Effect of thyroid state on enzymatic and non-enzymatic processes in H2O2 removal by liver mitochondria of male rats

Venditti, Paola; Napolitano, Gaetana; Barone, Daniela; Coppola, Ida; Meo, S. Di

doi:10.1016/j.mce.2015.01.019

Cited by 12 publications

(12 citation statements)

References 36 publications

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“…By contrast, the hormone treatment inhibited CYP2J19 expression in the same dosage range when administered alone. Thyroid hormones have been linked to high ROS generation and oxidative stress both in mammals and birds (reviewed in [33,[64][65][66]) due to increased oxidative metabolism (i.e. oxygen consumption rate) (e.g.…”

Section: Discussionmentioning

confidence: 99%

Testing the carotenoid-based sexual signalling mechanism by altering CYP2J19 gene expression and colour in a bird species

et al. 2020

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Ornaments can evolve to reveal individual quality when their production/maintenance costs make them reliable as ‘signals’ or if their expression level is intrinsically linked to condition by some unfalsifiable mechanism (indices). The latter has been mostly associated with traits constrained by body size. In red ketocarotenoid-based colorations, that link could, instead, be established with cell respiration at the inner mitochondrial membrane (IMM). The production mechanism could be independent of resource (yellow carotenoids) availability, thus discarding costs linked to allocation trade-offs. A gene coding for a ketolase enzyme (CYP2J19) responsible for converting dietary yellow carotenoids to red ketocarotenoids has recently been described. We treated male zebra finches with an antioxidant designed to penetrate the IMM (mitoTEMPO) and a thyroid hormone (triiodothyronine) with known hypermetabolic effects. Among hormone controls, MitoTEMPO downregulated CYP2J19 in the bill (a red ketocarotenoid-based ornament), supporting the mitochondrial involvement in ketolase function. Both treatments interacted when increasing hormone dosage, indicating that mitochondria and thyroid metabolisms could simultaneously regulate coloration. Moreover, CYP2J19 expression was positively correlated to redness but also to yellow carotenoid levels in the blood. However, treatment effects were not annulated when controlling for blood carotenoid variability, which suggests that costs linked to resource availability could be minor.

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Section: Discussionmentioning

confidence: 99%

Testing the carotenoid-based sexual signalling mechanism by altering CYP2J19 gene expression and colour in a bird species

et al. 2020

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“…However, the H 2 O 2 removal rates are similar with succinate or pyruvate/malate, substrates linked to complex II and I, respectively [ 251 ] (Venditti et al, 2014). The selective pharmacological inhibition of antioxidant enzymes shows that catalase is the major contributor to H 2 O 2 removal (~31%) and that TrxR and GPX also contribute significantly, but to a lesser extent (~20% and 23%, respectively) [ 252 ]. The contribution of non-enzymatic processes to H 2 O 2 removal is about 27% and appears to depend mainly on haemoproteins, as suggested by its changes in the mitochondria of animals with different cytochrome c content [ 252 ].…”

Section: Mitochondria Can Remove Ros Produced By Other Cellular Sourcesmentioning

confidence: 99%

Mitochondrial Management of Reactive Oxygen Species

Napolitano

Fasciolo²,

Venditti³

2021

Antioxidants

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102

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Mitochondria in aerobic eukaryotic cells are both the site of energy production and the formation of harmful species, such as radicals and other reactive oxygen species, known as ROS. They contain an efficient antioxidant system, including low-molecular-mass molecules and enzymes that specialize in removing various types of ROS or repairing the oxidative damage of biological molecules. Under normal conditions, ROS production is low, and mitochondria, which are their primary target, are slightly damaged in a similar way to other cellular compartments, since the ROS released by the mitochondria into the cytosol are negligible. As the mitochondrial generation of ROS increases, they can deactivate components of the respiratory chain and enzymes of the Krebs cycle, and mitochondria release a high amount of ROS that damage cellular structures. More recently, the feature of the mitochondrial antioxidant system, which does not specifically deal with intramitochondrial ROS, was discovered. Indeed, the mitochondrial antioxidant system detoxifies exogenous ROS species at the expense of reducing the equivalents generated in mitochondria. Thus, mitochondria are also a sink of ROS. These observations highlight the importance of the mitochondrial antioxidant system, which should be considered in our understanding of ROS-regulated processes. These processes include cell signaling and the progression of metabolic and neurodegenerative disease.

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“…9 Reduced glutathione is an important co-factor of both anti-oxidant enzymes and de-iodinases, the enzymes responsible for the conversion of iodine to iodide and T4 to T3. 24 Increase in MnSOD with decrease in GR in hyperthyroid rats has been experimented upon, advocating the increased lipid peroxidation and perhaps organ damage due to OS. We also observed similar results with increased MnSOD and low levels of GR produced in infertile females, which justifies association with an increase in TSH.…”

Section: Discussionmentioning

confidence: 99%

Role of oxidative stress and altered thyroid hormones in unexplained infertility

et al. 2020

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Objective: To explore the link between altered thyroid profile and oxidative stress marker in females with unexplained infertility. Methods: The cross-sectional case-control study was carried out at the Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan, from June 2016 to August 2017, and comprised women aged 18-40 years regardless of ethnic background who were divided into two groups; those with unexplained infertility were the cases, while fertile women acted as the controls. Serum was analysed for triiodothyronine, thyroxine and thyroid stimulating hormone as well as for oxidative stress markers including manganese superoxide dismutase, glutathione reductase and adrenaline using enzymelinked immunosorbent assay. Data was analysed using SPSS 19. Results: Of the 88 subjects, there were 44(50%) in each of the two groups. There was no significant difference in terms of thyroids markers except thyroxine and thyroid stimulating hormone (p<0.05). There were significant differences in terms of oxidative stress markers between the groups (p<0.05). A significant positive correlation of thyroid stimulating hormone was observed with manganese superoxide dismutase and adrenaline (p<0.05) with a weak non-significant association of glutathione reductase (p< (p>0.05). Conclusion: Increased thyroxine levels in females with unexplained infertility was associated with decrease in the serum levels of antioxidants.

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Effect of thyroid state on enzymatic and non-enzymatic processes in H2O2 removal by liver mitochondria of male rats

Cited by 12 publications

References 36 publications

Testing the carotenoid-based sexual signalling mechanism by altering CYP2J19 gene expression and colour in a bird species

Testing the carotenoid-based sexual signalling mechanism by altering CYP2J19 gene expression and colour in a bird species

Mitochondrial Management of Reactive Oxygen Species

Role of oxidative stress and altered thyroid hormones in unexplained infertility

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