Effect of the tryptone concentration on the calcium carbonate biomineralization mediated by Bacillus cereus

Pineda, Gerardo Andrés Caicedo; Prada-Fonseca, María Consuelo; Casas-Botero, Ana Elisa; Tejada, Hader Vladimir Martínez

doi:10.15446/dyna.v85n205.60637

Cited by 8 publications

(4 citation statements)

References 26 publications

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“…35,36 As suggested by Ghosh et al, 37 such elements could donate stabilization for biosynthesized precipitates. Generally, the presence of Ca with the highest intensity in almost all precipitated samples suggested being in higher purity structure as reported by Caicedo-Pineda et al 38 The mineral phase of precipitated crystals was identified using XRD analysis (Fig. 2).…”

Section: Crystal Identification and Characterizationsupporting

confidence: 65%

Heavy metals bioremediation and water softening using ureolytic strains Metschnikowia pulcherrima and Raoultella planticola

Eltarahony

Kamal

Zaki

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND: The recalcitrant nature of lead (Pb) and mercury (Hg) to degradation represents a real risk to living creatures and their surrounding ecosystems. Therefore, this study employed ureolytic fungal strain Metschnikowia pulcherrima (29A) and bacterial strain Raoultella planticola (VIP) in their removal using the promising CaCO 3 bioprecipitation technique, for the first time.RESULTS: Out of 50 isolates, strains 29A and VIP were selected based on their highest ureolytic activity, followed by minimum inhibitory concentration assay using 350 ppm of Pb 2+ and Hg 2+ . Throughout 7 days of incubation at 30°C and 150 rpm with 10 8 CFU mL −1 inoculum, the maximum urease activity was 884 and 639 U mL −1 for 29A and VIP at 24 and 30 h of incubation, respectively. Complete removal of Pb 2+ was achieved at 42 h (29A) and 90 h (VIP), while Hg 2+ was totally removed at 60 h (29A) and 102 h (VIP). Remarkable removal of Ca 2+ (>95%) was achieved by the end of the experiments, which would address the hardness problem in water treatment processes. Further, energy-dispersive X-ray analysis (EDX), scanning electron microscopy (SEM) and X-ray diffraction (XRD) were used to characterize the remediated precipitates. EDX profiles showed characteristic peaks of C, O and Ca besides Pb and Hg. SEM illustrated the presence of microbial imprints and calcinated cells in the remediated bioliths. However, XRD confirmed transformation of soluble metals to insoluble forms entrapped in calcite or vaterite lattice.CONCLUSIONS: Such a bioremediation approach ensures the detoxification and sequestration of heavy metals in stable and durable matrices, hindering their leaching from carbonate complex trap to the environment.

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Section: Crystal Identification and Characterizationsupporting

confidence: 65%

Heavy metals bioremediation and water softening using ureolytic strains Metschnikowia pulcherrima and Raoultella planticola

Eltarahony

Kamal

Zaki

et al. 2021

J of Chemical Tech & Biotech

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show abstract

“…As referred by Ghosh et al, 2019, such elements could donate stabilization for biosynthesized precipitates. Generally, the presence of Ca with the highest intensity in almost of precipitated samples proposed being in higher purity structure as denoted by Caicedo-Pineda et al, (2018).…”

Section: Crystals Identification and Characterizationmentioning

confidence: 99%

Heavy Metals Bioremediation and Water Softening Using Ureolytic Metschnikowia Pulcherrima and Raoultella Planticola Strains

Eltarahony¹,

Kamal²,

Zaki³

et al. 2021

Preprint

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This study employed the ureolytic fungal Metschnikowia pulcherrima (29A) and bacterial Raoultella planticola (VIP) strains in Pb 2+ and Hg 2+ removal using the promising CaCO 3 bio precipitation technique. Out of fifty isolates, strains 29A and VIP were selected based on their highest ureolytic activity followed by MIC assay using 350 ppm of Pb 2+ and Hg 2+ . The maximum urease activity recorded 884 and 639 U/mL for 29A and VIP strains at 24 and 30h of incubation, respectively. Complete removal of Pb 2+ was achieved at 42h and 90h for 29A, VIP correspondingly, while Hg 2+ was totally removed at 60h and 102h for 29A, VIP respectively. Remarkable removal of Ca 2+ (>95%) was achieved by the end of the experiments, which would address the hardness problem in the water treatment process. Further, EDX, SEM and, XRD were used to characterize the remediated precipitates. EDX profiles showed characteristic peaks of C, O and, Ca 2+ besides Pb 2+ and Hg 2+ . SEM illustrated the presence of microbial imprints and calcinated cells in the remediated bioliths. However, XRD confirmed the transformation of soluble metals to insoluble forms entrapped in calcite or vaterite lattice. Such a bioremediation approach ensures the detoxification and sequestration of heavy metals in a stable and durable matrix; obstructing their leach from carbonate complex trap to the environment.

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“…It was hypothesized that the CaCO 3 was produced during the metabolic activity of the bacteria under the influence of calcium acetate. Also, it was found that with the increase and decrease in tryptone concentration, particles with high vaterite phase and calcite phase were synthesized, respectively (Caicedo-Pineda et al 2018).…”

Section: Microbial Synthesismentioning

confidence: 99%

Calcium carbonate nano- and microparticles: synthesis methods and biological applications

et al. 2021

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Calcium carbonate micro-and nanoparticles are considered as chemically inert materials. Therefore, they are widely considered in the field of biosensing, drug delivery, and as filler material in plastic, paper, paint, sealant, and adhesive industries. The unusual properties of calcium carbonate-based nanomaterials, such as biocompatibility, high surface-to-volume ratio, robust nature, easy synthesis, and surface functionalization, and ability to exist in a variety of morphologies and polymorphs, make them an ideal candidate for both industrial and biomedical applications. Significant research efforts have been devoted for developing novel synthesis methods of calcium carbonate particles in micrometer and nanometer dimensions. This review highlights different approaches of the synthesis of calcium carbonate micro-and nanoparticles, such as precipitation, slow carbonation, emulsion, polymer-mediated method, including in-situ polymerization, mechano-chemical, microwave-assisted method, and biological methods. The applications of these versatile calcium carbonate micro-and nanoparticles in the biomedical field (such as in drug delivery, therapeutics, tissue engineering, antimicrobial activity, biosensing applications), in industries, and environmental sector has also been comprehensively covered.

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Effect of the tryptone concentration on the calcium carbonate biomineralization mediated by Bacillus cereus

Cited by 8 publications

References 26 publications

Heavy metals bioremediation and water softening using ureolytic strains Metschnikowia pulcherrima and Raoultella planticola

Heavy metals bioremediation and water softening using ureolytic strains Metschnikowia pulcherrima and Raoultella planticola

Heavy Metals Bioremediation and Water Softening Using Ureolytic Metschnikowia Pulcherrima and Raoultella Planticola Strains

Calcium carbonate nano- and microparticles: synthesis methods and biological applications

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