Effect of Vaginal Lactobacillus Species on Escherichia coli Growth

Hudson, Patricia L.; Hung, Kristin J.; Bergerat, Agnès; Mitchell, Caroline

doi:10.1097/spv.0000000000000827

Cited by 22 publications

(10 citation statements)

References 14 publications

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“…For instance, we previously observed that Lactobacillus crispatus is frequently in the urobiomes of women with no LUTS ( Pearce et al, 2014 ). Prior studies have found that Lactobacilli can inhibit the growth of E. coli ( Atassi et al, 2006 , 2019 ; Cadieux et al, 2009 ; Hudson et al, 2020 ). In contrast, E. faecalis can promote E. coli growth under conditions for which it would otherwise not be able to thrive ( Keogh et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms

et al. 2020

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Urinary tract infections (UTIs) are one of the most common human bacterial infections. While UTIs are commonly associated with colonization by Escherichia coli , members of this species also have been found within the bladder of individuals with no lower urinary tract symptoms (no LUTS), also known as asymptomatic bacteriuria. Prior studies have found that both uropathogenic E. coli (UPEC) strains and E. coli isolates that are not associated with UTIs encode for virulence factors. Thus, the reason(s) why E. coli sometimes causes UTI-like symptoms remain(s) elusive. In this study, the genomes of 66 E. coli isolates from adult female bladders were sequenced. These isolates were collected from four cohorts, including women: (1) without lower urinary tract symptoms, (2) overactive bladder symptoms, (3) urgency urinary incontinence, and (4) a clinical diagnosis of UTI. Comparative genomic analyses were conducted, including core and accessory genome analyses, virulence and motility gene analyses, and antibiotic resistance prediction and testing. We found that the genomic content of these 66 E. coli isolates does not correspond with the participant’s symptom status. We thus looked beyond the E. coli genomes to the composition of the entire urobiome and found that the presence of E. coli alone was not sufficient to distinguish between the urobiomes of individuals with UTI and those with no LUTS. Because E. coli presence, abundance, and genomic content appear to be weak predictors of UTI status, we hypothesize that UTI symptoms associated with detection of E. coli are more likely the result of urobiome composition.

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Section: Discussionmentioning

confidence: 99%

Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms

et al. 2020

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“…The Lactobacillus species L. crispatus , L. gasseri , L. iners , and L. jensenii are predominant members of the “healthy” female urogenital microbiota ( 1 , 2 ). L. jensenii has been shown to be a protective species, reducing growth of uropathogenic Escherichia coli and sexually transmitted infections ( 3 – 6 ). Recently, Rocha et al ( 7 ) presented a new Lactobacillus species, L. mulieris .…”

Section: Observationmentioning

confidence: 99%

Comparative Genomic Study of Lactobacillus jensenii and the Newly Defined Lactobacillus mulieris Species Identifies Species-Specific Functionality

Putonti

Shapiro

Ene

et al. 2020

mSphere

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Lactobacilli are dominant members of the “healthy” female urogenital microbiota. One of these species, Lactobacillus jensenii, is routinely identified in the urinary microbiota of women both with and without urinary tract symptoms. In March 2020, the new bacterial species Lactobacillus mulieris was introduced, and phylogenetic and average nucleotide identity analysis identified eight L. jensenii strains that should be classified as members of the L. mulieris species. This prompted our phylogenomic study of all publicly available L. jensenii and L. mulieris genome sequences. While there is little variation in the 16S rRNA gene sequences, the core genome shows a clear distinction between genomes of the two species. We find eight additional strains of the species L. mulieris among these genomes. Furthermore, one strain, currently classified as L. mulieris UMB7784, is distinct from both L. jensenii and L. mulieris strains. As part of our comparative genomic study, we also investigated the genetic content that distinguishes these two species. Unique to the L. jensenii genomes are several genes related to catabolism of disaccharides. In contrast, L. mulieris genomes encode several cell surface and secreted proteins that are not found within the L. jensenii genomes. These L. jensenii-specific and L. mulieris-specific loci provide insight into phenotypic differences of these two species. IMPORTANCE Lactobacillus species play a key role in the health of the urinary tract. For instance, Lactobacillus crispatus and L. jensenii have been found to inhibit uropathogenic Escherichia coli growth. While L. crispatus is typically found only within the microbiota of women without lower urinary tract symptoms (LUTS), L. jensenii has been found in the microbiota of women both with and without LUTS. With the recent introduction of the new species Lactobacillus mulieris, several strains of L. jensenii were reclassified as L. mulieris based upon gene marker and average nucleotide identity. We took a phylogenomic and comparative genomic approach to ascertain the genetic determinants of these two species. Looking at a larger data set, we identified additional L. mulieris strains, including one distinct from other members of the species—L. mulieris UMB7784. Furthermore, we identified unique loci in each species that may have clinical implications.

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“…The common vaginal Lactobacillus species were used to investigate the inhibition of E. coli growth. The results showed that when L. crispatus was incubated with clinical E. coli strains, the growth of E. coli was inhibited in the acidic environment [112]. Wolff et al studied the changes in the ratio between uropathogens and Lactobacillus (U/L) within the lower UTI in response to oral probiotic supplementation.…”

Section: Probioticsmentioning

confidence: 99%

Deep insights into urinary tract infections and effective natural remedies

et al. 2021

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Background Urinary tract infection (UTI) is a common occurrence in females, during pregnancy, and in peri- and postmenopausal women. UTIs are associated with significant morbidity and mortality, and they affect the quality of life of the affected patients. Antibiotic therapy is an effective approach and reduces the duration of symptoms. Development of resistance, adverse effects of antibiotics, and other associated problems lead to establishing the research framework to find out the alternative approaches in controlling UTIs. Natural approaches have been extensively used for the management of various diseases to improve symptoms and also improve general health. Main body Different databases were employed to identify studies reporting on natural options including herbal medicines, vitamins, trace elementals, sugars, and probiotics without time limitations. Conclusion Herbal medicines can be effective at the first sign of the infection and also for short-term prophylaxis. Using vitamins, trace elementals, and/or sugars is an effective approach in preventing UTIs, and a combination of them with other antibacterial agents shows positive results. Probiotics have great potential for the threat of antibiotic over-usage and the prevalence of antibiotic-resistant microorganisms. This study may be of use in developing the efficient formulation of treatment of UTI.

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Effect of Vaginal Lactobacillus Species on Escherichia coli Growth

Cited by 22 publications

References 14 publications

Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms

Genomic Survey of E. coli From the Bladders of Women With and Without Lower Urinary Tract Symptoms

Comparative Genomic Study of Lactobacillus jensenii and the Newly Defined Lactobacillus mulieris Species Identifies Species-Specific Functionality

Deep insights into urinary tract infections and effective natural remedies

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