Effect of Vitamin E on Keratinocyte-Modulation Induced by Lauroylsarcosine

Abstract

ABSTRACT-The effect of vitamin E on the modulation of keratinocytes was studied in rats. A 1°10 lauroylsarcosine (LS) ointment caused skin erythema with keratinocyte-damage. A 30% vitamin E ointment markedly alleviated this erythema and protected keratinocytes from cell damage. Vitamin E (100 pg/ml) was also effective on LS (7.5 pg/ml)-induced proliferative reduction of cultured keratinocytes. On the other hand, ointment containing superoxide dismutase (SOD) (99,000 U/g) decreased the LS-induced erythema, suggesting that superoxide anion (02) produced from keratinocytes play an important role in the skin irritation. Indeed, LS induced 02-production from cultured keratinocytes. The 02-was significantly reduced by vitamin E and SOD, although vitamin E had no effects on 02-production in a xanthine-xanthine oxidase system, unlike the effect observed with SOD. These results indicate that vitamin E is an inhibitor of keratinocyte-modulation.

Vitamin E ointment at high dose levels suppresses contact dermatitis in rats by stabilizing keratinocytes

“…The same was true for 0.5 % prednisolone ointment. The vitamin E acetate ointment also evidently alleviated LSinduced skin irritation, probably a response initiated by keratinocyte modulation, was reported previously [17]. Vol.…”

“…51 (2002) 483 -489 1023-3830/02/100483-07 stimuli including toxic chemicals and contact allergens are postulated to initiate contact dermatitis through modulation of epidermal keratinocytes [8][9][10], and thereby amplify the inflammatory response in atopic dermatitis and psoriasis [11][12][13][14][15][16]. We reported previously that topical vitamin E alleviated skin irritation induced by a transdermal absorption enhancer and that its possible mechanism was the inhibition of keratinocyte-modulation but not of the catalyzing action of O 2 - [17]. In the present study, according to the postulation mentioned above, we investigated the effect of vitamin E ointment at high dose levels on allergic and irritant contact dermatitis.…”