Effect of the transdermal low-level laser therapy on endothelial function

Abstract

The effect of low-level laser therapy (LLLT) on the cardiovascular system is not fully established. Since the endothelium is an important endocrine element, establishing the mechanisms of LLLT action is an important issue.The aim of the study was to evaluate the effect of transdermal LLLT on endothelial function.In this study, healthy volunteers (n = 40, age = 20–40 years) were enrolled. N = 30 (14 female, 16 male, mean age 30 ± 5 years) constituted the laser-irradiated group (LG). The remaining 10 subjects (6 women, 4 men, mean age 28 ± 5 years) constituted the control group (CG). Participants were subjected to LLLT once a day for three consecutive days. Blood for biochemical assessments was drawn before the first irradiation and 24 h after the last session. In the LG, transdermal illumination of radial artery was conducted (a semiconductor laser λ = 808 nm, irradiation 50 mW, energy density 1.6 W/cm2 and a dose 20 J/day, a total dose of 60 J). Biochemical parameters (reflecting angiogenesis: vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), angiostatin; antioxidative status: glutathione (GSH) and the nitric oxide metabolic pathway: symmetric dimethylarginine (SDMA), asymmetric dimethylarginine (ADMA) and l-arginine) were assessed. In the LG, a significant increase in GSH levels and considerable decrease in angiostatin concentration following the LLLT were observed. No significant differences in levels of the VEGF, FGF, SDMA, ADMA were observed.LLLT modifies vascular endothelial function by increasing its antioxidant and angiogenic potential. We found no significant differences in levels of the nitric oxide pathway metabolites within 24 h following the LLLT irradiation.

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“…A low-power laser at 632.5 nm can enhance eNOS expression in endothelial cells, thereby promoting endothelial cell proliferation and migration, which plays an important role in angiogenesis. 10,15,16 TPKR/PLC-gamma/PKC Pathway The activation of PLC can catalyze phospholipids, thereby increasing the concentration of DAG, IP3. IP3 can increase calcium from the endoplasmic reticulum that activates PKC.…”

Phototherapy as a Rational Antioxidant Treatment Modality in COVID-19 Management; New Concept and Strategic Approach: Critical Review

“…Transdermal PBM (t-PBM) is another therapy that has beneficial effects on the endothelium and blood flow [106]. Studies have shown that PBMT improves the immune system [107][108][109]. A study conducted by Szmcyszyn et al (2016) to evaluate the effects of t-PBM therapy on endothelial function [107].…”

“…Studies have shown that PBMT improves the immune system [107][108][109]. A study conducted by Szmcyszyn et al (2016) to evaluate the effects of t-PBM therapy on endothelial function [107]. Forty healthy young individuals (20-40 years old) were recruited and divided into two groups: laser group (n = 30) and control group (n = 10).…”

Effect of low-level laser therapy (LLLT) and light-emitting diodes (LEDT) applied during combined training on performance and post-exercise recovery: protocol for a randomized placebo-controlled trial

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