Effect of the higher-order structure of tRNAs on the stability of hybrids with oligodeoxyribonucleotides: separation of tRNA by an efficient solution hybridization

Kumazawa, Yoshinori; Yokogawa, Takashi; Tsurui, Hiromichi; Miura, Kiyonori; Watanabe, Kimitsuna

doi:10.1093/nar/20.9.2223

Cited by 38 publications

(31 citation statements)

References 26 publications

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“…The large difference in the yields of these two tRNAs is probably due mainly to the different amounts of each tRNA contained in the cells and slightly to the hybridizing efficiency of the DNA probes [19].…”

Section: Resultsmentioning

confidence: 99%

A new method for identifying the amino acid attached to a particular RNA in the cell

1996

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To investigate the function of tRNAs or any other aminoacylable RNAs in vivo, it is important to be able to estimate the amounts and species of aminoacylated RNAs in living cells. We have developed a method of analyzing amino acids attached to particular tRNAs obtained from cells. After the ester bond between the amino acid and the 3'-adenosine moiety of a specific aminoacyl-tRNA is stabilized by acetylation of the amino acid with [14C]acetic anhydride, the aminoacyl-tRNA can be fished out with a solid-phase-attached DNA probe. The t4C-labeled acetylamino acid is then released from the thus purified acetyl-aminoacyl-tRNAs by alkaline treatment and detected by TLC analysis.

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Section: Resultsmentioning

confidence: 99%

A new method for identifying the amino acid attached to a particular RNA in the cell

1996

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“…3 which was probed with the same amount of radioactivity in probes of the same specific activity). Oligonucleotide V647 failed to hybridize to either strong-stop DNA (data not shown), most likely owing to the large differences reported for hybridization efficiencies to different regions of the tRNAs (Kumazawa et al, 1992). From these results, we conclude that the strong-stop DNA of RTBV consists of 595 nt of DNA with about 70 ribonucleotides covalently linked to it.…”

Section: Resultsmentioning

confidence: 84%

A strong-stop DNA in rice plants infected with rice tungro bacilliform virus

Bao

Hull²

1993

Journal of General Virology

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A virus-specific small nucleic acid (strong-stop DNA) was identified in rice plants infected with rice tungro bacilliform virus, but not in the virus particles. This nucleic acid was shown to consist of about 595 deoxyribonucleotides with about 70 ribonucleotides covalently linked at the 5' end. Hybridization with sequence-specific oligonucleotides showed that the ribonucleotides were from the plant cytoplasmic tRNA~ ~t sequence. PCR analysis detected hairpin structures at the 3' end of the DNA.

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“…Filter and solution hybridization assays were used for the characterization of yeast cytosolic tRNA Phe and bovine mitochondrial tRNA Ser [17][18][19]. Dong et al used two-dimensional polyacrylamide gel electrophoresis to fractionate tRNA from Escherichia coli, and isolated components were identified by hybridization to tRNA-specific oligonucleotide probes [20].…”

Section: Introductionmentioning

confidence: 99%

Global identification of transfer RNAs by liquid chromatography–mass spectrometry (LC–MS)

Wetzel

Limbach

2012

Journal of Proteomics

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Effect of the higher-order structure of tRNAs on the stability of hybrids with oligodeoxyribonucleotides: separation of tRNA by an efficient solution hybridization

Abstract: ABSTRACT

Cited by 38 publications

References 26 publications

A new method for identifying the amino acid attached to a particular RNA in the cell

A new method for identifying the amino acid attached to a particular RNA in the cell

A strong-stop DNA in rice plants infected with rice tungro bacilliform virus

Global identification of transfer RNAs by liquid chromatography–mass spectrometry (LC–MS)

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