Effect of topical ozonetherapy on gingival wound healing in pigs: histological and immuno-histochemical analysis

Abstract

In this study, the effects of ozonetherapy on secondary wound healing were evaluated histologically and immuno-histochemically. Material and Methods: 8 healthy pigs were used in this study. Six wounds with 10 mm in diameter were created through the punch technique on the palatinal gingiva of each pig. Ozone gas was applied on only 3 wounds (test group) and the remaining 3 were left to natural healing (control group). Biopsy samples were taken from one of the wounds in each group on the third day, from another wound of each group on the seventh day, and from another one on the tenth day. Routine histological analysis and immuno-histochemical staining were performed to investigate transforming growth factor-beta (TGF-β) and (VEGF) expressions. Results: No statistical difference was found between the test and control groups in terms of collagen fibers, epithelial formation and inflammation scores. A VEGF expression found in the test group was statistically higher than control group samples taken on the 3rd and 7th day. There was no statistical difference between the test and control groups in terms of TGF-β expression on any of the sampling days. Conclusion: The topical application of ozone gas could be effective in the early stages of wound healing by increasing the amount of VEGF expression. Clinical Relevance: Topical application of ozone gas may be effective in the early stages of oral wound healing.

Ozone used as a supportive therapy for gingival tissue repair after facial trauma: Report of two cases

“…It was observed that ozone therapy could be effective in the early stages of repair with increased expression of the vascular endothelial growth factor. 11 Other authors have found improved repair of guinea pig skin wounds after using topical ozonated olive oil. 20 In an experimental study, a reduction in the amount of granulation tissue, lower number of inflammatory cells, and wider deposition of collagen type I were observed in Wistar rats with skin lesions that received 0.1 mL of subcutaneous ozone gas daily for 7 and 14 days.…”

“…5 Ozone therapy has been used and studied in several fields of health care. 6,7 In dentistry, some authors use ozone to assist the treatment of generalized chronic periodontitis, 8,9 in indirect dental pulp capping, 10 in oral wound repair, 11 in prosthesis-related traumatic ulcer pain relief, 12 and as an adjunct in the treatment of periimplantitis 13 and osteonecrosis of the jaws. 3 This report aimed to present two cases of facial trauma, with extensive damage to the gingival tissues, in which ozone was used as supportive therapy to help with tissue repair.…”

Clinical Uses and Molecular Aspects of Ozone Therapy: A Review

“…The mechanism of action of ozone therapy lays mainly in three aspects: Immunomodulation 2007), antimicrobial activity (Polydorou et al, 2012;Thanomsub et al, 2002) and the capacity to interplay with the oxidant/antioxidant balance (Soares et al, 2019). Altogether, another main aspect of the mechanism of the ozone treatment is the wound healing property and the main mechanism behind this is the capacity to interact with the skin epithelization system (Borges et al, 2017;Eroglu et al, 2018), as summarized in Fig. 1.…”

“…As well as increased vessel numbers and collagen deposition (Soares et al, 2019). Several endogenous growth factors, such as vascular Endothelial Growth Factor (VEGF), Transforming Growth Factor-beta (TGF-β), Fibroblast Growth Factor (FGF) and Platelet-Derived Growth Factor (PDGF), play an essential role in early wound healing (Eroglu et al, 2018), so it becomes crucial for the healing process the stimulation of the expression of these growth factors.…”

“…Oxygen-responsive species stimulate platelet aggregation and lead to the release of growth factors, that as mentioned above, play a key role in wound healing (Wang, 2018). According to recent researches, ozone increases endogenous growth factors (Eroglu et al, 2018) such as the expression of VEGF, TGF-β and PDGF, all involved in the cicatrization process (Zhang et al, 2014). Fibroblast Growth Factor 2 (FGF2), also known as basic FGF, is an essential protein that activates myofibroblastic angiogenesis-associated pathways and proliferation (Svystonyuk et al, 2015).…”