Effect of various substances on the colorimetric amino acid-ninhydrin reaction

D’Aniello, Antimo; D'Onofrio, Guiseppe; Pischetola, M.; Strazzullo, L

doi:10.1016/0003-2697(85)90160-5

Cited by 22 publications

(9 citation statements)

References 6 publications

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“…7). This was in agreement with previous work that showed plant derived extractives and lignins did not interfere with the ninhydrin reagent [14,20]. Thus, it appeared that the modified ninhydrin assay could be used to accurately and rapidly quantify total protein concentrations up to 800 μg/g in lignocellulosic biomass hydrolysis derived samples containing sugar concentration of up to 20 g/kg.…”

Section: Resultssupporting

confidence: 91%

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A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

Mok

Arantes

Saddler

2015

Appl Biochem Biotechnol

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Accurate protein quantification is necessary in many of the steps during the enzymatic hydrolysis of pretreated lignocellulosic biomass, from the fundamental determination of enzyme kinetics to techno-economic assessments, such as the use of enzyme recycling strategies, evaluation of enzyme costs, and the optimization of various process steps. In the work described here, a modified, more rapid ninhydrin-based protein quantification assay was developed to better quantify enzyme levels in the presence of lignocellulosic biomass derived compounds. The addition of sodium borohydride followed by acid hydrolysis at 130 °C greatly reduced interference from monosaccharides and oligosaccharides and decreased the assay time 6-fold. The modified ninhydrin assay was shown to be more accurate as compared to various traditional colorimetric protein assays when commercial cellulase enzyme mixtures were quantified under typical pretreated lignocellulosic biomass enzymatic hydrolysis conditions. The relatively short assay time and microplate-reading capability of the modified assay indicated that the method could likely be used for high-throughput protein determination.

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Section: Resultssupporting

confidence: 91%

“…In relative contrast, the ninhydrin assay has shown promise due to its specificity for amino acids and decreased susceptibility to interference [20][21][22][23][24]. However, high concentrations of sugars have been shown to be problematic, as were the long hydrolysis times typically required to attain complete protein hydrolysis [10,24].…”

Section: Introductionmentioning

confidence: 99%

A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

Mok

Arantes

Saddler

2015

Appl Biochem Biotechnol

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“…The trehalose content of cassava fresh leaves was measured using the anthrone-sulfuric acid colorimetric assay, whereby trehalose is generated into furfural in concentrated sulfuric acid under higher temperature, and the subsequent furfural reaction with anthrone forms a blue-green complex with maximum absorbance at 620 nm [38]. Proline was extracted with sulfosalicylic acid, and following a heat treatment, the proline and acidic-ninhydrin reaction generated a red substrate measured by absorbance at 520 nm [39]. Statistical analysis was verified by performing Student’s t -test ( p value < 0.05 was considered statistically significant).…”

Section: Methodsmentioning

confidence: 99%

Expression patterns of members of the ethylene signaling–related gene families in response to dehydration stresses in cassava

Ren¹,

Feng²,

Shi³

et al. 2017

PLoS ONE

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Drought is the one of the most important environment stresses that restricts crop yield worldwide. Cassava (Manihot esculenta Crantz) is an important food and energy crop that has many desirable traits such as drought, heat and low nutrients tolerance. However, the mechanisms underlying drought tolerance in cassava are unclear. Ethylene signaling pathway, from the upstream receptors to the downstream transcription factors, plays important roles in environmental stress responses during plant growth and development. In this study, we used bioinformatics approaches to identify and characterize candidate Manihot esculenta ethylene receptor genes and transcription factor genes. Using computational methods, we localized these genes on cassava chromosomes, constructed phylogenetic trees and identified stress-responsive cis-elements within their 5’ upstream regions. Additionally, we measured the trehalose and proline contents in cassava fresh leaves after drought, osmotic, and salt stress treatments, and then it was found that the regulation patterns of contents of proline and trehalose in response to various dehydration stresses were differential, or even the opposite, which shows that plant may take different coping strategies to deal with different stresses, when stresses come. Furthermore, expression profiles of these genes in different organs and tissues under non-stress and abiotic stress were investigated through quantitative real-time PCR (qRT-PCR) analyses in cassava. Expression profiles exhibited clear differences among different tissues under non-stress and various dehydration stress conditions. We found that the leaf and tuberous root tissues had the greatest and least responses, respectively, to drought stress through the ethylene signaling pathway in cassava. Moreover, tuber and root tissues had the greatest and least reponses to osmotic and salt stresses through ethylene signaling in cassava, respectively. These results show that these plant tissues had differential expression levels of genes involved in ethylene signaling in response to the stresses tested. Moreover, after several gene duplication events, the spatiotemporally differential expression pattern of homologous genes in response to abiotic and biotic stresses may imply their functional diversity as a mechanism for adapting to the environment. Our data provide a framework for further research on the molecular mechanisms of cassava resistance to drought stress and provide a foundation for breeding drought-resistant new cultivars.

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“…Several workers have introduced modifications to the method [1], the most noticeable ones being those by Moore and coworkers [8][9][10][11][12] and Doi et al [13]. The effect of metals and various other substances on this color reaction has also been studied by several workers [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

SYNTHESIS AND CHARACTERIZATION OF SOME METAL COMPLEXES OF A SCHIFF BASE DERIVED FROM NINHYDRIN AND α ,L-ALANINE

Derebe

Raju

Retta

2002

Bull. Chem. Soc. Eth.

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ABSTRACT. Complexes of Mn(II), Fe(III), Co(II), Ni(II) and Zn(II) with an intermediate Schiff base derived from ninhydrin and a,L-alanine (indane-1,3-dione-2-imine-N-2-propionate, IDIP) were successfully synthesized. All complexes were distinctly colored and were characterized by elemental analysis, molar conductance, magnetic susceptibility, infrared and electronic spectral studies. The ligand (Schiff base) was shown to behave as a monobasic tridentate ONO donor. The Mn(II) and Fe(III) complexes contain only one ligand molecule plus water and chloride(s) per metal ion, while all the others contain two ligand molecules per metal ion. An octahedral geometry is proposed for the metal complexes.A comparative anti-microbial study of the complexes was undertaken against two gramnegative bacteria, Escherichia coli and Proteus mirabilis and one gram-positive bacterium Staphylococcus aureus. It was revealed that most of the complexes and particularly Mn(II), Fe(III) and Co(II) complexes, have a much enhanced activity relative to ninhydrin against Proteus mirabilis and Staphylococcus aureus. All complexes showed a reduced activity against Escherichia coli.

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Effect of various substances on the colorimetric amino acid-ninhydrin reaction

Cited by 22 publications

References 6 publications

A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

Expression patterns of members of the ethylene signaling–related gene families in response to dehydration stresses in cassava

SYNTHESIS AND CHARACTERIZATION OF SOME METAL COMPLEXES OF A SCHIFF BASE DERIVED FROM NINHYDRIN AND α ,L-ALANINE

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Effect of various substances on the colorimetric amino acid-ninhydrin reaction

Cited by 22 publications

References 6 publications

A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

A NaBH4 Coupled Ninhydrin-Based Assay for the Quantification of Protein/Enzymes During the Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass

Expression patterns of members of the ethylene signaling–related gene families in response to dehydration stresses in cassava

SYNTHESIS AND CHARACTERIZATION OF SOME METAL COMPLEXES OF A SCHIFF BASE DERIVED FROM NINHYDRIN AND &alpha; ,L-ALANINE

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SYNTHESIS AND CHARACTERIZATION OF SOME METAL COMPLEXES OF A SCHIFF BASE DERIVED FROM NINHYDRIN AND α ,L-ALANINE