Effect of urease and nitrification inhibitors on ammonia volatilization and abundance of N‐cycling genes in an agricultural soil

Castellano‐Hinojosa, Antonio; González‐López, Jesús; Vallejo, Antonio; Bedmar, Eulogio J.

doi:10.1002/jpln.201900038

Cited by 29 publications

(15 citation statements)

References 56 publications

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“…Specifically, the AOB population exhibits a strong response to N fertilizers [48]. Shortly after the fertilizer application, soils tend to show a large increase in amoA abundance [39,49]. This growth can be avoided by applying SNIs such as DMPP, and the AOB population size is maintained at the level of unfertilized soils (Fig.…”

Section: Discussionmentioning

confidence: 99%

Biological and synthetic approaches to inhibiting nitrification in non-tilled Mediterranean soils

Bozal-Leorri

Corrochano-Monsalve

Arregui

et al. 2021

Chem. Biol. Technol. Agric.

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Background The increasing demand for food production has led to a tenfold increase in nitrogen (N) fertilizer use since the Green Revolution. Nowadays, agricultural soils have been turned into high-nitrifying environments that increase N pollution. To decrease N losses, synthetic nitrification inhibitors (SNIs) such as 3,4-dimethylpyrazole phosphate (DMPP) have been developed. However, SNIs are not widely adopted by farmers due to their biologically limited stability and soil mobility. On the other hand, allelopathic substances from root exudates from crops such as sorghum are known for their activity as biological nitrification inhibitors (BNIs). These substances are released directly into the rhizosphere. Nevertheless, BNI exudation could be modified or even suppressed if crop development is affected. In this work, we compare the performance of biological (sorghum crop) and synthetic (DMPP) nitrification inhibitors in field conditions. Results Sorghum crop BNIs and DMPP prevented an increase in the abundance of ammonia-oxidizing bacteria (AOB) without affecting the total bacterial abundance. Both nitrification inhibitors maintained similar soil NH4+ content, but at 30 days post-fertilization (DPF), the sorghum BNIs resulted in higher soil NO3− content than DMPP. Even so, these inhibitors managed to reduce 64% and 96%, respectively, of the NO3−-N/NH4+-N ratio compared to the control treatment. Similar to soil mineral N, there were no differences in leaf δ15N values between the two nitrification inhibitors, yet at 30 DPF, δ15N values from sorghum BNI were more positive than those of DMPP. N2O emissions from DMPP-treated soil were low throughout the experiment. Nevertheless, while sorghum BNIs also maintained low N2O emissions, they were associated with a substantial N2O emission peak at 3 DPF that lasted until 7 DPF. Conclusions Our results indicate that while sorghum root exudates can reduce nitrification in field soil, even at the same efficiency as DMPP for a certain amount of time, they are not able to prevent the N pollution derived from N fertilization as DMPP does during the entire experiment. Graphic Abstract

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Section: Discussionmentioning

confidence: 99%

Biological and synthetic approaches to inhibiting nitrification in non-tilled Mediterranean soils

Bozal-Leorri

Corrochano-Monsalve

Arregui

et al. 2021

Chem. Biol. Technol. Agric.

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“…4.21c and d). This result is the opposite to that of Castellano-Hinojosa et al (2019a), who suggested that the UI did not affect the copy number of the nosZ gene at 50% WFPS, but is in agreement with Shi et al (2017), who have observed a decrease in the abundance of nirK genes below a WFPS of 60%.…”

Section: Processes Involve In the N2o Productionsupporting

confidence: 72%

Strategies to mitigate N2O emissions and improve crop quality. Effect of Zn-N co-fertilization and N-enhanced efficiency fertilizers on microbial communities

Novillo¹

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XLV Sustainable agriculture aims to increase food production, in the context of a growing worldwide population, without compromising crop quality and yield whilst reducing environmental pollution. Most agricultural practices rely on external nitrogen (N) inputs, however, this N can be lost (e.g., through the release of the nitrous oxide (N2O) gas to the atmosphere) due to the activity of microbial communities that are involved in the N-cycle. New practices, therefore, need to be implemented in order to mitigate these N2O comparison with CAN. The inhibitor-based treatments led to a significant reduction of total N 2 O fluxes following a rewetting pulse, with respect to those of U or CAN. With regards to the N-cycling genes, the abundances of nitrifier and denitrifier communities, especially AOB, significantly decreased with the application of inhibitors with respect to the U or CAN treatments. From an agronomic point of view, CAN+DMPSA offers a further potential advantage related to crop quality: an increase in the oil yield. .

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“…To some extent, UI retarded the urea hydrolysis by decreasing urease activity and so slowed the conversion of urea-N to , which led to a slow-down in the reduction of to [ 37 , 38 ]. In our study, 80%U+UI decreased the urease activity between 15 and 45 DAF and decreased the nitrate reductase activity between 5 and 30 DAF ( Fig 2 ).…”

Section: Discussionmentioning

confidence: 99%

Adding NBPT to urea increases N use efficiency of maize and decreases the abundance of N-cycling soil microbes under reduced fertilizer-N rate on the North China Plain

Liu

Yang

et al. 2020

PLoS ONE

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Urease inhibitor (UI) and nitrification inhibitor (NI) can reduce N losses from agricultural soils but effects of inhibitors on N cycle are unclear. A field experiment was conducted with maize to test effects of UI (N-(n-Butyl) thiophosphoric, NBPT) and NI (3,4-dimethylepyrazolephosphate, DMPP) on N uptake and N-cycling soil microbes. Five treatments were imposed: no N fertilizer input (CK), conventional fertilization (CF) and 80% of urea input with NBPT (80%U+UI), with DMPP (80%U+NI) and with half NBPT and half DMPP (80%U+1/2(UI+NI)). There were no significant differences in biomass between 80%U+UI, 80%U+NI and CF but harvest index was increased under 80%U+UI and 80%U+NI. Compared to CF, N use efficiency of grain under 80%U+UI was increased by 7.1%, whereas grain yield and N uptake under 80%U+1/2(UI+NI) were decreased by 8.2% and 9.4%, respectively. The peak soil content was at about 15 days after fertilization (DAF) under CF but 30 DAF under the inhibitor treatments. In soils of 80%U+UI, the activities of urease and nitrate reductase were decreased between 15–45 DAF and between 5–30 DAF. The abundance of N-cycling soil microbes was affected: 80%U+UI and 80%U+NI reduced the copies of the amoA AOA and nir genes at about 15 days and reduced the copies of the amoA AOB gene at about 30 days. Correlation analysis indicated that there were significant positive relationships between amoA AOB gene and , as well as between nirK gene and . Overall, urea applied with NBPT has greater potential for improving maize N use efficiency and inhibiting nitrification under reduced fertilizer-N applications.

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Effect of urease and nitrification inhibitors on ammonia volatilization and abundance of N‐cycling genes in an agricultural soil

Cited by 29 publications

References 56 publications

Biological and synthetic approaches to inhibiting nitrification in non-tilled Mediterranean soils

Biological and synthetic approaches to inhibiting nitrification in non-tilled Mediterranean soils

Strategies to mitigate N2O emissions and improve crop quality. Effect of Zn-N co-fertilization and N-enhanced efficiency fertilizers on microbial communities

Adding NBPT to urea increases N use efficiency of maize and decreases the abundance of N-cycling soil microbes under reduced fertilizer-N rate on the North China Plain

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