Effect of the freeze-drying process on the phenotypic diversity of Pseudomonas putida strains isolated from the interior of healthy roots of Sida hermaphrodita: Phenotype microarrays (PMs)

Jałowiecki, Łukasz; Krzymińska, Izabela; Górska, M.; Płaza, Grażyna; Ratman-Kłosińska, Izabela

doi:10.1016/j.cryobiol.2020.07.004

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…We can therefore argue that the surviving 10% of the population was somehow selected by the storage. It was also reported that freeze drying influenced the antibiotic resistance of the preserved strain [34]. Therefore, the selection of mutants in the lyovials of A. coloradensis could arise from the long-term storage under suboptimal conditions.…”

Section: Discussionmentioning

confidence: 99%

New Avoparcin-like Molecules from the Avoparcin Producer Amycolatopsis coloradensis ATCC 53629

ramoni¹,

Capozzoli²,

Bava³

et al. 2022

Fermentation

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Amycolatopsis coloradensis ATCC 53629 is the producer of the glycopeptide antibiotic avoparcin. While setting up the production of the avoparcin complex, in view of its use as analytical standard, we uncovered the production of a to-date not described ristosamynil-avoparcin. Ristosamynil-avoparcin is produced together with α- and β-avoparcin (overall indicated as the avoparcin complex). Selection of one high producer morphological variant within the A. coloradensis population, together with the use of a new fermentation medium, allowed to increase productivity of the avoparcin complex up to 9 g/L in flask fermentations. The selected high producer displayed a non-spore forming phenotype. All the selected phenotypes, as well as the original unselected population, displayed invariably the ability to produce a complex rich in ristosamynil-avoparcin. This suggested that the original strain deposited was not conforming to the description or that long term storage of the lyovials has selected mutants from the original population.

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Section: Discussionmentioning

confidence: 99%

New Avoparcin-like Molecules from the Avoparcin Producer Amycolatopsis coloradensis ATCC 53629

ramoni¹,

Capozzoli²,

Bava³

et al. 2022

Fermentation

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“…Subsequently, the previously prepared solutions according to the BIOLOG™ method (Biolog Inc., Hayward, USA) were added to the suspension so that a transmittance of 85% was obtained. The detailed methodology of the prepared solutions is described, among others, in the paper by Jałowiecki et al (2020). Subsequently, the solutions together with the suspension of microorganisms were applied to PM plates using a multichannel pipette, 100 µl per well.…”

Section: Phenotypic Characterization By Biolog Phenotype Microarrays ...mentioning

confidence: 99%

Searching of Phenol-Degrading Bacteria in Raw Wastewater from Underground Coal Gasification Process as Suitable Candidates in Bioaugmenation Approach

Jałowiecki,

Borgulat,

Strugała-Wilczek

et al. 2024

J. Ecol. Eng.

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The aim of the conducted study was to isolate, identify and characterize suitable bacterial strains from UCG wastewater as potential candidates for the bioaugmentation approach. For this purpose, the straightforward cultivation procedure and unique biochemical selection were employed to gain insights into the specific properties of bacteria. From the 100 strains isolated from UCG wastewater, three (Paenibacillus pasadensis SAFN-007, Peanibacillus humicus Au34, and Staphylococcus warneri DK131) demonstrated the capacity to degrade phenol and specific biochemical properties. Phenol degradation reached more than 90% for the above-mentioned strains, while the average phenol removal rate for other selected strains was 82.9%, ranging from 66.1% to 90%. The bacterial strains belong to multi-enzyme producers and constitute a possible source of potential technologically important enzymes. Phenotypic microarray plates were used to characterise the metabolic properties of the strains. It was found that 74%, 67.4% and 94.2% of the carbon metabolites tested were utilised by Paenibacillus pasadensis SAFN-007, Peanibacillus humicus Au34 and Staphylococcus warneri DK131, respectively. Among C sources, the strains have the capability to metabolize some substrates appearing in phenol pathways, such as: N-acetyl-D-glucosamine, succinic acid, α-hydroxy-glutaric acid-γ-lactone, bromosuccinic acid, mono-methyl succinate, methyl-pyruvate, p-hydroxy-phenyl acetic acid, m-hydroxyphenylacetic acid, L-galactonic acid-γ-lactone, D-galactonic acid-γlactone, phenylethylamine. Bacteria show different levels of tolerance to pH and osmolality, and they can thrive in different habitats. Another characteristic of these strains is their high resistance to many antibiotics (multi-resistant bacteria). These properties allow the use of the isolated bacterial strains as good candidates for bioremediation of phenol-contaminated environments. The wastewater from the underground coal gasification process is an example of a good extreme environment for the isolation of unique bacteria with specific metabolic properties.

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“…Lyophilization, also known as freeze-drying, is widely used in various fields, such as pharmaceutical, cosmeceutical, nutraceutical, chemical, and food industries [ 1 , 2 ]. It can be used for, e.g., the preservation of sensitive materials such as microorganisms, nanoparticulate systems, nucleic acids, and proteins, as well as to improve drug stability [ 3 , 4 ]; to stabilize vaccine formulations [ 5 ]; to preserve microorganisms [ 6 ]; to pre-process for the storage of serum and plasma [ 7 ]; to dry polymer nanoparticles [ 8 ], plant extracts [ 1 ] or crude plant material [ 9 ]; and to preserve food and increase the shelf life of agricultural products [ 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Lyophilization can also lead to undesirable side effects, e.g., long and variable reconstitution times of lyophilized products containing highly concentrated proteins [ 25 ]. Other disadvantages that were reported for lyophilization are the potential loss of volatile compounds that are prone to sublimation [ 10 ], decreased metabolic activities of certain bacterial strains and their antibiotic/chemical resistance [ 6 ], and various stresses that can lead to chemical and physical instabilities of nanoparticles, vaccines, genes, and microorganisms (e.g., physical alteration caused by the removal of the molecule hydration shell or stresses related to the internal environment of bacteria, such as osmolarity change or ice formation) [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Untargeted Plant Metabolomics: Evaluation of Lyophilization as a Sample Preparation Technique

Maisl,

Doppler,

Seidl

et al. 2023

Metabolites

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Lyophilization is a common method used for stabilizing biological samples prior to storage or to concentrate extracts. However, it is possible that this process may alter the metabolic composition or lead to the loss of metabolites. In this study, the performance of lyophilization is investigated in the example of wheat roots. To this end, native and 13C-labelled, fresh or already lyophilized root samples, and (diluted) extracts with dilution factors up to 32 and authentic reference standards were investigated. All samples were analyzed using RP-LC-HRMS. Results show that using lyophilization for the stabilization of plant material altered the metabolic sample composition. Overall, 7% of all wheat metabolites detected in non-lyophilized samples were not detected in dried samples anymore, and up to 43% of the remaining metabolites exhibited significantly increased or decreased abundances. With respect to extract concentration, less than 5% of the expected metabolites were completely lost by lyophilization and the recovery rates of the remaining metabolites were slightly reduced with increasing concentration factors to an average of 85% at an enrichment factor of 32. Compound annotation did not indicate specific classes of wheat metabolites to be affected.

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Effect of the freeze-drying process on the phenotypic diversity of Pseudomonas putida strains isolated from the interior of healthy roots of Sida hermaphrodita: Phenotype microarrays (PMs)

Cited by 7 publications

References 34 publications

New Avoparcin-like Molecules from the Avoparcin Producer Amycolatopsis coloradensis ATCC 53629

New Avoparcin-like Molecules from the Avoparcin Producer Amycolatopsis coloradensis ATCC 53629

Searching of Phenol-Degrading Bacteria in Raw Wastewater from Underground Coal Gasification Process as Suitable Candidates in Bioaugmenation Approach

Untargeted Plant Metabolomics: Evaluation of Lyophilization as a Sample Preparation Technique

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