Effect of the Modified Methacrylate-Based Root Canal Sealer in Single-Cone Technique

Abstract

This study aimed to modify EndoREZ with 2.5% dimethylaminododecyl methacrylate (DMADDM) and 1% magnetic nanoparticles (MNP) to study its sealing property, penetration and long-term antibacterial and therapeutic effect in the single-cone technique (SCT) compared with EndoREZ and iRoot SP. Thirty single-root human maxillary premolars were assigned into three groups and obturated with three different root canal sealers by SCT. Every specimen was then scanned using micro-CT to analyze void fraction, and void volumes and confocal laser scanning microscope (CLSM) was used to study the dentin penetration. The long-term antimicrobial effects were tested in vitro before and after aging 1 and 4 weeks by the single-strain Enterococcus faecalis biofilm model. In addition, the beagle canine model of apical periodontitis (AP) was utilized to judge and compare the therapeutic effect of three sealers in SCT. The void fraction and void volumes of the modified root canal sealer were not significantly different from iRoot SP (p > 0.05) but were lower than EndoREZ (p < 0.05). The modified root canal sealant displayed a greater penetration, long-term antibacterial property, and treatment effect than the other groups (p < 0.05). This indicated that after being modified with DMADDM and MNP, it showed better performance in SCT.

Application of Nanomaterials in Endodontics

“…Other metal or metal oxide NPs are also of interest as materials that could be added into existing root canal sealers. One recent study incorporated ferrimagnetic magnetite (Fe 3 O 4 ) NPs (50 to 100 nm) and a quaternary ammonium salt (QAS) into EndoREZ sealer [ 132 ]. By applying an external magnetic field, dentinal tubule penetration was enhanced.…”

Nanoarchitectonics-Based Materials as a Promising Strategy in the Treatment of Endodontic Infections

“…The addition of dimethylaminododecyl methacrylate (DMADDM) into different dental materials can improve antibacterial effects against pathogens and biofilms. Another group has aimed to modify EndoREZ with DMADDM and magnetic nanoparticles (MNPs) [ 195 ]. The modification can lead to good sealing performance, penetration, and long-term antibacterial properties.…”

“…However, the AgNPs used did not improve the bacterial leakage resistance of the AH plus sealer after 3 months of observation [ 193 ]. Nanoparticles of ZnO were also added to the original sealer and this modification inhibited biofilm formation within the sealer– dentin interface, reduced cytotoxicity, and improved physicochemical properties [ 195 ]. It was concluded that the addition of ZnO NPs into a calcium hydroxide-based sealer shows better antibiofilm efficacy than CSNPs against E. faecalis .…”