Identifying emotions has become essential for comprehending varied human behavior during our daily lives. The electroencephalogram (EEG) has been adopted for eliciting information in terms of waveform distribution over the scalp. The rationale behind this work is twofold. First, it aims to propose spectral, entropy and temporal biomarkers for emotion identification. Second, it aims to integrate the spectral, entropy and temporal biomarkers as a means of developing spectro-spatial ( S S ) , entropy-spatial ( E S ) and temporo-spatial ( T S ) emotional profiles over the brain regions. The EEGs of 40 healthy volunteer students from the University of Vienna were recorded while they viewed seven brief emotional video clips. Features using spectral analysis, entropy method and temporal feature were computed. Three stages of two-way analysis of variance (ANOVA) were undertaken so as to identify the emotional biomarkers and Pearson’s correlations were employed to determine the optimal explanatory profiles for emotional detection. The results evidence that the combination of applied spectral, entropy and temporal sets of features may provide and convey reliable biomarkers for identifying S S , E S and T S profiles relating to different emotional states over the brain areas. EEG biomarkers and profiles enable more comprehensive insights into various human behavior effects as an intervention on the brain.