Effect of Two Strains of Probiotics on the Antioxidant Capacity, Oxidative Stress, and Immune Responses of Salmonella-Challenged Broilers

Abstract

The present study aimed at evaluating the effect of two strains of probiotics (Bacillus subtilis PB6 and Bacillus Cereus var. toyoi) on selected indicators of the antioxidant status and immune responses of broilers before and after challenge with Salmonella enterica subsp. Typhimurium (S. typhimurium). Birds were distributed into the following five treatments: negative control (CTL−); negative control+S. typhimurium (CTL+); antibiotic+S. typhimurium, (AB); Toyocerin®+S. typhimurium (TOYO); and CloSTAT TM + S. typhimurium (CLOS). Birds in all treatments, except for CTL−, were orally challenged with 3 x10 9 CFUS. typhimurium at 16 days of age. The results showed that total antioxidant capacity (TAC), superoxide dismutase activity (SOD), and hydrogen peroxide (H 2 O 2 ) values were similar among all groups. The antibody titers against S. typhimurium and Newcastle disease (ND) were not affected by the treatments (p>0.05). However, S. typhimurium and ND titers were influenced by the interaction between week and treatment (p<0.01, p<0.05, respectively) and by week (p<0.001, p<0.001, respectively). In conclusion, S. typhimurium challenge had an impact on anti-Salmonella and NC titers. Both probiotics did not affect the broilers' antioxidant capacity.

Impact of dietary Mannan-oligosaccharide and β-Glucan supplementation on growth, histopathology, E-coli colonization and hepatic transcripts of TNF-α and NF- ϰB of broiler challenged with E. coli O78

“…On the other hand, results of antioxidant enzymes and MDA in harmony with results of Zhou et al [15] who found significant decrease of antioxidant enzymes in birds infected with necrotic enteritis. On the other hand, Abudabos et al [16] reported that Salmonella typhimurium had no impact on total antioxidant capacity or oxidative stress in challenged broilers. However, agrimos® supplementation significantly decreased serum MDA and increased CAT.…”

The effects of the supplementation of multi‐strain probiotics on intestinal microbiota, metabolites and inflammation of young SPF chickens challenged with Salmonella enterica subsp. enterica

“…infection, but concerns regarding antimicrobial resistance have caused the European Union and many countries to restrict or ban the use of antibiotics (Toghyani, Toghyani, Gheisari, Ghalamkari, & Mohammadrezaei, ). In recent years, several studies have indicated that probiotics, prebiotics, and organic acids can function as antibacterial agents and protect chickens from Salmonella infection (Abudabos, Alyemni, & Zakaria, ; Chen, Tsen, Lin, Yu, & Chen, ; Fernández‐Rubio et al., ; Pourabedin, Chen, Yang, & Zhao, ). It has been suggested that probiotics exert beneficial effects on performance parameters, microbiota composition, and intestinal inflammation, potentially reducing the level of pathogen infection in the intestinal tract (Abudabos, Al‐Batshan, & Murshed, ; Chang, Teng, Lee, & Yu, ; Chen et al., ).…”

Effects of raw propolis or water and ethanol extracts of propolis on performance, immune system, and some blood parameters of broiler breeders

“…The total protein, albumen, triglyceride, Ca, P, aspartate aminotransferase (AST), and alanin aminotransferase (ALT) in plasma were analysed by an automatic analyser (Airone-200RA, Italy) using commercial kits. Total antioxidant (TAS) and total oxidant (TOS) amounts were determined by the ELISA method in plasma (Abudabos et al, 2016). The oxidative stress index was calculated by TOS:TAS ratio.…”