Effect of Topical 2% Eucalyptus Extract on 5-FU-Induced Oral Mucositis in Male Golden Hamsters

Abstract

Oral mucositis is a common inflammatory complication among patients with cancer. This study evaluated the histopathological, stereological, and antioxidant markers of 2% eucalyptus extract in induced oral mucositis in male golden hamsters. In this animal study, oral mucositis was induced in 30 male golden hamsters by 5-FU (60 mg/kg) on days 0, 5, and 10 of the study. The cheek pouch was scratched with a sterile needle once daily on days 3 and 4. On days 14-17, 2% eucalyptus hydroalcoholic extract gel and Calendula officinalis extract gel groups were treated and then compared with a non-treated control group. The histopathological and stereological scores and the pouch content of malondialdehyde, as well as the activities of glutathione and myeloperoxidase in the pouch tissue, were evaluated. Histopathologic scores of oral mucositis were lower in the eucalyptus gel group than those of the calendula and control groups (p<0.05). Also, a lower malondialdehyde level and higher myeloperoxidase and glutathione activities were detected in the eucalyptus group in comparison to the calendula and control groups (p<0.001). The thickness of the mucosa and submucosa increased in the eucalyptus group. The numerical density of the fibroblast and the volume density of the collagen significantly increased in the eucalyptus group. In conclusion, the use of eucalyptus hydroalcoholic extract may be associated with reduced intensity of oral mucositis, diminished concentration of malondialdehyde, increased activity of myeloperoxidase and glutathione, increased volume of mucosa and submucosa, increased fibroblast and collagen in the induced oral mucositis in golden hamsters undergoing 5-FU consumption.

The effect of hydroalcoholic extract of Psidium guajava L. on experimentally induced oral mucosal wound in rat

“…Stereological evaluation of buccal mucosal tissue of the wounded area was performed according to previously reported procedures [ 41 ]. Briefly, for analyzing quantitative histomorphometric images the software (Stereo-Lite, SUMS, Shiraz, Iran) was used.…”

Diş Hekimliğinde Aromaterapi

“…37,47,48 Lactobasillus acidophilus gibi oral patojenlere karşı antibakteriyel etkisinin olduğunun saptanması okaliptusun antikaryojenik bir ajan olarak kullanılabileceğini göstermiştir. 49 Tanideh ve ark., 50 kanserli hastalarda sık görülen oral mukozit olgularında %2'lik okaliptus ekstraktının etkisini değerlendirmek amacı ile hamsterlarda bukkal bölgede 5-fluorourasilin ile oral mukozit oluşturmuşlar ve okaliptus ile tedavi edilen grupta mukoza ve submukoza kalınlığının, fibroblastlar ve kollajen yoğunluğunun anlamlı derecede arttığını saptamışlar, okaliptusun oral mukozit tedavisinde uygulanabilecek bir alternatif ajan olabileceğini öne sürmüşlerdir.…”

The chromatographic constitution of andiroba oil and his healing effects, compared to the LLLT outcomes, in oral mucositis induced in golden Syrian hamsters: a new treatment option

“…The use of phytotherapy shows satisfactory results in OM treatments, such as: chamomile [13][14][15], bilberry extract [16], Hydroalcoholic extract of Carumcarvi L [17], propolis [18] and eucalyptus hydroalcoholic extract [19]. Even though that herbal medicine is a very ancient practice, the studies related to the medical use of plants and its acceptance became more popular in the last years [20].…”