Effect of the two-layer (University of Wisconsin solution???perfluorochemical plus O2) method of pancreas preservation on human islet isolation, as assessed by the Edmonton Isolation Protocol1

Abstract

In comparison with UW organ preservation, exposure of pancreata to the TLM result in greater islet yields and extended preservation times.

Chromatin-bound mitogen-activated protein kinases transmit dynamic signals in transcription complexes in β-cells

“…38 at the cGMP Islet Cell Processing Laboratory, Baylor Regional Transplant Institute. After procurement, pancreata were preserved by using a combination of University of Wisconsin solution and oxygenated perfluorodecalin (two-layer method) before islet isolation (39,40). The pancreatic duct was cannulated and initially perfused with cold collagenase enzyme NB1 (1.4 mg/ml) combined with neutral protease (SERVA Electrophoresis).…”

Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

“…In these experiments retrieved pancreases were shipped in UWS to the isolation facility, dissected to remove excessive fat and connective tissue and either immediately processed or oxygenated by TLM for an additional period of cold storage. Nevertheless, inspite of prolonged cold ischemia time significantly more islets could be isolated and recovered after culture compared to storage in UWS (Matsumoto et al, 2002a). These promising results could be confirmed in a similar setting by achieving a significantly higher islet transplantation rate of 71% in pancreases that were oxygenated for additionally 3 hours compared to UWS storage alone (36%) (Tsujimura et al, 2002a).…”

Tissue Engineering for Tissue and Organ Regeneration

“…However, the drawbacks of the Edmonton protocol include necessity of multiple donor organs, unstable islet isolation results, necessity of immunosuppressive drugs, difficulty of maintaining long-term insulin independence and severe shortage of donor organs . Currently, we introduced new pancreas preservation (Matsumoto et al, 2002a(Matsumoto et al, , 2002b(Matsumoto et al, , 2010b and islet isolation strategies (Noguchi et al, 2009a;Shimoda et al, 2010) and immunosuppressive therapy to improve the efficacy of islet isolation . Now, we have a very stable islet isolation method, in addition, a single donor pancreas is enough to achieve insulin independence .…”