Effect of the Method for Immobilizing Receptor Layer on the Analytical Characteristics of a Label-Free Electrochemical Immunosensor for the Determination of Measles Antibodies

Свалова, Т. С.; Малышева, Н. Н.; Бубекова, А. К.; Saigushkina, Anna A.; Medvedeva, M. V.; Козицина, А. Н.

doi:10.1134/s106193482002015x

Cited by 7 publications

(2 citation statements)

References 38 publications

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“…Electrochemical labels, electroactive mediators (ferrocene and ferricyanide), and enzymatic labels are used to record the electrochemical signal. Such immunosensors have been widely used to detect various types of viruses, including hepatitis B [157][158][159][160][161][162][163] and C [164][165][166][167], influenza viruses [168][169][170][171][172], Dengue [173][174][175], Zika [176], Japanese encephalitis [177,178], measles [179], Newcastle disease virus [180], and enterovirus [181]. These types of sensors are considered to be a complementary approach to traditional quantitative RT-PCR.…”

Section: Electrochemicalmentioning

confidence: 99%

Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example

Zhdanov,

Ivin,

Shishparenok

et al. 2023

BIOMED KHIM

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Traditional antiviral vaccines are currently created by inactivating the virus chemically, most often using formaldehyde or β-propiolactone. These approaches are not optimal since they negatively affect the safety of the antigenic determinants of the inactivated particles and require additional purification stages. The most promising platforms for creating vaccines are based on pseudoviruses, i.e., viruses that have completely preserved the outer shell (capsid), while losing the ability to reproduce owing to the destruction of the genome. The irradiation of viruses with electron beam is the optimal way to create pseudoviral particles. In this review, with the example of the poliovirus, the main algorithms that can be applied to characterize pseudoviral particles functionally and structurally in the process of creating a vaccine preparation are presented. These algorithms are, namely, the analysis of the degree of genome destruction and coimmunogenicity. The structure of the poliovirus and methods of its inactivation are considered. Methods for assessing residual infectivity and immunogenicity are proposed for the functional characterization of pseudoviruses. Genome integrity analysis approaches, atomic force and electron microscopy, surface plasmon resonance, and bioelectrochemical methods are crucial to structural characterization of the pseudovirus particles.

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Section: Electrochemicalmentioning

confidence: 99%

Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example

Zhdanov,

Ivin,

Shishparenok

et al. 2023

BIOMED KHIM

View full text Add to dashboard Cite

show abstract

“…[13,14] Several works have been published on the POC detection of measles-specific IgM in serum buffer. [15][16][17][18] This approach, however, is limited to the diagnosis of the pathology following the immune response of the human host. Therefore, it is hardly applicable in cases where the patient's immune system is compromised, or antibody production is delayed compared to overt disease.…”

Section: Introductionmentioning

confidence: 99%

Surface‐Acoustic‐Wave (SAW) Induced Mixing Enhances the Detection of Viruses: Application to Measles Sensing in Whole Human Saliva with a SAW Lab‐On‐a‐Chip

Agostini¹,

Lunardelli

Gagliardi

et al. 2022

Adv Funct Materials

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Measles is one of the most infectious airborne viruses worldwide. With a basic reproduction rate between 12-18, this virus is six times more infectious than the SARS-CoV-2 Alpha variant and similar to the SARS-CoV-2 Omicron variant. Even though a cheap and effective vaccine is available, measles is still common in developing countries. To date, sporadic outbreaks are also reported in developed countries, primarily due to non-vaccinated people. This work presents a point-of-care (POC) biosensing device capable of detecting measles virions (MV) in human saliva. The device is a surface-acoustic-wave (SAW) based lab-on-a-chip (LOC), smaller than a €1-cent coin, in which SAWs are used both for sensing and fluid recirculation. The biosensing detection performance of this system is tested and device sensitivity and selectivity are assessed. The SAW-LOC with MV loaded in healthy, whole human saliva is finally validated. The experimental results also highlight a crucial aspect of the biosensing process: the interactions between probing and target species during incubation with or without fluid mixing. The presented findings are promising for realizing a POC platform for measles diagnosis and may serve as a guideline for designing new microfluidics-based biosensing systems.

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Fundamentals for Virus and Antigen Detection in Immunotechnologies

Castro

Almeida

Faria

et al. 2022

Advances in Bioelectrochemistry Volume 3

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Effect of the Method for Immobilizing Receptor Layer on the Analytical Characteristics of a Label-Free Electrochemical Immunosensor for the Determination of Measles Antibodies

Cited by 7 publications

References 38 publications

Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example

Perspectives for the creation of a new type of vaccine preparations based on pseudovirus particles using polio vaccine as an example

Surface‐Acoustic‐Wave (SAW) Induced Mixing Enhances the Detection of Viruses: Application to Measles Sensing in Whole Human Saliva with a SAW Lab‐On‐a‐Chip

Fundamentals for Virus and Antigen Detection in Immunotechnologies

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