Effect on Histology and Nutrient Digestibility of Supplemented Origanum onites Essential Oil to Rainbow Trout Diets (Oncorhynchus mykiss)

Abstract

The present experiment was conducted to describe the effects on histology (experiment 1) and nutrient digestibility (experiment 2) of rainbow trout fed with diet containing O. onites oil. Diets were prepared with supplemented in different concentrate (0, 0.125, 1.5, 2.5, 3.0 ml kg -1 ) of O. onites oil. A total of 1050 trout (body weight 26.05 ± 0.15 g) were randomly stocked into 15 tanks. The experiments were done in triplicate.In the experiment 1, rainbow trout were fed during 90 day for histology analysis. Five fish samples from per tank were taken at the end of the experiment. Liver and kidney histopathology of the control fish showed a normal histological architect without indication of any deformity. Slightly necrosis or degeneration were observed in liver and kidney samples of fish fed with high level of O. onites oil in diets. In the experiment 2, nutrient digestibility study was conducted for 2 week in following of the histology experiment. Nutrient digestibility was determined using 0.5 % Cr 2 O 3 as an indicator to diet. Feces were collected by siphon from bottom of tanks during 2 week. The end of the experiment, dry matter, protein and lipid digestibility did not affect with supplemented in different concentrate of O. onites oil to trout diet.

Effects of Adding Laurel (Laurus nobilis) Essential Oil to the Diet of Tilapia Fish on Growth and Intestinal Histology

“…In our study, vacuolization was observed in hepatocyte cells with the addition of laurel oil. Similar to our study, it was observed in tambaqui fry-fed 1.0 mL kg -1 ginger EO (Chung et al, 2021) and rainbow trout fed with Origanum onites essential oil (Yigit et al, 2017). However, in the tilapias examined, no pathological picture such as necrosis was found in hepatocyte cells reported by these researchers.…”

“…Intestinal anatomy and histology are very important for nutritional evaluation and immunological functions in fish health. Therefore, histological examination of the gut can be considered a potential indicator of the overall health of the fish and the efficacy of feed additives (Yigit et al, 2017;Abdel-Latif et al, 2021;Acar et al, 2021).…”

Effects of Essential Oil Derived from the Bitter Orange (Citrus aurantium) on Growth Performance, Histology and Gene Expression Levels in Common Carp Juveniles (Cyprinus carpio)

“…Histopathological experiments play an important role in exposing improvements in fish tissues and cells during adverse circumstances [ 26 , 27 ]. Antioxidant, anti-inflammatory, and essential oils derived from plants are used as feed additives because of the characteristics in the fish diets [ 28 , 29 , 30 ]. The liver is largely responsible for lipid metabolism in fish, which involves both fatty acid production and oxidation.…”

“…Yilmaz [ 31 ] discovered that high-fat diets trigger liver lipoid degeneration (steatosis) in carp ( Cyprinus carpio ). In addition, hepatocyte cytoplasmic vacuolation has been observed in the livers of rainbow trout fed with Origanum onites essential oil-supplemented (2.5 mL kg −1 ) diets for 90 days [ 28 ]. The liver histopathological examination in the current study did not reveal any significantly differences in lesions other than lipid vacuole accumulation between the experimental groups ( p > 0.05).…”

Dietary supplementation with Lippia sidoides essential oil improves organ integrity but the specific activity of antioxidant enzymes is dose‐dependent in Danio rerio

“…However, Yigit et al (2017) reported necrosis or degeneration in liver of rainbow trout O. mykiss fed a diet containing Origanum onites essential oil at the highest concentrations (1.5, 2.5 and 3 mL kg -1 ). At the time, the researchers highlighted that in high concentrations O.…”