Effect of water extracts from Cynanchum thesioides (Freyn) K. Schum. on visceral hypersensitivity and gut microbiota profile in maternally separated rats

Ling-peng, Pei; Jingzhu, Song; Liu, Wei; Enqi, Wu; Ling, Yaqin

doi:10.1016/j.jep.2020.113352

Cited by 11 publications

(8 citation statements)

References 34 publications

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“…In contrast to its components, the functionalities of the lung microbiota seem to be less variable and more stable. A total of 284 KEGG pathways were enriched from the lung microbiota in the present study—a number comparable to the 258 KEGG pathways enriched from the gut microbiota data in another study 29 .…”

Section: Discussionsupporting

confidence: 77%

Dynamic alterations in the lung microbiota in a rat model of lipopolysaccharide-induced acute lung injury

Tian

Enqi

You

et al. 2022

Sci Rep

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The lung microbiota have been found to be substantially altered in numerous pulmonary disorders, and crosstalk between the host pathophysiology and lung microbiota plays critical roles in the regulation of disease states. The aim of this study was to investigate dynamic changes in the lung microbiota during different stages of acute lung injury and acute respiratory distress syndrome (ALI/ARDS). Rats receiving an intraperitoneal administration of lipopolysaccharide (LPS) were sacrificed at 12 and 48 h after injection, and the hematological parameters, serum cytokine levels, and histological characteristics of the lung tissue and lung microbiota were assessed. After LPS injection, along with fluctuations of systemic cytokine levels and the onset and regression of pulmonary edema, the diversity, components, and functionalities of the pulmonary microbiota underwent significant dynamic changes. The volatility of the α-diversity indices narrowed after LPS injection, and the indices significantly decreased 48 h later. The abundance of 18 genera and functionality of adenosine triphosphate–binding cassette (ABC) transporters, pentose phosphate, and bacterial chemotaxis pathways were found to significantly differ between specified time points. Several significant correlations between the components and functionalities of the lung microbiota and indicative symptoms of ALI/ARDS were also observed. Brevibacterium was correlated with cytokines tumor necrosis factor (TNF)-α, interleukin (IL)-10, and IL-6 and with hematological percentage of neutrophils (NEU%); Wnt, Notch, and chronic myeloid leukemia signaling pathways were correlated with IL-1β; mitogen-activated protein kinase (MAPK) signaling pathway–yeast was correlated with IL-10; and the pathways of ascorbate and aldarate metabolism and basal transcription factors were correlated with platelet-related indicators. The correlations between the lung microbiota and indicative symptoms of ALI/ARDS identified in this study support further investigation into the underlying mechanism of host–microbiota interactions during lung injury and repair.

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Section: Discussionsupporting

confidence: 77%

Dynamic alterations in the lung microbiota in a rat model of lipopolysaccharide-induced acute lung injury

Tian

Enqi

You

et al. 2022

Sci Rep

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“…However, the molecular mechanisms remain to be elucidated. Dysbiosis in the commensal bacterial communities, increased intestinal permeability, irregular intestinal motility, visceral hypersensitivity, and anxiety/depression-like behaviors have been observed in animal models ( Labus et al, 2017 ; Fukui et al, 2018 ; Cojocariu et al, 2020 ; Sugiyama and Shiotani, 2020 ; Lingpeng et al, 2021 ). Accumulating evidence indicates that ECs play a crucial role in the pathogenesis of ELS-induced IBS ( Bian et al, 2011 ; Chow et al, 2019 ; Qin et al, 2019a ; Wong et al, 2019 ) as ECs bidirectionally (top-down or down-top) mediated signaling transmission of the brain–gut–microbiota axis ( Stasi et al, 2012 ).…”

Section: Evidence For Early Life Stress-induced Irritable Bowel Syndromementioning

confidence: 99%

“…Furthermore, ELS is correlated with the severity of IBS symptoms ( Park et al, 2016 ). However, the precise molecular mechanisms by which ELS induces IBS remain unclear, and dysbiosis in the composition of commensal bacterial communities, increased intestinal permeability, irregular intestinal motility, visceral hypersensitivity, and anxiety/depression-like behaviors may be implicated ( Labus et al, 2017 ; Fukui et al, 2018 ; Cojocariu et al, 2020 ; Sugiyama and Shiotani, 2020 ; Lingpeng et al, 2021 ). Recently, studies have shown that ELS increases susceptibility to IBS in later life ( Wong et al, 2019 ; Low et al, 2020 ), which is associated with an increased number of ECs ( Wong et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Potential Roles of Enterochromaffin Cells in Early Life Stress-Induced Irritable Bowel Syndrome

Tao

Zhu

et al. 2022

Front. Cell. Neurosci.

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Irritable bowel syndrome (IBS) is one of the most common functional gastrointestinal disorders, also known as disorders of the gut–brain interaction; however, the pathophysiology of IBS remains unclear. Early life stress (ELS) is one of the most common risk factors for IBS development. However, the molecular mechanisms by which ELS induces IBS remain unclear. Enterochromaffin cells (ECs), as a prime source of peripheral serotonin (5-HT), play a pivotal role in intestinal motility, secretion, proinflammatory and anti-inflammatory effects, and visceral sensation. ECs can sense various stimuli and microbiota metabolites such as short-chain fatty acids (SCFAs) and secondary bile acids. ECs can sense the luminal environment and transmit signals to the brain via exogenous vagal and spinal nerve afferents. Increasing evidence suggests that an ECs-5-HT signaling imbalance plays a crucial role in the pathogenesis of ELS-induced IBS. A recent study using a maternal separation (MS) animal model mimicking ELS showed that MS induced expansion of intestinal stem cells and their differentiation toward secretory lineages, including ECs, leading to ECs hyperplasia, increased 5-HT production, and visceral hyperalgesia. This suggests that ELS-induced IBS may be associated with increased ECs-5-HT signaling. Furthermore, ECs are closely related to corticotropin-releasing hormone, mast cells, neuron growth factor, bile acids, and SCFAs, all of which contribute to the pathogenesis of IBS. Collectively, ECs may play a role in the pathogenesis of ELS-induced IBS. Therefore, this review summarizes the physiological function of ECs and focuses on their potential role in the pathogenesis of IBS based on clinical and pre-clinical evidence.

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“…Of note, SHE diminished CRD scores to the same extent as gabapentin, one of the most frequently prescribed drugs against chronic neuropathic pain and for visceral hyperalgesia in human IBS (Trinkley and Nahata, 2014) (Figure 2). Indeed, other plant extracts or plantderived natural compounds, like quercetin, have ameliorated visceral hypersensitivity in other experimental models of IBS as well as in vitro assays (Qin et al, 2019;Lingpeng et al, 2021). This data could support the potential future use of SHE in visceral hyperalgesia in human IBS.…”

Section: Contraction Of Abdominal Musclesmentioning

confidence: 63%

Intestinal anti-inflammatory and visceral analgesic effects of a Serpylli herba extract in an experimental model of irritable bowel syndrome in rats

et al. 2022

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Ethnopharmacological relevance:Serpylli herba extract (SHE), composed of the aerial parts of wild thyme (Thymus serpyllum L.) (Lamiaceae family), is traditionally used in Europe and North Africa to treat diarrhea, gastric ulcers, intestinal parasites and upper respiratory tract infections. Recently, SHE has generated a great interest for irritable bowel syndrome (IBS) management, probably due to its intestinal anti-inflammatory properties shown in experimental colitis and the fact that its active components could preserve the intestinal barrier integrity, which is altered in patients with IBS.Aim of study: We aimed to test the effects of a SHE in a rat experimental model resembling human IBS.Materials and methods: IBS was provoked by deoxycholic acid (DCA). Rats were then treated with SHE (100 mg/kg) or gabapentin (70 mg/kg) and different inflammatory and gut barrier integrity markers were evaluated. Moreover, several gut hypersensitivity and hyperalgesia determinations were performed.Results: SHE improved referred pain and visceral hypersensitivity. Additionally, SHE enhanced immune status by downregulating of the expression of the pro-inflammatory mediators Il-1β, Il-6, Ifn-γ, Tlr-4, and the inducible enzyme Cox-2, thus inducing visceral analgesia, and promoting the restore of the gut barrier function by upregulating the mucins Muc-2 and Muc-3. These anti-inflammatory effects could be related to its action on mast cells since it significantly inhibited the β-Hexosaminidase production in RBL-2H3 cells. Lastly, SHE also seems to modulate the serotonin pathway by restoring the altered expression of the 5-HT receptors Htr-3 and Htr-4.Conclusion: SHE could be considered a potential new treatment for IBS, since it ameliorates hypersensitivity, visceral hyperalgesia, and inflammation. These beneficial effects may be due to the inhibition of mast cells degranulation and serotonin pathway.

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Effect of water extracts from Cynanchum thesioides (Freyn) K. Schum. on visceral hypersensitivity and gut microbiota profile in maternally separated rats

Cited by 11 publications

References 34 publications

Dynamic alterations in the lung microbiota in a rat model of lipopolysaccharide-induced acute lung injury

Dynamic alterations in the lung microbiota in a rat model of lipopolysaccharide-induced acute lung injury

Potential Roles of Enterochromaffin Cells in Early Life Stress-Induced Irritable Bowel Syndrome

Intestinal anti-inflammatory and visceral analgesic effects of a Serpylli herba extract in an experimental model of irritable bowel syndrome in rats

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