Effect of vitamin E and menadione supplementation on riboflavin production and stress parameters in Ashbya gossypii

Kavitha, S.; Chandra, T.S.

doi:10.1016/j.procbio.2009.04.001

Cited by 10 publications

(3 citation statements)

References 27 publications

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“…GTP cyclohydrolase is involved in riboflavin biosynthesis and in turn riboflavin protects cells from stress injuries (Perumal et al 2005; Sugiyama 1991) Elevated riboflavin is required for post-photoinductive events in sporulation of a Trichoderma auxotroph (Horwitz & Gressel 1983) and down regulation of this enzyme probably explains the reduced sporulation observed in TaDOR7316 during the heat stress. Similar results were also observed in A. nidulans and Ashbya gossypii exposed to oxidative stress agents (Pusztahelyi et al 2011; Kavitha & Chandra 2009). However, further studies are required to understand the exact role of these proteins in heat stress tolerance.…”

Section: Resultssupporting

confidence: 84%

Proteomic analysis reveals different sets of proteins expressed during high temperature stress in two thermotolerant isolates ofTrichoderma

Poosapati

Kumar

Ravulapalli

et al. 2021

Preprint

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Several species of the soil borne fungus of the genus Trichoderma are known to be versatile, opportunistic plant symbionts, and are the most successful biocontrol agents used in today's agriculture. To be successful in the field conditions, the fungus must endure varying climatic conditions. Studies have indicated that high atmospheric temperature coupled with low humidity is a major limitation for the inconsistent performance of Trichoderma under field conditions. Understanding the molecular modulation associated with such Trichoderma that persist and deliver under abiotic stress condition will aid in exploiting the worth of these organisms for such use. In this study, comparative proteomic analysis using two dimensional gel electrophoresis (2DE) and matrix assisted laser desorption/time of flight (MALDI-TOF-TOF) mass spectrometry was used to identify proteins associated with thermotolerance in two thermotolerant isolates of Trichoderma: T. longibrachiatum 673, TaDOR673 and T. asperellum 7316, TaDOR7316 and 32 differentially expressed proteins were identified. Sequence homology and conserved domains were used to identify these proteins and to assign probable function to them. Thermotolerant isolate, TaDOR673, seemed to employ the stress signaling MAPK pathways and heat shock response pathways to combat the stress condition whereas the moderately tolerant isolate, TaDOR7316, seemed to adapt to high temperature conditions by reducing the accumulation of misfolded proteins through unfolded protein response pathway and autophagy. Also, there were unique as well as common proteins that were differentially expressed in the two isolates studied.

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Section: Resultssupporting

confidence: 84%

Proteomic analysis reveals different sets of proteins expressed during high temperature stress in two thermotolerant isolates ofTrichoderma

Poosapati

Kumar

Ravulapalli

et al. 2021

Preprint

View full text Add to dashboard Cite

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“…Riboflavin overproduction starts when the A. gossypii growth rate declines and is characterized by a strong increase in the expression of three of these genes (AgRIB3, AgRIB4 and AgRIB5), which are regulated at the transcription level (Schlösser et al, 2001(Schlösser et al, , 2007Walther and Wendland, 2012). Environmental stress, such as nutritional (Schlösser et al, 2007) and oxidative stress (Kavitha and Chandra, 2009;Walther and Wendland, 2012), can trigger the overexpression of these genes and lead to riboflavin overproduction.…”

Section: Introductionmentioning

confidence: 99%

Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii

Silva

Aguiar

Domingues

2015

Journal of Biotechnology

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The Ashbya gossypii riboflavin biosynthetic pathway and its connection with the purine pathway have been well studied. However, the outcome of genetic alterations in the pyrimidine pathway on riboflavin production by A. gossypii had not yet been assessed. Here, we report that the blockage of the de novo pyrimidine biosynthetic pathway in the recently generated A. gossypii Agura3 uridine/uracil auxotrophic strain led to improved riboflavin production on standard agar-solidified complex medium. When extra uridine/uracil was supplied, the production of riboflavin by this auxotroph was repressed. High concentrations of uracil hampered this (and the parent) strain growth, whereas excess uridine favored the A. gossypii Agura3 growth. Considering that the riboflavin and the pyrimidine pathways share the same precursors and that riboflavin overproduction may be triggered by nutritional stress, we suggest that overproduction of riboflavin by the A. gossypii Agura3 may occur as an outcome of a nutritional stress response and/or of an increased availability in precursors for riboflavin biosynthesis, due to their reduced consumption by the pyrimidine pathway.

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“…the NADH pool is reduced as a result of low ATP demand [ 55 ]. This is especially interesting, because riboflavin biosynthesis in A. gossypii is associated with oxidative stress [ 56 , 57 ]. These studies provide evidence for an increased TCA cycle flux during growth on lipid-rich media, which might also apply for A. gossypii .…”

Section: Discussionmentioning

confidence: 99%

Metabolic flux analysis in Ashbya gossypii using 13C-labeled yeast extract: industrial riboflavin production under complex nutrient conditions

et al. 2018

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BackgroundThe fungus Ashbya gossypii is an important industrial producer of the vitamin riboflavin. Using this microbe, riboflavin is manufactured in a two-stage process based on a rich medium with vegetable oil, yeast extract and different precursors: an initial growth and a subsequent riboflavin production phase. So far, our knowledge on the intracellular metabolic fluxes of the fungus in this complex process is limited, but appears highly relevant to better understand and rationally engineer the underlying metabolism. To quantify intracellular fluxes of growing and riboflavin producing A. gossypii, studies with different 13C tracers were embedded into a framework of experimental design, isotopic labeling analysis by MS and NMR techniques, and model-based data processing. The studies included the use 13C of yeast extract, a key component used in the process.ResultsDuring growth, the TCA cycle was found highly active, whereas the cells exhibited a low flux through gluconeogenesis as well as pentose phosphate pathway. Yeast extract was the main carbon donor for anabolism, while vegetable oil selectively contributed to the proteinogenic amino acids glutamate, aspartate, and alanine. During the subsequent riboflavin biosynthetic phase, the carbon flux through the TCA cycle remained high. Regarding riboflavin formation, most of the vitamin’s carbon originated from rapeseed oil (81 ± 1%), however extracellular glycine and yeast extract also contributed with 9 ± 0% and 8 ± 0%, respectively. In addition, advanced yeast extract-based building blocks such as guanine and GTP were directly incorporated into the vitamin.ConclusionIntracellular carbon fluxes for growth and riboflavin production on vegetable oil provide the first flux insight into a fungus on complex industrial medium. The knowledge gained therefrom is valuable for further strain and process improvement. Yeast extract, while being the main carbon source during growth, contributes valuable building blocks to the synthesis of vitamin B2. This highlights the importance of careful selection of the right yeast extract for a process based on its unique composition.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1003-y) contains supplementary material, which is available to authorized users.

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Effect of vitamin E and menadione supplementation on riboflavin production and stress parameters in Ashbya gossypii

Cited by 10 publications

References 27 publications

Proteomic analysis reveals different sets of proteins expressed during high temperature stress in two thermotolerant isolates ofTrichoderma

Proteomic analysis reveals different sets of proteins expressed during high temperature stress in two thermotolerant isolates ofTrichoderma

Blockage of the pyrimidine biosynthetic pathway affects riboflavin production in Ashbya gossypii

Metabolic flux analysis in Ashbya gossypii using 13C-labeled yeast extract: industrial riboflavin production under complex nutrient conditions

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