Effect of the Antibiotic Neomycin on the Toxicity of the Glycoside Vicine in Rats

Arbid, Mahmoud S.; Koriem, Khaled M. M.; Asaad, Gihan F.; Megahed, Hoda A.

doi:10.1155/2013/913128

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…The decline in WBCs was also observed by many researchers (Arbid and Marquardt,1986; Yanni and Marquardt,1985; Arbid et al , 2013) after the injection of vicine and convivine in male albino rats. On the other side, the oral administration with pickled olives+ V. faba in favism animals' models, pickled olives + vicine in vicine-treated animals and pickled olives + convicine in convicine-treated animals pushed WBCs to the control value.…”

Section: Discussionsupporting

confidence: 71%

Pickled olives neutralize vicine and convicine glycosides in Vicia faba and protect from favism

Koriem¹,

Arbid²

2020

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Purpose This paper aims to evaluate hematological parameters, blood glutathione (GSH), serum glucose-6-phosphate dehydrogenase (G6-PD) and liver function in favism animals' models after oral intake with a mixture of pickled olives and Vicia faba (V. faba) seeds. Design/methodology/approach Favism is a life-threatening hemolytic crisis. It results from the ingestion of V. faba by the individuals. The hemoglobin (Hb), hematocrit (Hct), red blood cells (RBCs), white blood cells (WBCs), serum glucose, blood GSH, serum G6-PD, serum thiobarbaturic acid reactive substances (TBARS), liver protein and liver function were evaluated after oral administration with a mixture of pickled olives with V. faba seeds in favism animals' models. Findings The favism-, vicine- and convicine-treated animals showed a significant decrease (p < 0.01) in Hb (6.42, 7.23 and 5.96 g/dl), Hct (25.4, 26.4 and 25.1%), RBCs (2.56, 2.45 and 2.60 106 cells/mm3, WBCs (4.35, 4.25 and 4.30 103 cells/mm3), serum glucose (95.8, 97.1 and 96.5 mg/dl), blood GSH (24.7, 26.6 and 23.8 mg/dl), serum G6-PD (15.8, 15.9 and 15.7 U/L), serum aspartate aminotransferase (6.35, 6.59 and 5.97 U/L), alanine aminotransferase (4.49, 4.61 and 4.50 U/L), total protein (6.54, 6.59 and 6.40 g/dl), albumin (3.84, 3.91 and 3.75 g/dl), globulin (2.70, 2.48 and 2.65 g/dl) and liver protein (3.37, 3.10 and 3.42 g/g tissue) but a significant increase (p < 0.01) in serum TBARS (38.7, 38.9 and 39.4 nmol/dl), alkaline phosphatase (275, 271 and 281 U/L) and total bilirubin (0.93, 0.89 and 0.91 mg/dl) compared to Hb (16.3 g/dl), Hct (45.3%), RBCs (5.80 106 cells/mm3), WBCs (9.45 103 cells/mm3), serum glucose (96.5 mg/dl), blood GSH (39.7 mg/dl), serum G6-PD (36.1 U/L), serum TBARS (18.0 nmol/dl), serum aspartate aminotransferase (19.8 U/L), alanine aminotransferase (9.23 U/L), alkaline phosphatase (214 U/L), total bilirubin (0.57 mg/dl), total protein (8.76 g/dl), albumin (4.85 g/dl), globulin (3.91 g/dl) and liver protein (6.28 g/g tissue) in control group. The oral administration with pickled olives + V. faba, pickled olives + vicine and pickled olives + convicine into favism animals' models, vicine-treated animals and convicine-treated animals, respectively pushed all the above-mentioned parameters to near the control levels. Originality/value V. faba contains vicine and convicine glycosides. Vicine and convicine glycosides in V. faba are hydrolyzed by intestinal microflora to aglycones divicine and isouramil, respectively. Divicine and isouramil are highly reactive compounds generating free radicals where divicine and isouramil are the main factors of favism. The ß-glucosidase in pickled olives converts both vicine and convicine glycosides into aglycones divicine and isouramil, respectively, in aerobic condition outside the human body (in inactive forms) and prevent these glycosides to cause favism.

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Section: Discussionsupporting

confidence: 71%

Pickled olives neutralize vicine and convicine glycosides in Vicia faba and protect from favism

Koriem¹,

Arbid²

2020

NFS

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“…The cleavage amplified polymorphism markers methods are used to produce faba beans cultivars with low vicine and convicine content and enhanced nutritional use of faba beans [7]. Antibiotic neomycin counteracts the acts of favism inducing factor ''vicine'' in rats [8]. Furthermore, favism induced oxidative stress and genotoxicity which abolished by anise oil treatment [9].…”

Section: Introductionmentioning

confidence: 99%

Supplementation of α-Tocopherol Attenuates Minerals Disturbance, Oxidative Stress and Apoptosis Occurring in Favism

2016

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The favism is a metabolic disease that characterized with an acute hemolytic anemia where α-tocopherol is a type of tocopherol accumulated inside the human body. The objective of such a study was established to evaluate the effect of α-tocopherol in favism disorders. A total of 75 human cases were divided into 5 groups as follow; group 1 normal cases without any treatment and group 2 normal cases orally administrated α-tocopherol (200 mg/kg) once a day over 30 days period. Group 3 favism patients without any treatment. Groups 4 and 5 favism patients orally administrated 100 and 200 mg α-tocopherol/kg, respectively once a day over 30 days period. The results obtained revealed that oral administration of α-tocopherol into normal cases over 30 days period did not induce any biological change. In favism, hemoglobin, hematocrit, red and white blood cells, serum glucose, glucose-6-phosphate dehydrogenase, total protein, albumin, globulin, aspartate and alanine aminotransferases, blood glutathione, superoxide dismutase, glutathione peroxidase and serum calcium, phosphorous, sodium, potassium and chloride levels were significantly decreased. On the other hand, serum alkaline phosphatase, bilirubin, selenium, zinc, manganese, copper and iron, malondialdehyde levels showed significant increase in favism. Supplementation with α-tocopherol into favism restores all the above mentioned parameters to approach the normal levels. Also, α-tocopherol has anti-apoptotic effect in favism. In conclusion, α-tocopherol attenuates minerals disturbance, oxidative stress and apoptosis occurring in favism.

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“…(2) Paraffin oil group: injected IP with 0.5 ml paraffin oil/rat and this oil was used a vehicle for vitamin E because this oil does not contain vitamin E or any antioxidant in contrast to many other oils such as olive oil, soybean oil, sunflower oil and corn oil which contain vitamin E. (3) Vicine and convicine (VC)-treated group: injected IP with 400 mg/kg V (Arbid et al, 2013) dissolved in 0.5 ml of distilled water and after 1 h the same rats injected IP with 150 mg/kg C (Arbid et al, 1993) dissolved in 0.5 ml of distilled water. (4) Vitamin E (100 mg/kg) þ VC-treated group: injected IP vitamin E (100 mg/kg) dissolved in 0.5-ml paraffin oil, 30 minutes before the injection IP of both V and C, daily once a day into VC-treated group.…”

Section: Experimental Designmentioning

confidence: 99%

“…So, an acute hemolysis caused by Vicia faba ingestion in G6PD deficient individuals (favism) is the best studied natural model of oxidative damage (Arese et al, 2012). The antibiotic neomycin and natural products such as anise oil, a-tocopherol, b -carotene and pickled olives were used to overcome the V and C toxicity (Arbid et al, 2013;Koriem et al, 2016Koriem et al, , 2017Koriem et al, , 2018Koriem and Arbid, 2020). Vicia faba contains V at 10-15 mg/g of dry weight and C at 3-5 mg/g of dry weight (Kowalczyk et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Vitamin E ameliorates disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine and convicine of Vicia faba

Koriem

Arbid

2021

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Purpose This paper aims to design to evaluate the protective effect of vitamin E to ameliorate the disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine (V) and convicine (C) of Vicia faba. Design/methodology/approach Forty male albino rats were divided into five equal groups; control, paraffin oil, V (400 mg/kg) C (150 mg/kg)-treated group, vitamin E (100 mg/kg) + VC-treated group, and vitamin E (200 mg/kg) + VC-treated groups which injected intraperioneally (IP) with 0.5-ml saline, 0.5-ml paraffin oil,V (400 mg/kg) and C (150 mg/kg) of Vicia faba, vitamin E (100 mg/kg) + VC-treated groups, and Vitamin E(200 mg/kg) + VC-treated groups, respectively. Blood and testicular tissue were obtained after one month of the study. The male genital organs were calculated. Testosterone (Ts), luteinizing hormone (LH), follicle stimulating hormone (FSH), dehydroepiandrosterone sulfate (DHEA-SO4), sex hormone binding globulin (SHBG),?-glutamyl transpeptidase (?-GT), glucose-6-phosphate dehydrogenase (G6PD), 3ß-hydroxysteroid dehydrogenase (3ßHSD), lactate dehydrogenase (LDH), spermatozoa concentration, percent of mortality and abnormal sperms were evaluated. Findings The VC-treated group showed significant decrease (p < 0.01) in Ts, DHEA-SO4, G6PD, spermatozoa number and mortality percent, as well as, the male genital organs (testes, epidydemis, seminal vesicle, prostate and vasa deferentia) while significant increase (p < 0.01) was found in LH, FSH, SHBG, LDH, ?-GT, sperms monoclonal Ki-67, and abnormal spermatocytes levels compared with control group. Vitamin E co-injection with VC-treated group returned all these parameters to the normal values. The higher dose of vitamin E (200 mg/kg) was more effect than the lower dose (100 mg/kg). Originality/value Vicia faba contains V and C glycosides. The V and C glycosides in Vicia faba are hydrolyzed by intestinal microflora to aglycones divicine and isouramil, respectively. Divicine and isouramil are highly reactive compounds generating free radicals where divicine and isouramil are the main factors of favism. The V and C glycosides induced disturbances in testosterone pathway and sperm quality of male rats and vitamin E ameliorates these disturbances.

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Effect of the Antibiotic Neomycin on the Toxicity of the Glycoside Vicine in Rats

Cited by 10 publications

References 29 publications

Pickled olives neutralize vicine and convicine glycosides in Vicia faba and protect from favism

Pickled olives neutralize vicine and convicine glycosides in Vicia faba and protect from favism

Supplementation of α-Tocopherol Attenuates Minerals Disturbance, Oxidative Stress and Apoptosis Occurring in Favism

Vitamin E ameliorates disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine and convicine of Vicia faba

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