Effect of weak static and low‐frequency alternating magnetic fields on the fission and regeneration of the planarian dugesia (Girardia) tigrina

Novikov, V. V.; Sheiman, I. M.; Фесенко, Е. Е.

doi:10.1002/bem.20407

Cited by 46 publications

(20 citation statements)

References 9 publications

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“…Exposure to electric or magnetic fields is reported to affect cell function in a variety of ways depending upon exposure and pulse design (Gordon, 2006; 2008; Jenrow et al 1995 Jenrow et al 1996; Novikov et al 2008; Albertini et al 1999; DiCarlo et al 1999; Shallom et al 2002; George et al 2008). While generally efficacious, the most effective exposure conditions for a specific type of repair could depend on such variables as the magnetic and electric field components (dB/dt), signal modulation (sine waves versus pulsing fields) versus unmodulated sine waves, etc.…”

Section: Discussionmentioning

confidence: 99%

Extremely low frequency electromagnetic fields activate the ERK cascade, increase hsp70 protein levels and promote regeneration in Planaria

Goodman¹,

Lin-Ye²,

Geddis³

et al. 2009

Int. J. of Radiation Biol.

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Purpose-To use regenerating Planaria Dugesia dorotocethala as a model to determine whether an intermittent modulated extremely low frequency electro-magnetic field (ELF-EMF) produces elevated levels of the heat shock protein hsp70 and stimulates intracellular pathways known to be involved in injury and repair. We focused on serum response element (SRE) binding through the extra-cellular signal-regulated kinase (ERK) cascade.Materials and methods-Planaria were transected equidistant between the tip of the head and the tip of the tail. Individual head and tail portions from the same worm were exposed to a 60 Hertz 80 milliGauss ELF-EMF for one hour twice daily for 15 days post transection under carefully controlled exposure conditions. The regenerating heads and tails were photographed and the lengths measured at 3-day intervals. In other experiments, the timing of the appearance of pigmented eyes was monitored in the tail portion at 12 hour intervals following transection in both ELF-EMF exposed and sham control. In some experiments protein lysates were analyzed for hsp70 levels, doubly phosphorylated (pp)-ERK, Elk-1 kinase activity and serum response factor (SRF) -SRE binding.Results-ELF-EMF exposure during the initial 3-days post surgery caused a significant increase in regeneration for both heads and tails, but especially tails. The first appearance of eyes occurred at day seven post-transection in tail portions exposed to ELF-EMF. In the sham control tail samples the initial appearance of eyes occurred 48 hours later. Concurrently, ELF-EMF-exposed heads and tails exhibited an elevation in the level of hsp70 protein, an activation of an ERK cascade, and an increase in SRF-SRE binding.Conclusion-Exposures to a modulated sinusoidal ELF-EMF were delivered by a Helmholtz configuration at a frequency of 60Hz and 80mG twice a day for one hour. This is accompanied by * Correspondence to: Reba Goodman, 212 305 3646 (Voice), rmg5@columbia.edu. NIH Public Access Author ManuscriptInt J Radiat Biol. Author manuscript; available in PMC 2010 December 9. an increase in hsp70 protein levels, activation of specific kinases and up-regulation of transcription factors that are generally associated with repair processes.

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Section: Discussionmentioning

confidence: 99%

Extremely low frequency electromagnetic fields activate the ERK cascade, increase hsp70 protein levels and promote regeneration in Planaria

Goodman¹,

Lin-Ye²,

Geddis³

et al. 2009

Int. J. of Radiation Biol.

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“…Planarian capacity to regenerate and multiply asexually [9] is influenced by weak (∼10 µT) power frequency magnetic fields [10] and frequencies tuned to calcium resonance [11] which is a likely mechanism for membrane voltage-mediated changes in anterior gene expression [12]. Intensities as low as 40 nT and a variety of frequencies such as 1 Hz, 3 Hz, 7 Hz, 32 Hz and 60 Hz can stimulate fission [13].…”

Section: Introductionmentioning

confidence: 99%

Temporally-Patterned Magnetic Fields Induce Complete Fragmentation in Planaria

et al. 2013

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A tandem sequence composed of weak temporally-patterned magnetic fields was discovered that produced 100% dissolution of planarian in their home environment. After five consecutive days of 6.5 hr exposure to a frequency-modulated magnetic field (0.1 to 2 µT), immediately followed by an additional 6.5 hr exposure on the fifth day, to another complex field (0.5 to 5 µT) with exponentially increasing spectral power 100% of planarian dissolved within 24 hr. Reversal of the sequence of the fields or presentation of only one pattern for the same duration did not produce this effect. Direct video evidence showed expansion (by visual estimation ∼twice normal volume) of the planarian following the first field pattern followed by size reduction (estimated ∼1/2 of normal volume) and death upon activation of the second pattern. The contortions displayed by the planarian during the last field exposure suggest effects on contractile proteins and alterations in the cell membrane’s permeability to water.

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“…However, the mechanism or mechanisms of action remain an open question [Eremenko et al, 1997;Harland et al, 1999;Cook et al, 2002;Barnes, 2006]. More recently, accumulating proof that some configurations of low-level magnetic fields have effects on different kinds of cells [Simko et al, 2001;Del Re et al, 2004;Ravera et al, 2004;Novikov et al, 2008], tissues [Barnes, 2006;Akdag et al, 2007], and organisms of different complexity [Lednev et al, 1996;Berk et al, 1997;Thomas et al, 1998;Prato et al, 2005;Barnes, 2006;Martino et al, 2010] have become hard to ignore.…”

Section: Introductionmentioning

confidence: 99%

Reduction of the earth's magnetic field inhibits growth rates of model cancer cell lines

Martino

Portelli

McCabe

et al. 2010

Bioelectromagnetics

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Small alterations in static magnetic fields have been shown to affect certain chemical reaction rates ex vivo. In this manuscript, we present data demonstrating that similar small changes in static magnetic fields between individual cell culture incubators results in significantly altered cell cycle rates for multiple cancer-derived cell lines. This change as assessed by cell number is not a result of apoptosis, necrosis, or cell cycle alterations. While the underlying mechanism is unclear, the implications for all cell culture experiments are clear; static magnetic field conditions within incubators must be considered and/or controlled just as one does for temperature, humidity, and carbon dioxide concentration.

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Effect of weak static and low‐frequency alternating magnetic fields on the fission and regeneration of the planarian dugesia (Girardia) tigrina

Cited by 46 publications

References 9 publications

Extremely low frequency electromagnetic fields activate the ERK cascade, increase hsp70 protein levels and promote regeneration in Planaria

Extremely low frequency electromagnetic fields activate the ERK cascade, increase hsp70 protein levels and promote regeneration in Planaria

Temporally-Patterned Magnetic Fields Induce Complete Fragmentation in Planaria

Reduction of the earth's magnetic field inhibits growth rates of model cancer cell lines

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