Effect of κ-Casein Polymorphism on Milk Composition in the Orobica Goat

Chiatti, F.; Chessa, S.; Bolla, P.; Cigalino, G.; Caroli, Anna; Pagnacco, G.

doi:10.3168/jds.2006-508

Cited by 38 publications

(27 citation statements)

References 18 publications

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“…However, the B allele of j-CN was found to be significantly favourable for milk and protein yields. And for protein percentage, the B allele was reported to be to be significantly associated with milk yield and composition traits which are correlated with cheese-yielding efficiency in cattle and goat breeds (Chiatti et al 2007). In contrast to j-CN gene, b-LG gene exhibits three genotypes in many different cattle breeds (Tsiaras et al 2005;Patel and Chauhan 2008;Akyuz et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Genotyping of the kappa‐casein and beta‐lactoglobulin genes in Anatolian water buffalo by PCR‐RFLP

Çınar

Akyüz

Arslan

et al. 2015

Int J of Dairy Tech

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The objective of this study was to identify allele and genotype frequencies of the j-CN and b-LG genes in Anatolian water buffalo. A total of 126 water buffalos from Turkey were genotyped using the PCR-RFLP method. For gene j-CN, only B allele and BB genotype were observed. And for gene b-LG, two types of alleles (A and B) and three types of genotypes were observed. The genotype frequencies of AA, AB and BB of b-LG in Anatolian water buffalo were 0.254, 0.698 and 0.048, respectively. Surprisingly, the frequency of allele A was higher than that of allele B in contrast to world buffalo breeds.

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Section: Discussionmentioning

confidence: 99%

Genotyping of the kappa‐casein and beta‐lactoglobulin genes in Anatolian water buffalo by PCR‐RFLP

Çınar

Akyüz

Arslan

et al. 2015

Int J of Dairy Tech

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“…Prinzenberg et al (2005), proposed to differentiate the nomenclature at protein level from the one used at DNA level introducing two codes (A IEF and B IEF ) corresponding to two IPs (IP=5.53 and 5.78, respectively) identified using isoelectrofocusing (IEF) method (Table 1). According to this nomenclature, among the CSN3 alleles found in our study, only the D and D' alleles are included in B IEF group, whereas A, B, G and N alleles belong to A IEF group (Table 1), which represents the less favourable variants group in terms of milk composition and technological properties (Chiatti et al, 2007).…”

Section: Identified Alleles In Girgentana Goat Breedmentioning

confidence: 97%

Molecular Characterisation ofκ–CaseinGene inGirgentanaDairy Goat Breed and Identification of Two New Alleles

Gerlando

Tortorici

Sardina

et al. 2015

Italian Journal of Animal Science

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The k-casein fraction plays an important role in the formation, stabilisation and aggregation on casein micelles and thus affects technological and nutritional properties of milk. In this study, exon 4 of k-casein (CSN3) gene was sequenced and analysed in Girgentana goat breed. Analyses of the obtained sequences showed the presence of A, B, D, and G known alleles and two new genetic variants, named D' and N. The new D' allele differs from D in one transition, G284→A284, which did not cause amino acid change. The new N allele differs from A in five single nucleotide polymorphisms (SNPs): T245/C245, G284/A284, G309/A309, G471/A471 and T591/C591, while it differs from C in one transition, i.e. T583→C583. Comparing the amino acid sequences of N and A alleles, the first two SNPs caused no amino acid change, whereas the other SNPs produced changes (Val65/Ile65, Val119/Ile119, and Ser159/Pro159, respectively). Comparison of N allele with C revealed the amino acid change Val156→Ala156. The most frequent allele was A (0.480) followed by B (0.363), D (0.112), and N (0.034). The D' and G alleles were identified only in two animals and in heterozygous conditions with a very low frequency (0.005). The most common genotype was AB (39.5%) followed by AA (19.5%), AD (12.7%), and BB (11.7%). Homozygous D'D', GG, and NN individuals were not found. Further analysis will be performed in order to establish associations among genotypes and quantitative and qualitative milk traits.

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“…The B, B , and H alleles all belong to the AIEF variant, which is the less favourable -casein variant in terms of milk composition and technological properties (Haenlein, 2002;Chiatti et al, 2007).…”

Section: Discussionmentioning

confidence: 99%