Effective cell separation utilizing poly(N-isopropylacrylamide)-grafted polypropylene membrane containing adsorbed antibody

Okamura, Ai; Hagiwara, Taeko; Yamagami, Souko; Yamaguchi, Manae; Shinbo, Toshio; Kanamori, Toshiyuki; Kondo, Satoshi; Miwa, Keishi; Itagaki, Ichiro

doi:10.1263/jbb.105.221

Cited by 10 publications

(5 citation statements)

References 10 publications

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“…While osteoblast cells adhere onto this surface and proliferate therein at 37 °C, the cell sheet detaches from the surface when the temperature is lowered to 25 °C, which avoids the need for an enzymatic agent 108. Similar work was also reported by Okamura and co‐workers, in which effective cell separation could be realized by utilizing PNIPAAm‐grafted polypropylene membrane containing absorbed antibody 109. In other works, the influence of PNIPAAm film thickness on the cell adhesion or protein absorption was also investigated carefully 110…”

Section: Biologically Related Applicationssupporting

confidence: 62%

Biomimetic Smart Interface Materials for Biological Applications

2011

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Controlling the surface chemical and physical properties of materials and modulating the interfacial behaviors of biological entities, e.g., cells and biomolecules, are central tasks in the study of biomaterials. In this context, smart polymer interface materials have recently attracted much interest in biorelated applications and have broad prospects due to the excellent controllability of their surface properties by external stimuli. Among such materials, poly(N-isopropylacrylamide) and its copolymer films are especially attractive due to their reversible hydrogen-bonding-mediated reversible phase transition, which mimics natural biological processes. This platform is promising for tuning surface properties or to introduce novel biofunctionalities via copolymerization with various functional units and/or combination with other materials. Important progress in this field in recent years is highlighted.

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Section: Biologically Related Applicationssupporting

confidence: 62%

Biomimetic Smart Interface Materials for Biological Applications

2011

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“…In a similar method, anti-human CD34 monoclonal antibodies adsorbed on a PIPAAm-grafted PP membrane enriched CD34-positive KG-1a cells to 85% from a 1 : 1 cell suspension of KG-1a cells and Jurkat cells. 110 These methods enable simple separation of cells; however, they use antibodies, which are sometimes expensive and unstable, leading to high costs and low reproducibility. Additionally, the separated cells contain antibodies on the cell surfaces, which leads to problems in research or in transplants to the human body.…”

Section: Cell-separating Materials Based On Thermoresponsive Polymersmentioning

confidence: 99%

Thermoresponsive-polymer-based materials for temperature-modulated bioanalysis and bioseparations

Nagase

Okano

2016

J. Mater. Chem. B

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“…Several proteins have been investigated for their interactions with PNIPAAm polymers including bovine serum albumin (BSA) [48,[59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74], human serum albumin (HSA) [75][76][77][78][79][80][81], fibrinogen (Fg) [82][83][84], lysozyme [85][86][87][88][89][90], immunoglobulin (IgG) [91][92][93], human gamma globulin (HGG) [94], myoglobin [95][96][97], assorted serum or plasma proteins [98,99], and recombinant proteins [60]. While these vary in structure, functionality, and sequence, a general finding is that they adsorb in greater quantities above the temperature at which the VPT occurs.…”

Section: Protein Interaction Dependence On Volume Phase Transition (Vpt)mentioning

confidence: 99%

Protein-surface interactions on stimuli-responsive polymeric biomaterials

2016

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Responsive surfaces: a review of the dependence of protein adsorption on the reversible volume phase transition in stimuli-responsive polymers. Specifically addressed are a widely studied subset: thermoresponsive polymers. Findings are also generalizable to other materials which undergo a similarly reversible volume phase transition. As of 2015, over 100,000 articles have been published on stimuli-responsive polymers and many more on protein-biomaterial interactions. Significantly, fewer than 100 of these have focused specifically on protein interactions with stimuli-responsive polymers. These report a clear trend of increased protein adsorption in the collapsed state compared to the swollen state. This control over protein interactions makes stimuli-responsive polymers highly useful in biomedical applications such as wound repair scaffolds, on-demand drug delivery, and antifouling surfaces. Outstanding questions are whether the protein adsorption is reversible with the volume phase transition and whether there is a time-dependence. A clear understanding of protein interactions with stimuli-responsive polymers will advance theoretical models, experimental results, and biomedical applications.

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Effective cell separation utilizing poly(N-isopropylacrylamide)-grafted polypropylene membrane containing adsorbed antibody

Cited by 10 publications

References 10 publications

Biomimetic Smart Interface Materials for Biological Applications

Biomimetic Smart Interface Materials for Biological Applications

Thermoresponsive-polymer-based materials for temperature-modulated bioanalysis and bioseparations

Protein-surface interactions on stimuli-responsive polymeric biomaterials

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