Effect of the Steaming Process on Quality of Postharvest &lt;i&gt;Cistanche deserticola&lt;/i&gt; for Medicinal Use during Sun Drying

Peng, Fang; Xu, Ru; Wang, Xia; Xu, Chunping; Liu, Tong-Ning; Chen, Jun

doi:10.1248/bpb.b16-00250

Cited by 10 publications

(7 citation statements)

References 15 publications

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“…Iridoids possess anti-inflammatory activities [ 15 , 16 ]. It has also been revealed by earlier studies that some chemical components showed variations during the processing [ 17 – 20 ]. Based on these reports, it can be assumed that post-processing, the variations in chemical composition lead to various pharmacological effects, which need to be further explored.…”

Section: Introductionmentioning

confidence: 86%

Chemical profiles and metabolite study of raw and processed Cistanche deserticola in rats by UPLC-Q-TOF-MSE

Ryenchindorj²,

Liu

et al. 2021

Chin Med

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Background Chinese materia medica processing is a distinguished and unique pharmaceutical technique in Traditional Chinese Medicine (TCM) used for reducing side effects, and increasing or even changing therapeutic efficacy of the raw herbs.Changes in the essential components induced by an optimized processing procedure are primarily responsible for the increased efficacy of medicinal plants.The kidney-yang invigorating effect of rice wine-steamed Cistancha deserticola (C. deserticola) was stronger than raw C. deserticola (CD). Methods A comparison analysis was carried out using the UPLC-Q-TOF-MSE with the UNIFI informatics platform to determine the influence of processing. In vitro studies were performed for the characterization of constituents as well as metabolites in vivo. The chemical components were determined in CD and its processed products. The multivariate statistical analyses were conducted to evaluate variations between them while OPLS-DA was used for pairwise comparison. Results The results of this study revealed considerable variations in phenylethanoid glycosides (PhGs) and iridoids after processing. A total of 97 compounds were detected in the extracts of CD and its processed product. PhGs having 4'-O-caffeoyl group in the 8-O-β-d-glucopyranosyl part, like acteoside, cistanoside C, campneoside II, osmanthuside decreased after being processed, while PhGs with 6'-O-caffeoyl group in the 8-O-β-d-glucopyranosyl part, such as isoacetoside, isocistanoside C, isocampneoside I, isomartynoside increased, especially in the CD-NP group. The intensity of echinacoside and cistanoside B whose structure possess 6'-O-β-d-glucopyranosyl moiety also increased. In in vivo study, 10 prototype components and 44 metabolites were detected in rat plasma, feces, and urine. The obtained results revealed that processing leads to the considerable variation in the chemical constituents of CD and affected the disposition of the compounds in vivo, and phase II metabolic processes are the key cascades of each compound and most of the metabolites are associated with echinacoside or acteoside. Conclusions This is the first global comparison research of raw and processed CD. These findings add to our understanding of the impact of CD processing and give important data for future efficacy investigations.

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Section: Introductionmentioning

confidence: 86%

Chemical profiles and metabolite study of raw and processed Cistanche deserticola in rats by UPLC-Q-TOF-MSE

Ryenchindorj²,

Liu

et al. 2021

Chin Med

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“…(9). Previous studies have shown that steaming can promote the accumulation of active ingredients in Cistanche deserticola, such as phenylethanoid glycosides, soluble sugars, and polysaccharides, accompanied by blackening of appearance color (9)(10)(11). However, most of the previous studies focused on certain specific compounds, very rare research are about the changes in all the chemical compounds and the metabolite conversion mechanism during processing.…”

Section: Introductionmentioning

confidence: 99%

“…Usually, the harvested Cistanche deserticola rhizome was steamed in a steaming boiler at 93°C for 30 min and then dried at 60°C until the moisture content of 10% on a wet basis (w.b.) ( 9 ). Previous studies have shown that steaming can promote the accumulation of active ingredients in Cistanche deserticola , such as phenylethanoid glycosides, soluble sugars, and polysaccharides, accompanied by blackening of appearance color ( 9 – 11 ).…”

Section: Introductionmentioning

confidence: 99%

Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

Zhang

et al. 2021

Front. Nutr.

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Cistanche deserticola is one of the most precious plants, traditionally as Chinese medicine, and has recently been used in pharmaceutical and healthy food industries. Steaming and drying are two important steps in the processing of Cistanche deserticola. Unfortunately, a comprehensive understanding of the chemical composition changes of Cistanche deserticola during thermal processing is limited. In this study, ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based widely targeted metabolomics analysis was used to investigate the transformation mechanism of Cistanche deserticola active compounds during steaming and drying processes. A total of 776 metabolites were identified in Cistanche deserticola during thermal processing, among which, 77 metabolites were differentially regulated (p < 0.05) wherein 39 were upregulated (UR) and 38 were downregulated (DR). Forty-seven (17 UR, 30 DR) and 30 (22 UR, 8 DR) differential metabolites were identified during steaming and drying, respectively. The most variation of the chemicals was observed during the process of steaming. Metabolic pathway analysis indicated that phenylpropanoid, flavonoid biosynthesis, and alanine metabolism were observed during steaming, while glycine, serine, and threonine metabolism, thiamine metabolism, and unsaturated fatty acid biosynthesis were observed during drying. The possible mechanisms of the chemical alterations during thermal processing were also provided by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Furthermore, the blackening of the appearance of Cistanche deserticola mainly occurred in the steaming stage rather than the drying stage, which is associated with the metabolism of the amino acids. All results indicated that the formation of active compounds during the processing of Cistanche deserticola mainly occurred in the steaming stage.

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“…Iridoids possess anti-inflammatory activities [15][16]. It has also been revealed by earlier studies that some chemical components showed variations during the processing [17][18][19][20]. Based on underlined reports, it has also been considered that post-processing, the variations in chemical composition led to various pharmacological effects, which needs to be further explored.…”

Section: Introductionmentioning

confidence: 99%

Chemical Profiles and Metabolite Study of Raw and Processed Cistanche Deserticola in rats by UPLC-Q-TOF-MSE

Ryenchindorj²,

Liu

et al. 2021

Preprint

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Background: Chinese materia medica processing is a distinguished and unique pharmaceutical technique in traditional Chinese Medicien (TCM), which has played an important role in reducing side effects, increasing medical potencies, altering the properties and even changing the curative effects of raw herbs.The efficacy improvement in medicinal plants is mainly caused by changes in the key substances through an optimized processing procedure.The effect of invigorating the kidney-yang for rice wine-steamed Cistancha deserticola was more strengthened than raw C. deserticola (CD). Methods: To evaluate the effect of processing, a comparative analysis was conducted by utilizing the UPLC-Q-TOF-MSE with the UNIFI informatics platform. In vitro studies were performed for the characterization of constituents as well as metabolites in vivo , and chemical components were determined in CD and its processed products. The multivariate statistical analyses were conducted to evaluate variations between them. OPLS-DA was used for pairwise comparison which revealed their marked differences. Results: In this study, the obtained results revealed considerable variations in phenylethanoid glycosides (PhGs) and iridoids after processing. The detection of 97 compounds was carried out in the extracts of CD and its processed product. In an in-vivo study, 10 prototype components and 44 metabolites were evaluated in rat plasma, feces, and urine. The obtained results revealed that processing leads to the considerable variation in the chemical constituents of CD and affects the disposition of the compounds in-vivo, and phase II metabolic processes were the key cascades of each compound and most of the metabolites were associated with echinacoside or acteoside. Conclusions: According to our literature search, the existing study reveals a comparative study of raw and processed CD for the first time. The obtained data help us to understand the impact of CD processing for further studies.

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Effect of the Steaming Process on Quality of Postharvest <i>Cistanche deserticola</i> for Medicinal Use during Sun Drying

Cited by 10 publications

References 15 publications

Chemical profiles and metabolite study of raw and processed Cistanche deserticola in rats by UPLC-Q-TOF-MSE

Chemical profiles and metabolite study of raw and processed Cistanche deserticola in rats by UPLC-Q-TOF-MSE

Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

Chemical Profiles and Metabolite Study of Raw and Processed Cistanche Deserticola in rats by UPLC-Q-TOF-MSE

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