N)mentioning

confidence: 99%

Response surface based optimization of system variables for liquid chromatographic analysis of candesartan cilexetil

Manwar

Vispute

Kumbhar

et al. 2017

Journal of Taibah University for Science

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A statistical optimization method was successfully employed to study the effect of system variables on the chromatographic analysis of candesartan cilexetil. The effect of simultaneously varying the flow rate, temperature and concentration of acetonitrile in the mobile phase in water (0.05% O-phosphoric acid (0.05% OPA)) was studied to optimize the method to obtain excellent chromatographic responses. The optimum conditions were determined with the help of response surface methodology (RSM) using Plackett-Burman designs. From the response surface graphs, the optimum regions were selected to be −1, +1 and +1 for flow rate (0.8 ml/min), temperature (25 • C) and concentration of acetonitrile in water (0.05% OPA) (80%, v/v), respectively. Pareto ranking indicated that the most important variable affecting the selected responses was temperature. Linearity was found in the range of 10 of 50 g/ml, with a significantly high correlation coefficient (r 2 = 0.9989). The limits of detection and quantitation were 0.12 and 0.33 g/ml, respectively. The developed method was validated for accuracy, precision, linearity, range, and specificity. The method was successfully used to analyze a tablet formulation to assess the chromatographic performance, and it was found to be 99.03%, with a standard deviation of ±0.04.

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Effect of system variables involved in packed column supercritical fluid chromatography of stavudine taken as model analyte using response surface methodology along with study of thermodynamic parameters

Cited by 13 publications

References 21 publications

Chemometric evaluation of the combined effect of temperature, pressure, and co-solvent fractions on the chiral separation of basic pharmaceuticals using actual vs set operational conditions

Chemometric evaluation of the combined effect of temperature, pressure, and co-solvent fractions on the chiral separation of basic pharmaceuticals using actual vs set operational conditions

Columns in analytical‐scale supercritical fluid chromatography: From traditional to unconventional chemistries

Response surface based optimization of system variables for liquid chromatographic analysis of candesartan cilexetil

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