Effect of Temporary Abdominal Closure on Colonic Anastomosis and Postoperative Adhesions in Experimental Secondary Peritonitis

Aydın, Çiğdem; Aytekin, Faruk Önder; Tekin, Koray; Kabay, Burhan; Yenisey, Çiğdem; Kocbil, Goksel

doi:10.1007/s00268-005-0511-1

Cited by 7 publications

(5 citation statements)

References 61 publications

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“…Collagen is important at all stages of wound healing and is critical to the overall strength and integrity of the tissue. However, the final anastomotic strength is not always related to the absolute concentration of collagen, the structure and arrangement of the collagen matrix may be more important 20 .…”

Section: Discussionmentioning

confidence: 99%

Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

Silva¹,

Carneiro

Oliveira

et al. 2012

Acta Cir. Bras.

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PuRPOSE:To evaluate the effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis. METHOdS: Forty rats were divided into two groups to receive either bromopride (experimental group-E) or saline (control group-C). Each group was divided into subgroups of ten animals each to be euthanized on third (E3 and C3) or seventh day (E7 and C7) after surgery. Sepsis was induced by cecal ligation and puncture. The rats underwent segmental left colon resection and end-to-end anastomosis. Adhesion formation, tensile strength and hydroxyproline concentration were assessed. Histomorphometry of collagen and histopathological analysis were also performed. RESuLTS: On postoperative third day, anastomoses in bromopride-treated animals showed lower tensile strength (p=0.02) and greater reduction in hydroxyproline concentration (p=0.04) than in control animals. There was no statistical difference in these parameters on seventh day, and the remaining parameters were similar across subgroups. Collagen content was also similar across subgroups. CONCLuSION: In the presence of abdominal sepsis, the administration of bromopride was associated with decreased tensile strength and hydroxyproline concentration in left colonic anastomoses in rats three days after surgery. Key words: Anastomosis, Surgical. Colon. Sepsis. Grastrointestinal Motility. Tissue Adhesions. Rats. RESuMO OBJETIVO:Avaliar os efeitos da bromoprida sobre a cicatrização de anastomoses de cólon esquerdo de ratos na presença de sepse abdominal. MéTOdOS: Quarenta ratos distribuídos em grupos contendo 20 animais para administração de bromoprida ou salina. Cada grupo foi dividido em subgrupos contendo dez animais, para eutanásia no terceiro ou no sétimo dia de pós-operatório. A indução da sepse foi realizada pelo método de ligadura e punção do ceco. Foi realizada ressecção de um segmento do cólon esquerdo e anastomose término-terminal. À re-laparotomia, foi avaliada a quantidade total de aderências e removido um segmento colônico contendo a anastomose para análise histopatológica, força de ruptura, concentração de hidroxiprolina e histomorfometria do colágeno. RESuLTAdOS: No 3° DPO, as anastomoses dos animais tratados com bromoprida apresentaram menor força de ruptura (p=0,02) e maior redução da concentração de hidroxiprolina (p=0,04) que os animais controle. Não houve diferença estatística quanto a estes parâmetros no 7° DPO. O conteúdo de colágeno foi semelhante entre os subgrupos. CONCLuSãO: Na presença de sepse abdominal, o uso da bromoprida esteve associado à diminuição da força de ruptura e da concentração de hidroxiprolina das anastomoses do cólon esquerdo de ratos no 3° DPO. descritores: Anastomose Cirúrgica. Cólon. Sepse. Motilidade Gastrointestinal. Aderências Teciduais. Ratos. Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

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Section: Discussionmentioning

confidence: 99%

Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

Silva¹,

Carneiro

Oliveira

et al. 2012

Acta Cir. Bras.

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“…Healing can also be evaluated by other methods, such as hydroxyproline dosage, hematoxylin-eosin staining histopathological analysis, and tensile strength measurement 29,30 . Since collagen is critical to tissue strength and integrity and is essential in all stages of healing; its measurement in wound deposition was selected for this study [17][18][19]31,32 .…”

Section: ■ Discussionmentioning

confidence: 99%

Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

et al. 2020

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Purpose To determine by histomorphometric analysis whether CO 2 pneumoperitoneum interferes with collagen deposition in surgical wounds in the aponeurosis of rats. Methods This experiment involved 80 male Wistar rats, randomly allocated into four groups according to pneumoperitoneum period (PRE: 30 min preoperatively; POST: 30 min postoperatively; PP: 30 min pre- and postoperatively; C: control group). CO 2 pneumoperitoneum was insufflated to 5 mmHg of pressure. A laparotomy was performed; 1 cm of the left colon was then resected, and an end-to-end anastomosis was performed to simulate surgical trauma, after which the abdominal wall was closed. On postoperative days 7 or 14, a sample of the abdominal wall was collected, stained with picrosirius red and observed under polarized light in an optical microscope. The amount of collagen was estimated by computerized histomorphometric analysis. Results There were no significant differences in collagen deposition between the control and experimental groups on postoperative days 7 (p=0.720) or 14 (p=0.933). The amount of collagen increased as expected in all groups between postoperative days 7 and 14 (p=0.0003). Conclusion At 5 mmHg, CO 2 pneumoperitoneum does not interfere with collagen deposition in abdominal wall surgical wounds in rats.

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“…Intra-abdominal infection could increase the formation of intra-cavitary adhesions and the cecal ligation and puncture model seems to be one of the most important factors responsible for that 10 . The gastrointestinal tract's bacterial flora seems to play an important role in the formation of adhesions around the anastomosis 11 .…”

Section: Discussionmentioning

confidence: 99%

Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

Silva¹,

Oliveira

Brandão

et al. 2011

Acta Cir. Bras.

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PURPOSE:To evaluate the effects of abdominal sepsis on adhesion formation and colon anastomosis healing in rats. METHODS: Forty rats were distributed in two groups containing 20 rats each for left colon anastomosis in the presence (Group S) or absence (Group N) of induced sepsis by cecal ligation and puncture. Each group was divided into subgroups for euthanasia on the third (N3 and S3) or seventh (N7 or S7) post-operative day. The amount of adhesions was evaluated and a segment of the colon was removed for histopathologic analysis, bursting strength assessment, hydroxyproline and the determination of tissue collagen. RESULTS: The subjects which underwent cecal ligation and puncture presented a higher amount of intra-abdominal adherences in both third (p=0,00) and seventh (p=0,00) post-operatory days. Smaller bursting strengths were found in the S3 subgroup, and greater bursting strengths were found in the S7 subgroup. There was no difference in the variations on the concentrations of hydroxyproline, tissue collagen and histopathology. CONCLUSIONS: The peritoneal infection which was developed by cecal ligation and puncture raised the amount of intra-cavitary adhesions. There was a decrease in the amount of colonic anastomosis on the third post-operatory day with a following raise on the seventh without any effects on other healing parameters. Key words: Anastomosis, Surgical. Colon. Sepsis. Tissue Adhesions. Rats. RESUMO OBJETIVO:Avaliar os efeitos da sepse abdominal sobre a formação de aderências e a cicatrização de anastomoses colônicas em ratos. MÉTODOS: 40 ratos distribuídos em dois grupos contendo 20 animais, para anastomose do cólon esquerdo na presença (grupo S) ou ausência (grupo N) de indução de sepse por ligadura e punção do ceco (CLP). Cada grupo foi dividido em subgrupos para eutanásia no terceiro (N3 e S3) ou sétimo (N7 e S7) dia de pós-operatório (DPO). Foi avaliada a quantidade de aderências e removido um segmento colônico contendo a anastomose para análise histopatológica, força de ruptura, hidroxiprolina e conteúdo de colágeno tecidual. RESULTADOS: Os animais submetidos à CLP apresentaram maior quantidade de aderências intra-abdominais tanto no 3° DPO (p=0,00) quanto no 7° DPO (p=0,00). Tiveram menores valores de força de ruptura no 3° DPO (p=0,00), porém maiores valores no 7°D PO (p=0,00). Não houve diferença na variação da concentração de hidroxiprolina, conteúdo de colágeno e histopatologia. CONCLUSÕES: A infecção peritoneal desencadeada por CLP aumentou a quantidade de aderências intra-cavitárias. Houve diminuição da resistência de anastomoses cólicas no 3° DPO, com posterior aumento no 7° DPO, sem efeito sobre os outros parâmetros da cicatrização. Descritores: Anastomose Cirúrgica. Cólon. Sepse. Aderências Teciduais. Ratos. Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

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Effect of Temporary Abdominal Closure on Colonic Anastomosis and Postoperative Adhesions in Experimental Secondary Peritonitis

Abstract: Relaparotomy and the type of temporary closure have no negative effect on anastomotic healing in rats with peritonitis. Temporary abdominal closure with a Bogota bag caused a significantly high rate of adhesions.

Cited by 7 publications

References 61 publications

Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

Effects of bromopride on the healing of left colonic anastomoses in rats with induced abdominal sepsis

Does CO2 pneumoperitoneum in laparoscopy interfere with collagen deposition in abdominal surgical wounds?

Study on adhesion formation and the healing of colon anastomosis in rats with induced peritoneal sepsis

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